Ark shell protein‐derived bioactive peptides promote osteoblastic differentiation through upregulation of the canonical Wnt/β‐catenin signaling in human bone marrow‐derived mesenchymal stem cells

Abstract

Bone is a dynamic organ that is tightly regulated by the process called bone remodeling, including bone formation by osteoblast and bone resorption by osteoclast (Ahn & Je, 2019). Osteoporosis is a common bone disease caused by imbalance between osteoblast activity and osteoclast activity, resulting in skeletal fractures and

LncRNA metastasis‐associated lung adenocarcinoma transcript‐1 promotes osteogenic differentiation of bone marrow stem cells and inhibits osteoclastic differentiation of Mø in osteoporosis via the miR‐124‐3p/IGF2BP1/Wnt/β‐catenin axis

“…IGF2BP1 is defined as a target of the Wnt/β-catenin pathway (Noubissi et al, 2018). Yunok Oh et al (2020) have elucidated that bioactive peptides derived from ark shell protein can facilitate osteoblastic differentiation by upregulating the Wnt/β-catenin signaling in human BMSCs. Our results showed that overexpression of miR-124-3p significantly reduced the protein levels of Wnt/β-catenin, while the effect could be reversed through upregulation of IGF2BP1.…”

“…It has been evidenced that the Wnt/β-catenin pathway promotes the osteogenic differentiation of BMSCs (Oh et al, 2020). Western blotting was performed to detect the activation of the Wnt/β-catenin pathway.…”

Anti‐inflammatory action of ark shell ( Scapharca subcrenata ) protein hydrolysate in LPS‐stimulated RAW264.7 murine macrophages

“…In the present study, enzymatic hydrolysis is selected to generate BAPs from ark shell using two proteolytic enzymes including Alcalase® and pepsin. These two enzymes are widely used for preparation of bioactive peptides or hydrolysates showing various biological activities such as anti-inflammatory, cytoprotective, and osteogenic effects (Da Silva, 2017;Gao et al, 2021;Meram & Wu, 2017;Oh et al, 2018Oh et al, , 2020Oh et al, , 2021. Then, we examined anti-inflammatory effect of ASAH and ASPH in LPS-stimulated RAW264.7 murine macrophages.…”

“…S. subscrenata, an ark shell, is a popular edible seafood in Korea, Japan, and China and inhabit in the coastal water which consists of muddy sediments (Nakamura, 2005). Recently, our group demonstrated that ark shell protein hydrolysates exhibited anti-adipogenic, antioxidant, osteogenic, and anti-osteoporotic effects (Hyung et al, 2017;Jin et al, 2018;Oh et al, 2019Oh et al, , 2020. However, anti-inflammatory properties of ark shell protein hydrolysates have not been investigated yet.…”

Isolation and identification of peptides from Pinctada martensii with osteogenic activity

“…The peptides are not active in the parent protein sequence, but are released following digestion or hydrolysis. Oh et al (2020) found that peptides derived from ark shell protein promoted osteoblast differentiation in hBMMSCs through upregulation of the canonical Wnt/b-catenin signalling and rescue ovariectomy-induced bone loss. Hyung et al (2018) examined the osteogenic effect of blue mussel (Mytilus edulis) protein hydrolysates (BMPH) on osteoblast differentiation by using mouse mesenchymal stem cells (MSCs).…”