Two novel antimicrobial defensins from rice identified by gene coexpression network analyses

Tantong, Supaluk; Pringsulaka, Onanong; Weerawanich, Kamonwan; Meeprasert, Arthitaya; Rungrotmongkol, Thanyada; Sarnthima, Rakrudee; Roytrakul, Sittiruk; Sirikantaramas, Supaart

doi:10.1016/j.peptides.2016.07.005

Cited by 35 publications

(39 citation statements)

References 63 publications

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“…Molecular protein-protein docking was performed using ZDOCK 3.0.2 (92) to predict the complex structure of OCT4 binding to WT CAV1 based on the parameters used in previous studies (55,93). From the 2000 docked structures, the WT CAV1:OCT4 complex with the highest ZDOCK score was retrieved and subsequently used to construct the phosphorylated Tyr-14 CAV1 (pTyr-14 CAV1) and Y14F CAV1 mutant in complex with OCT4.…”

Section: Methodsmentioning

confidence: 99%

Nitric oxide promotes cancer cell dedifferentiation by disrupting an Oct4:caveolin-1 complex: A new regulatory mechanism for cancer stem cell formation

Maiuthed

Bhummaphan

Luanpitpong

et al. 2018

Journal of Biological Chemistry

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Cancer stem cells (CSCs) are unique populations of cells that can self-renew and generate different cancer cell lineages. Although CSCs are believed to be a promising target for novel therapies, the specific mechanisms by which these putative therapeutics could intervene are less clear. Nitric oxide (NO) is a biological mediator frequently up-regulated in tumors and has been linked to cancer aggressiveness. Here, we search for targets of NO that could explain its activity. We find that it directly affects the stability and function of octamer-binding transcription factor 4 (Oct4), known to drive the stemness of lung cancer cells. We demonstrated that NO promotes the CSC-regulatory activity of Oct4 through a mechanism that involves complex formation between Oct4 and the scaffolding protein caveolin-1 (Cav-1). In the absence of NO, Oct4 forms a molecular complex with Cav-1, which promotes the ubiquitin-mediated proteasomal degradation of Oct4. NO promotes Akt-dependent phosphorylation of Cav-1 at tyrosine 14, disrupting the Cav-1:Oct4 complex. Site-directed mutagenesis and computational modeling studies revealed that the hydroxyl moiety at tyrosine 14 of Cav-1 is crucial for its interaction with Oct4. Both removal of the hydroxyl via mutation to phenylalanine and phosphorylation lead to an increase in binding free energy (Δ) between Oct4 and Cav-1, destabilizing the complex. Together, these results unveiled a novel mechanism of CSC regulation through NO-mediated stabilization of Oct4, a key stem cell transcription factor, and point to new opportunities to design CSC-related therapeutics.

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Section: Methodsmentioning

confidence: 99%

Nitric oxide promotes cancer cell dedifferentiation by disrupting an Oct4:caveolin-1 complex: A new regulatory mechanism for cancer stem cell formation

Maiuthed

Bhummaphan

Luanpitpong

et al. 2018

Journal of Biological Chemistry

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“…Tantong et al reported that OsDEF7, which is the OsAFP1 in our study, inhibited the growth of rice pathogenic bacteria, Xanthomonas oryzae and Erwinia carotovora. 11) Therefore, further analysis would be required to confirm the species-specific antifungal activities of OsAFP1 against bacteria.…”

Section: Resultsmentioning

confidence: 99%

“…11) These defensin proteins exhibited antifungal and antimicrobial activity against rice pathogens. According to The Rice Annotation Project Database, the OsAFP1 cloned in this study and OsDEF7 11) are on the same site of chromosome II and have the same amino acid sequence, suggesting that both genes are the same.…”

Section: Introductionmentioning

confidence: 82%

Partial peptides from rice defensin OsAFP1 exhibited antifungal activity against the rice blast pathogen <i>Pyricularia oryzae</i>

Sagehashi

Takaku

Yatou

2017

Journal of Pesticide Science

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Rice blast caused by Pyricularia oryzae is one of the most devastating diseases worldwide. This study aimed to investigate the antifungal activity of rice defensin OsAFP1 and its partial peptides against P. oryzae. The partial peptides near the N-and C-terminal regions of OsAFP1 exhibited approximately the same antifungal activity as the entire protein against P. oryzae. These partial peptides have the potential to be used as fungicides.

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“…AMPs are known to play important roles in constitutive or induced resistance to various pathogens, by degrading fungal cell walls, inducing membrane channel and pore formation, inhibiting DNA synthesis and cell cycle, and damaging cellular ribosomes [3−7]. Plant AMPs have been shown to enhance tolerance to many fungal diseases in several species, including pepper [8], rice [7,9,10], potato [11], tobacco [3], and creeping bentgrass and citrus [12,13]. In addition, a defensin from chickpea, which is a type of AMP, confers tolerance against water de cit stress in Arabidopsis thaliana [14].…”

Section: Introductionmentioning

confidence: 99%

“…atroseptica, and weaken the activity of the phytopathogenic fungi Helminthosporium oryzae and F. oxysporum f. sp. cubense [7,9]. Protein extracts with MSI-99, an AMP expressed in chloroplasts of tobacco, could signi cantly suppress two rice blast isolates, both in vitro and in vivo [10].…”

Section: Introductionmentioning

confidence: 99%

Enhanced tolerance to Phytophthora root and stem rot by over-expression of the plant antimicrobial peptide CaAMP1 gene in soybean

Lü

Zhong

Zhang

et al. 2020

Preprint

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Background: Antimicrobial peptides play important roles in both plant and animal defense systems. Moreover, over-expression of CaAMP1 (Capsicum annuum antimicrobial protein 1), an antimicrobial protein gene isolated from C. annuum leaves infected with Xanthomonas campestris pv. vesicatoria, confers broad-spectrum resistance to hemibiotrophic bacterial and necrotrophic fungal pathogens in Arabidopsis. Phytophthora root and stem rot (PRR), caused by the fungus Phytophthora sojae, is one of the most devastating diseases affecting soybean (Glycine max) production worldwide.Results: In this study, CaAMP1 was transformed into soybean by Agrobacterium-mediated genetic transformation. Integration of the foreign gene in the genome of transgenic soybean plants and its expression at the translation level were verified by Southern and western blot analyses, respectively. CaAMP1 over-expression (CaAMP1-OX) lines inoculated with P. sojae race 1 exhibited enhanced and stable PRR tolerance through T2–T4 generations compared with the wild-type Williams 82 plants. Gene expression analyses in the transgenic plants revealed that the expression of salicylic acid-dependent, jasmonic acid-dependent, and plant disease resistance (R-genes) genes were significantly up-regulated after P. sojae inoculation.Conclusions: These results indicate that CaAMP1 over-expression can significantly enhance PRR tolerance in soybean by eliciting resistance responses mediated by multiple defense signaling pathways. This provides an alternative approach for developing soybean varieties with improved tolerance against soil-borne pathogenic PRR.

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Two novel antimicrobial defensins from rice identified by gene coexpression network analyses

Cited by 35 publications

References 63 publications

Nitric oxide promotes cancer cell dedifferentiation by disrupting an Oct4:caveolin-1 complex: A new regulatory mechanism for cancer stem cell formation

Nitric oxide promotes cancer cell dedifferentiation by disrupting an Oct4:caveolin-1 complex: A new regulatory mechanism for cancer stem cell formation

Partial peptides from rice defensin OsAFP1 exhibited antifungal activity against the rice blast pathogen <i>Pyricularia oryzae</i>

Enhanced tolerance to Phytophthora root and stem rot by over-expression of the plant antimicrobial peptide CaAMP1 gene in soybean

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