Partial peptides from rice defensin OsAFP1 exhibited antifungal activity against the rice blast pathogen &lt;i&gt;Pyricularia oryzae&lt;/i&gt;

Sagehashi, Yoshiyuki; Takaku, Hiroaki; Yatou, Osamu

doi:10.1584/jpestics.d17-046

Cited by 10 publications

(8 citation statements)

References 19 publications

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“…The following defensins of rice Oryza sativa L. were studied in detail: OsDEF7 and OsDEF8 by Tantong et al [ 67 ] and Weerawanich et al [ 93 ]; OsDEF7, named OsAFP1, was further studied by Sagehashi et al [ 94 ] and Ochiai et al [ 95 ], while Cal1, identical to OsDEF7, was analyzed by Luo et al [ 96 ].…”

Section: Defensins Of Grassesmentioning

confidence: 99%

“…The inhibitory activity of OsDEF7 named OsAFP1 against the rice blast pathogen Pyricularia oryzae was studied by Sagehashi et al [ 94 ]. The recombinant peptide was expressed in E. coli as a fusion protein with GST.…”

Section: Defensins Of Grassesmentioning

confidence: 99%

“…Structure–function relationships in rice defensins were studied using OsDEF7 (OsAFP1) as a model [ 94 , 95 ]. Eight short peptides covering the entire sequence of OsDEF7 were synthesized.…”

Section: Defensins Of Grassesmentioning

confidence: 99%

“…Eight short peptides covering the entire sequence of OsDEF7 were synthesized. The inhibitory activity of the short peptides was assayed against the plant ( P. oryzae ) and human ( C. albicans ) pathogens [ 94 , 95 ]. Peptides 1 (residues 1‒10), 2 (residues 5‒15), 7 (residues 35‒44), and 8 (residues 40‒49) derived from the N-and C-terminal regions of OsDEF7 displayed antimicrobial activity against P. oryzae (IC 50 = 0.41‒1.42 µg/mL).…”

Section: Defensins Of Grassesmentioning

confidence: 99%

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Defensins of Grasses: A Systematic Review

Одинцова

Slezina

2020

Biomolecules

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The grass family (Poaceae) is one of the largest families of flowering plants, growing in all climatic zones of all continents, which includes species of exceptional economic importance. The high adaptability of grasses to adverse environmental factors implies the existence of efficient resistance mechanisms that involve the production of antimicrobial peptides (AMPs). Of plant AMPs, defensins represent one of the largest and best-studied families. Although wheat and barley seed γ-thionins were the first defensins isolated from plants, the functional characterization of grass defensins is still in its infancy. In this review, we summarize the current knowledge of the characterized defensins from cultivated and selected wild-growing grasses. For each species, isolation of defensins or production by heterologous expression, peptide structure, biological activity, and structure–function relationship are described, along with the gene expression data. We also provide our results on in silico mining of defensin-like sequences in the genomes of all described grass species and discuss their potential functions. The data presented will form the basis for elucidation of the mode of action of grass defensins and high adaptability of grasses to environmental stress and will provide novel potent molecules for practical use in medicine and agriculture.

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Section: Defensins Of Grassesmentioning

confidence: 99%

Section: Defensins Of Grassesmentioning

confidence: 99%

Section: Defensins Of Grassesmentioning

confidence: 99%

Section: Defensins Of Grassesmentioning

confidence: 99%

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Defensins of Grasses: A Systematic Review

Одинцова

Slezina

2020

Biomolecules

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“…In this study, antifungal activity assays against Candida albicans , the most common pathogenic fungal species that causes candidiasis in humans 20 , revealed that 4 μM DEF7 completely inhibited its proliferation. In a previous study, DEF7 (designated OsAFP1 in subsequent papers) was reported to exhibit antimicrobial activity against rice pathogenic microbes, such as Pyricularia oryzae 21 and Helminthosporium oryzae 22 . Based on this latest knowledge, basic functional analyses of O. sativa OsAFP1 were conducted to assess its potency as a novel antifungal drug.…”

Section: Introductionmentioning

confidence: 99%

Rice Defensin OsAFP1 is a New Drug Candidate against Human Pathogenic Fungi

Ochiai

Ogawa

Fukuda

et al. 2018

Sci Rep

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Fungal infections, such as candidiasis and aspergillosis, are some of the most frequent infections in humans. Although antifungal drugs are available for the treatment of these infections, antifungal agents with new mechanisms of action should be developed because of the increasing incidence of drug-resistant pathogens in recent years. In this study, a basic functional analysis of rice defensin OsAFP1, a novel antifungal drug candidate, was conducted. OsAFP1 exerted fungicidal activity against Candida albicans, the most common pathogenic fungus in humans, at 4 μM concentration, but it did not inhibit the growth of human pathogenic bacteria. In addition, OsAFP1 retained structural stability after heat treatment at 100 °C for 10 min and after serum treatment at 37 °C for 24 h. A propidium iodide (PI) uptake assay and mutational analysis revealed that amino acid residues within the C-terminal γ-core motif of OsAFP1, particularly Leu-39 and Lys-41, play an important role in its antifungal activity. Further, PI uptake and apoptosis assays suggested that OsAFP1 exerts its antifungal activity by inducing apoptosis of target cells. Immunohistochemistry showed that the OsAFP1 target molecule was located in the cell wall. These findings indicate that OsAFP1 may be developed into a potent antifungal drug.

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