Two new species of Tipula (Vestiplex) from Southern China based on morphological and molecular data, with redescription of Tipula (Vestiplex) bicalcarata (Diptera, Tipulidae, Tipulinae)

Abstract

Two new species of subgenus Tipula (Vestiplex) Bezzi, 1924, Tipula (Vestiplex) leigongshanensis Men & Young, sp. n. and Tipula (Vestiplex) maoershanensis Men & Young, sp. n. are described and illustrated. Tipula (Vestiplex) bicalcarata Savchenko, 1965 is redescribed and illustrated based on additional morphological characters. Partial mitochondrial cytochrome oxidase subunit I (COI) sequences of these three species are provided. Pairwise genetic distances among two new species and related species, Tipula (Vestiplex) bicalcarata, Tipula (Vestiplex) coxitalis Alexander, 1935, and Tipula (Vestiplex) sternotuberculata Alexander, 1935 range from 0.028 to 0.091 using Kimura-2-parameter model. Diagnostic features of the sperm pump for taxonomic use are discussed.

Six new species of Tipula (Vestiplex) Bezzi (Diptera, Tipulidae) from Tibet

“…The new species is similar to T. (V.) coxitalis Alexander, 1935 from Anhui and Taiwan, China, but the latter differs in the following characters: gonocoxite ends with a slightly twisted spine; outer gonostylus narrow, filiform; inner gonostylus without slanted bar-like raise; sternite 9 near caudal end on either side of midline with a conspicuous hair pencil of reddish bristles, these pencils not arising from basal tubercles (Men 2015). This species is similar to T. (V.) bicalcarata Savchenko, 1965 and T. (V.) maoershanensis Men & Young, 2017, but the outer gonostylus of the latter is not willow leaf shaped. The posterior margin of inner gonostylus with a finger-like protrusion (Men et al 2017).…”

“…This species is similar to T. (V.) bicalcarata Savchenko, 1965 and T. (V.) maoershanensis Men & Young, 2017, but the outer gonostylus of the latter is not willow leaf shaped. The posterior margin of inner gonostylus with a finger-like protrusion (Men et al 2017). Brunetti, 1911 Figs 51-57, 86 Tipula himalayensis Brunetti, 1911: 252.…”

“…The male tergite 9 commonly forms a shallowly concave sclerotized saucer or can be longitudinally divided into two parts, some species have two pubescent finger-like arms. The female ovipositor is commonly with saerrated outer margin (Alexander 1935(Alexander , 1965Savchenko 1964;Alexander & Byers 1981;Starkevich & Podenas 2011;Starkevich 2012;Men et al 2017).…”

Four new crane fly species of the subgenus Vestiplex (Diptera: Tipulidae: Tipula) from Tibet and Yunnan, China

“…A total of 76 species and one subspecies of Vestiplex are documented in China, which are mainly associated with the Tibetan Plateu (Tibet, Sichuan, Yunnan, Qinghai and other provinces) (Oosterbroek 2021). Among them, 20 species were described in the last six years (Men et al 2017(Men et al , 2021a(Men et al , 2021b(Men et al , 2021cStarkevich et al 2019Starkevich et al a, 2019bRen et al 2021 andYang et al 2021).…”

Two new crane fly species of the subgenus Vestiplex Bezzi, 1924 (Diptera, Tipulidae, Tipula) from Yunnan and Sichuan, China, with a key to species in the immota species group

“…The hypogynial valve is short to rudimentary, in the shape of blades or plates, or filamentous (Starkevich et al 2019a(Starkevich et al , 2020. Some species of the subgenus Vestiplex have tergite 9 of the male hypopygium forming a shallowly concave and sclerotized saucer (Alexander 1935;Alexander and Byers 1981;Starkevich et al 2020), while other species have tergite 9 divided by a pale membrane at the midline, with the posterior margin of the dorsal portion simple or bearing additional lobes, ventrally membranous or terminating in a pair of plates, sclerotized processes, armatures or flattened lobes (Alexander 1935;Alexander and Byers 1981;Men et al 2017;Pilipenko et al 2019;Starkevich et al 2019a, b).…”