Two New Colorimetric Methods for Early Detection of Vancomycin and Oxacillin Resistance in
            <i>Staphylococcus aureus</i>

Çoban, Ahmet Yılmaz; Bozdoğan, Bülent; Cihan, Cigdem Cekic; E, Cetinkaya; Bılgın, Kemal; Darka, Özge; Akgunes, Alper; Durupinar, Belma; Appelbaum, Peter C.

doi:10.1128/jcm.44.2.580-582.2006

Cited by 19 publications

(21 citation statements)

References 8 publications

Supporting

Mentioning

Contrasting

Unclassified

Order By: Relevance

“…Plates were incubated at 35°C after inoculation. Fifty microliters of freshly prepared reagent [2 units of 0.1% N-(1-naphthyl)ethylenediamine, 2 units of 0.2% sulfanilamide, and 1 unit of concentrated hydrochloric acid] was added into control wells at the fifth hour of incubation (6). It was considered that there was sufficient bacterial growth if a violetpurple color change was seen, and then the same amount of reagent was added to antibiotic-containing wells.…”

Section: Bacterial Isolatesmentioning

confidence: 99%

Rapid Determination of Methicillin Resistance among Staphylococcus aureus Clinical Isolates by Colorimetric Methods

Çoban

2012

J Clin Microbiol

View full text Add to dashboard Cite

In the present study, the effectiveness of a rapid and colorimetric nitrate reductase analysis (NRA) method and resazurin microplate assay (REMA) for rapid determination of methicillin resistance among Staphylococcus aureus was investigated. A total of 275 clinical isolates of S. aureus were included in the present study. Among these isolates, 151 had the mecA gene and were resistant to methicillin. The remaining 124 isolates were methicillin susceptible and did not have the mecA gene. Cefoxitin MICs of all isolates were detected by NRA, REMA, and reference broth microdilution methods. Category and essential agreement were determined as 100% and 99.6%, respectively, comparing both NRA and REMA with the reference method. No minor, major, or very major discrepancy was observed for either of the methods. The time necessary to have the MIC results was 5 h for NRA, whereas it was 6 h for REMA. Early detection of MRSA is an important public health concern, and the results of this study showed that both of the colorimetric methods are easy to perform and save time in the determination of MRSA. These methods have a potential use for early detection of MRSA for laboratories unable to use molecular techniques.

show abstract

Section: Bacterial Isolatesmentioning

confidence: 99%

Rapid Determination of Methicillin Resistance among Staphylococcus aureus Clinical Isolates by Colorimetric Methods

Çoban

2012

J Clin Microbiol

View full text Add to dashboard Cite

show abstract

“…The application of this method in clinical microbiology laboratories is very practical and easy, and allows the evaluation of methicillin resistance only 6 hours after the identification of S. aureus (after Gram staining and coagulase testing). Several studies compared methods that rely on liquid microdilution and colorimetric to determine oxacillin and vancomycin resistance in S. aureus isolates, and colorimetric methods are also used to detect antibiotic resistance in Mycobacterium tuberculosis (22)(23)(24)(25).…”

Section: Discussionmentioning

confidence: 99%

Same-day Detection of Methicillin Resistance in Staphylococcus aureus Isolates by StaResMet® Kit

Sezgin

Vural

Kiraz

et al. 2017

Jundishapur J Microbiol

View full text Add to dashboard Cite

Background: Staphylococcus aureus is an important cause of hospital-acquired infections. The most important issue with S. aureus is that the isolates are getting increasingly methicillin-resistant. Rapid differentiation between methicillin-resistant and methicillinsusceptible S. aureus species is necessary to optimize treatment and minimize costs. Objectives: The current study aimed at evaluating the StaResMet® kit for rapid detection of methicillin resistance in S. aureus isolates. Methods: A total of 217 methicillin-resistant S. aureus (MRSA) and 252 methicillin-susceptible S. aureus (MSSA) isolates were tested using the StaResMet® kit. The test was performed according to the manufacturer's instructions. Results: The kit identified the MRSA isolates with 100% accuracy, and found that the minimum inhibitory concentrations (MICs)

show abstract

“…Unfortunately, susceptibility testing by these methods requires 24 h (CLSI 2008). Recently, colorimetric media which yield susceptibility tasting in approximately 5-10 h were developed and started to use (Coban et al 2006;Ercis et al 2007). Quicolor agar (QC agar; Salubris Inc., Massachusetts; www.salubris.com), is one of these media which changes its color within 4-6 h due to metabolic activities of proliferating bacteria.…”

Section: Introductionmentioning

confidence: 99%

Rapid detection of methicillin resistance in Staphylococcus aureus isolates; evaluation of colorimetric Quicolor ES agar and determination of breakpoint inhibition zone diameters of cefoxitin

Çoban

Demirpek

Yıldırım

et al. 2011

World J Microbiol Biotechnol

View full text Add to dashboard Cite

In this study, it was aimed to evaluate colorimetric Quicolor ES agar for the rapid detection of methicillin resistance and to determine susceptibility and resistance breakpoint zone diameters for cefoxitin by using 51 methicillin susceptible Staphylococcus aureus (MSSA) and 63 methicillin resistant S. aureus (MRSA) isolates. In the study, while oxacillin and cefoxitin results were obtained within 4-7 h (5.5 h in average) for MSSA isolates, the results of MRSA isolates were obtained within 5.5-9 h (6.6 h in average) for both antibiotics on QC ES agar. QC ES agar is an inexpensive medium for rapid detection (4-9 h) of methicillin resistance by disc diffusion method using oxacillin or cefoxitin. Additional studies for further evaluation of the efficiency of QC-ES agar in rapid determination of methicillin resistance in S. aureus may be beneficial.

show abstract

Two New Colorimetric Methods for Early Detection of Vancomycin and Oxacillin Resistance in Staphylococcus aureus

Cited by 19 publications

References 8 publications

Rapid Determination of Methicillin Resistance among Staphylococcus aureus Clinical Isolates by Colorimetric Methods

Rapid Determination of Methicillin Resistance among Staphylococcus aureus Clinical Isolates by Colorimetric Methods

Same-day Detection of Methicillin Resistance in Staphylococcus aureus Isolates by StaResMet® Kit

Rapid detection of methicillin resistance in Staphylococcus aureus isolates; evaluation of colorimetric Quicolor ES agar and determination of breakpoint inhibition zone diameters of cefoxitin

Contact Info

Product

Resources

About