Same-day Detection of Methicillin Resistance in Staphylococcus aureus Isolates by StaResMet® Kit

Sezgin, Fikriye Milletli; Vural, Ahmet; Kiraz, Aslı Öztürk; Çaycı, Yeliz Tanrıverdi; Coban, Cagri; Bozdoğan, Bülent; Çoban, Ahmet Yılmaz

doi:10.5812/jjm.14937

Cited by 3 publications

(1 citation statement)

References 26 publications

(26 reference statements)

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“…Methicillin-resistant S. aureus (MRSA) and methicillin-sensitive S. aureus (MSSA) isolates were tested, and the MRSA and MSSA isolates were identified with 100% accuracy. The StaResMet ® kit detects MRSA isolates quickly and reliably and could be a valuable option for microbiology laboratories with limited resources (23).…”

Section: Discussionmentioning

confidence: 99%

A New Colorimetric Method for Rapid Detection of Antibiotic Resistance in Escherichia coli Isolates

Gun¹,

Çaycı²,

Durupinar³

et al. 2022

Jundishapur J Microbiol

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Background: The quick diagnosis and early initiation of antibiotic therapy in bacteria-induced infections is of paramount importance. Accordingly, the rapid identification of the causative agent, the short-term results of antibiotic sensitivity, the selection and use of right antibiotics for treatment further highlights the significance of this issue. Objectives: This study aimed to develop a new susceptibility testing method to provide rapid results in Escherichia coli clinical isolates and report the antibiotic susceptibility test results to clinicians in a short period. Methods: In the study, one hundred and ten E. coli clinical isolates were tested. In this regard, antibiotics recommended by the "Clinical and Laboratory Standards Institute (CLSI)" for testing the sensitivity of E. coli isolates, including amoxicillin-clavulanate, cefixime, ceftriaxone, ertapenem, ciprofloxacin, gentamicin, trimethoprim-sulfamethoxazole, and nitrofurantoin were tested. For quality control, E. coli ATCC25922, E. coli ATCC35218, Staphylococcus aureus ATCC29213, and E. coli 13846NTCC strains were used. The broth microdilution method recommended by CLSI was used as the reference method. Minimum inhibitory concentration values were determined, and antimicrobial susceptibilities were then determined according to the “European Committee on Antimicrobial Susceptibility Testing (EUCAST)” criteria. In the next phase, the results of the resazurin microplate method (RMM) were compared. Results: The comparison of the RMM developed in the present study with the reference method revealed that the calculated essential agreement ratios for eight antibiotics varied from 82.72 to 100%, and the categorical agreement values ranged from 95.45 to100%. Conclusions: According to the findings, the RMM results were highly in agreement with the results of the reference method. RMM allows the detection of antibiotic susceptibility quickly (e.g., within 5 hours) as such it is preferred, especially for laboratories with limited facilities. However, further multi-center studies are recommended to use this method in routine laboratories.

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Section: Discussionmentioning

confidence: 99%

A New Colorimetric Method for Rapid Detection of Antibiotic Resistance in Escherichia coli Isolates

Gun¹,

Çaycı²,

Durupinar³

et al. 2022

Jundishapur J Microbiol

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Nitrate Reductase Assay for Rapid Determination of Methicillin-Resistant Staphylococcus aureus Clinical Isolates

Yildirim,

Atas,

Tanyel Akcit

et al. 2023

Laboratory Medicine

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Objective To evaluate the performance of nitrate reductase assay (NRA), a rapid, colorimetric method for the determination of methicillin resistance in Staphylococcus aureus isolates obtained from the culture collection of the Akdeniz University Hospital Central Laboratory, Antalya, Türkiye. Materials and Methods Identification for all 290 S aureus isolates at the species level was performed via matrix-assisted laser desorption/ionization-time of flight. Isolates were tested with NRA for methicillin resistance. The cefoxitin broth microdilution (BMD) method recommended by the Clinical and Laboratory Standards Institute was used as the reference method in the study. S aureus ATCC 29213 and S aureus ATCC 43300 strains were used for quality control. Results According to Food and Drug Administration criteria, the category agreement between NRA and BMD was found to be 100%. The essential agreement between both methods was determined to be 96.20%. There is no minor, major, or extremely major discrepancy between both methods. Conclusion The results show that NRA is a rapid, practical, and reliable colorimetric method for detecting MRSA.

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METİSİLİNE DİRENÇLİ STAPHYLOCOCCUS AUREUS KLİNİK İZOLATLARININ HIZLI BELİRLENMESİ İÇİN StaResMet®’İN PERFORMANSININ DEĞERLENDİRİLMESİ

YILDIRIM

2023

ANKEM Dergisi

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Metisilin direncinin hızlı ve doğru tespiti, Staphylococcus aureus ile gelişen enfeksiyonların tedavisinde önemli bir aşamadır. Bu çalışmada, Akdeniz Üniversitesi Hastanesi Merkez Laboratuvarı kültür koleksiyonundan temin edilen S. aureus izolatlarında metisilin direncinin hızlı tespiti için StaResMet® kiti (AYCMED Medikal ve Tıbbi Cihazlar San. ve Tic. A.Ş., Samsun, Türkiye) değerlendirilmiştir. Tür düzeyinde tanımlamaları MALDİ-TOF (Becton Dickinson, BD) ile yapılarak seçilen 290 adet S. aureus izolatı, StaResMet® kiti ile metisilin direnci açısından test edilmiştir. Çalışmada referans yöntem olarak CLSI tarafından önerilen sıvı mikrodilüsyon (SMD) yöntemi kullanılarak sefoksitin minimum inhibitör konsantrasyonu (MİK) belirlenmiştir. Test yöntemleri eş zamanlı olarak, aynı bakteriyel inokulumlar kullanılarak gerçekleştirilmiştir. Kalite kontrol için S. aureus ATCC 29213 (metisiline duyarlı) ve S. aureus ATCC 43300 (metisiline dirençli) suşları kullanılmıştır. CLSI kriterlerine göre MİK ≤4 μg/ml olan suşlar, metisiline duyarlı; MİK ≥8 μg/ml olan suşlar ise metisiline dirençli kabul edilmiştir. StaResMet® ile tüm izolatların inkübasyonun altıncı saatinde duyarlılık sonuçları belirlenmiş ve SMD ile 20 saatin sonunda elde edilen duyarlılık sonuçları ile karşılaştırılmıştır. StaResMet® kiti ile alınan duyarlılık sonuçları, SMD ile kategorik olarak 100 % uyumlu bulunmuştur. Referans yöntem ile StaResMet® kit arasında temel uyum oranı 98.27 % olarak saptanmıştır. Her iki yöntem arasında da küçük, büyük veya çok büyük uyumsuzluk bulunmamıştır. StaResMet®, metisiline dirençli Staphylococcus aureus’un saptanmasında hızlı, kullanımı kolay, güvenilir bir kolorimetrik ilaç duyarlılık kiti olup, klinik uygulamalarda kullanılma potansiyelinin yüksek olduğu düşünülmüştür.

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Same-day Detection of Methicillin Resistance in Staphylococcus aureus Isolates by StaResMet® Kit

Cited by 3 publications

References 26 publications

A New Colorimetric Method for Rapid Detection of Antibiotic Resistance in Escherichia coli Isolates

A New Colorimetric Method for Rapid Detection of Antibiotic Resistance in Escherichia coli Isolates

Nitrate Reductase Assay for Rapid Determination of Methicillin-Resistant Staphylococcus aureus Clinical Isolates

METİSİLİNE DİRENÇLİ STAPHYLOCOCCUS AUREUS KLİNİK İZOLATLARININ HIZLI BELİRLENMESİ İÇİN StaResMet®’İN PERFORMANSININ DEĞERLENDİRİLMESİ

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