Two monoclonal antibodies specific for the T cell receptor V_α8

Abstract

Two monoclonal antibodies, KT50 and KT65, specific for V alpha 8 have been established. This was determined as follows: (a) 4 T cell clones, C6, R1, G22 and I9, out of 43 T cell clones with various antigen specificities, major histocompatibility complex restrictions and V beta usages not only bound KT50 and KT65 but also expressed V alpha 8 mRNA, (b) KT50 and KT65 precipitated molecules from the clone C6 similar to the T cell receptor molecules precipitated in C6 cells by KT11 (anti-V beta 11) or KTL2 (anti-Ti) and (c) KT50 and KT65 were mitogenic and induced cytotoxicity. All strains of mice so far examined have populations of KT50+ and KT65+ T cells of 1.4%-3.6% and 0.9%-2.6%, respectively. Different H-2 haplotypes were not observed to affect the number of cells expressing KT50 or KT65. In addition KT15 (anti-CD8), without cross-linking to KT50 or KT65, augmented proliferation triggered by KT50 or KT65.

Monoclonal antibodies raised against engineered soluble mouse T cell receptors and specific for Vα8‐, Vβ2‐ or Vβ10‐bearing T cells

“…Moreover, analysis of the pattern of staining of the 1BR124, 3Q21 or 1Q74 hybridomas indicates that the determinants recognized by B21.13 and B21.14 are distinct and may be expressed independently of each other (Table 1). Therefore, as previously observed for mAb with specificity for V,3.2 [29],V,8 [30] and V,11 [31] products, B21.13 and B21.14 each react with only some members of the Va8 subfamily. Such a restricted pattern of reactivity may account for the fact that B21.14 only stains from 2-4% of the LN cells found in the mouse strains shown in Table 2.…”

“…Such a restricted pattern of reactivity may account for the fact that B21.14 only stains from 2-4% of the LN cells found in the mouse strains shown in Table 2. Due to the presence of five functional members in the Va8 subfamily [32] and to the existence of a high degree of interstrain V, polymorphism [5, 331, analysis of transfectants which express each of the functional members of the V,8 subfamily will be necessary to determine the structural relationship among the epitopes recognized by B21.13, B21.14 and the two anti-Va8 mAb previously identified by Tomonari et al [30].…”

Vβ gene repertoires in T cells expanded in local self‐healing and lethal systemic murine cutaneous leishmaniasis

“…Three-color staining of lymph node cells (LNC) was performed using anti-Va8 mAb (SN of hybridoma KT.50, [13], a kind gift of Dr. Tomonari, Harrow, GB) detected with biotinylated donkey anti-rat IgG (Dianova, Hamburg, FRG) and streptavidin-conjugated TRI-COLOR (Medac, Hamburg, FRG). After adsorption with rat IgG, the cells were stained with anti-CD4-PE (Becton-Dickinson) and FITC-conjugated anti-Vp4 mAb ( [14], a gift of Dr. Tomonari).…”

Direct binding of secreted T-cell receptor beta chain to superantigen associated with class II major histocompatibility complex protein.

“…No superantigen-like effect has been noted for TCR Vas. These do not seem to be subject to the massive deletions common to many Vps (10)(11)(12). All of the superantigens require MHC class II expression to be recognized by T cells (2-4, 13, 14).…”