Monoclonal antibodies raised against engineered soluble mouse T cell receptors and specific for V_α8‐, V_β2‐ or V_β10‐bearing T cells

Abstract

We have characterized a panel of monoclonal antibodies (mAb) produced by immunizing rats with two distinct soluble mouse alpha/beta T cell receptor (TcR). Fifty mAb were found to react with the corresponding surface-bound TcR. Such observations suggest that the soluble TcR molecules used as immunogen are folded in a conformation similar to the native structure. Furthermore, the binding to T cells of four antibodies was found to correlate with the expression of the V alpha 8, V beta 2 or V beta 10 gene segments. Finally, staining of T lymphocytes from various mouse strains suggests that (a) the two anti-V alpha 8 antibodies recognize different epitopes, and each on only a fraction of V alpha 8+ cells; (b) the anti-V beta 10 mAb identifies a V beta 10 polymorphism among mouse strains, and (c) T cells expressing the V beta 2 or V beta 10 gene segments are not subject to major clonal deletion events induced by the major histocompatibility complex class II and Mls products which were tested.

CD4+ beta islet cell-reactive T cell clones that suppress autoimmune diabetes in nonobese diabetic mice.

“…NOD/Lt mice were bred at the animal facility at Dana-Farber Cancer Institute (Boston, MA). The following mAbs were used: anti-I-A k' r. s. ~ g7 (10-2.16, [21]); anti-I-A a (MK-D6, [22]), anti-Ib4 (11b11, [23]); anti-IL-2 receptor (PC 61 5.3; TIB-222; American Type Culture CoLlection, RockviLle, MD); anti-V~2 (B20.6, [24]); anti-V/~8 (F23.1, [25]); and anti-VB14 (14-2, [26]). The peptides used, Pl (yTYTVHAAHAYTYt; small letters denote D amino acids) and P2 (KMKMVH-AAHAKMKM) (27), were synthesized at Dana-Farber Cancer Institute core facility.…”

Characterization of T cell receptor single‐chain Fv fragments secreted by myeloma cells

“…B20.6 is a rat mAb (IgG2a) specific for the product of the mouse Vp2 gene segment [6]. B21.14 is a rat mAb (IgG1) specific for the product of some of the mouse Va8 gene segments [22]. B21.5 (rat IgG) and B21-7D4.…”

“…B21.5 (rat IgG) and B21-7D4. (rat IgG2a) are two mAb specific for the product of the mouse VplO gene segment [22].…”

T cell receptor α‐chain tail is required for protein kinase C‐mediated down‐regulation, but not for signaling

“…The anti-Va2.1 specific mAb B20.1 [28], the anti-VP8.l mAb F23.1 [29] and the anti-CD3e mAb 145-2~11 [30] were purified from culture supernatants using protein G (Pharmacia, Uppsala, Sweden). The anti-t chain mAb G3 [31] was a generous gift from N. van Oers (University of California, San Francisco, CA).…”