Direct binding of secreted T-cell receptor beta chain to superantigen associated with class II major histocompatibility complex protein.

Gascoigne, Nicholas R. J.; Ames, Kristina

doi:10.1073/pnas.88.2.613

Cited by 77 publications

(36 citation statements)

References 36 publications

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“…In contrast, mature DCs override the need for nef seen with the immature DCs and signal sufficiently to promote ⌬nef replication. Mature DCs express higher levels of costimulatory and adhesion molecules (17,20,42), which could facilitate binding between the DCs and T cells without the need for nef or SEB in this setting. nef has been shown to have superantigenlike qualities (57,58), and it could be acting in a similar way to SEB in our cultures to drive virus replication.…”

Section: Discussionmentioning

confidence: 99%

The Decreased Replicative Capacity of Simian Immunodeficiency Virus SIVmac239ΔnefIs Manifest in Cultures of Immature Dendritic Cells and T Cells

Messmer

Ignatius

Santisteban

et al. 2000

J Virol

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Transmission of simian immunodeficiency virus SIVmac239⌬nef (⌬nef) to macaques results in attenuated replication of the virus in most animals and ultimately induces protection against challenge with some pathogenic, wild-type SIV strains. It has been difficult, however, to identify a culture system in which the replication of ⌬nef is severely reduced relative to that of the wild type. We have utilized a primary culture system consisting of blood-derived dendritic cells (DCs) and autologous T cells. When the DCs were fully differentiated or mature, the DC-CD4 ؉ T-cell mixtures supported replication of both the parental SIV strain, 239 (the wild type), and its mutant with nef deleted (⌬nef), irrespective of virus dose and the cell type introducing the virus to the coculture. In contrast, when immature DCs were exposed to ⌬nef and cocultured with T cells, virus replication was significantly lower than that of the wild type. Activation of the cultures with a superantigen allowed both ⌬nef and the wild type to replicate comparably in immature DC-T-cell cultures. Immature DCs, which, it has been hypothesized, capture and transmit SIV in vivo, are deficient in supporting replication of ⌬nef in vitro and may contribute to the reduced pathogenicity of ⌬nef in vivo.

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Section: Discussionmentioning

confidence: 99%

The Decreased Replicative Capacity of Simian Immunodeficiency Virus SIVmac239ΔnefIs Manifest in Cultures of Immature Dendritic Cells and T Cells

Messmer

Ignatius

Santisteban

et al. 2000

J Virol

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“…Since it is known that bacterial superantigens bind HLA-DR antigens of APC in an MHC-unrestricted manner and stimulate a large population of certain Vb T cells [31], purified HLA-DR -…”

Section: Il-12 Is Required For Ifn-g Production From Cd4 þ T Cells Stmentioning

confidence: 99%

Inhibition of IL-12 synthesis of peripheral blood mononuclear cells (PBMC) stimulated with a bacterial superantigen by pooled human immunoglobulin: implications for its effect on Kawasaki disease (KD)

Takata

Seki²,

Dobashi

et al. 1998

Clinical and Experimental Immunology

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SUMMARYThe aim of this study was to further assess the role of pooled human immunoglobulin (PHIG) on cytokine production from PBMC stimulated with a bacterial superantigen. Human PBMC were cultured with Streptococcus pyrogenic exotoxin A (SPE-A) with or without PHIG and several proinflammatory cytokine levels of culture supernatants were measured. Serum cytokine levels of KD patients before and after PHIG therapy were also examined. PHIG greatly reduced the production of IL-12, interferongamma (IFN-g) and other cytokines from SPE-A-stimulated PBMC, while exogenous IL-12, but neither IL-1 nor tumour necrosis factor-alpha (TNF-a), restored IFN-g production inhibited by PHIG. Although PHIG partially adsorbed SPE-A, its inhibitory effect on cytokine production was not played by anti-SPE-A antibody. Although purified CD4þ T cells cultured with human HLA-DR-transfected mouse L cells and SPE-A could not effectively produce IFN-g, they produced large amounts of IFN-g if exogenous IL-12 was introduced. KD patients in the acute phase had higher levels of serum IFN-g than did controls and patients with bacterial infection. Although IL-12 levels of children with or without KD were not significantly different, IL-12 levels of children were much higher than those of adults. However, serum levels of IL-12 of KD patients were transiently but significantly decreased by PHIG therapy and IFN-g amounts subsequently reverted to basal levels thereafter. These findings indicate that PHIG inhibits IL-12 production of SPE-A-activated monocytes and thereby decreases IFN-g synthesis by T cells and suggest that inhibition of IL-12 and IFN-g production is an important part of the mechanisms underlying PHIG therapy on KD.

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“…Superantigens are a class of bacterially derived substances which stimulate a large fraction of the T cell population without requiring previous sensitization (1)(2)(3)(4)(5)(6)(7)(8). T cell stimulation is mediated by the dual affinity of superantigens for the class II major histocompatibility complex on accessory cells and for the relatively invariant sequence of the variable (V) fi region of the T cell receptor (TCR)' (1)(2)(3)(4)(5)(6)(7)(8).…”

Section: Introductionmentioning

confidence: 99%

“…T cell stimulation is mediated by the dual affinity of superantigens for the class II major histocompatibility complex on accessory cells and for the relatively invariant sequence of the variable (V) fi region of the T cell receptor (TCR)' (1)(2)(3)(4)(5)(6)(7)(8). Depending on the superanti- 1.…”

Section: Introductionmentioning

confidence: 99%

Intravenous immunoglobulin contains specific antibodies inhibitory to activation of T cells by staphylococcal toxin superantigens [see comment]

Takei¹,

Arora²,

Walker³

1993

J. Clin. Invest.

279

143

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Superantigens are products of bacteria with dual affinity for HLA-DR and the variable region of the ft chain of the T cell receptor, leading to the stimulation of large numbers of T cells. Because there is evidence for the involvement of superantigens in various disease conditions in which intravenous IgG (IVIgG) is used as therapy, the purpose of the present study was to determine if IVIgG contains antibodies inhibitory to T cell stimulation by the superantigens. ELISA and Western assays revealed high concentrations of antibodies in the pooled IgG against eight different staphylococcal toxin (Staph-toxin) superantigens. The IVIgG inhibited in vitro stimulation of human peripheral blood T cells by the Staph-toxins, but did not inhibit responses elicited by phytohemagglutinin or anti-CD3. Inhibition was mediated by Staph-toxin-specific antibodies as shown by affinity adsorption depletion studies. The antibodies functioned by inhibiting the binding and/or presentation of Staphtoxins by DR' accessory cells. In conclusion, this report is the first to show that normal pooled IgG contains antibodies against a major group of the superantigens, the Staph-toxins, and that the antibodies can inhibit Staph-toxin-elicited T cell activation, suggesting a possible immunoregulatory role for the antibodies in vivo. (J. Clin. Invest. 1993. 91:602-607.)

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Direct binding of secreted T-cell receptor beta chain to superantigen associated with class II major histocompatibility complex protein.

Cited by 77 publications

References 36 publications

The Decreased Replicative Capacity of Simian Immunodeficiency Virus SIVmac239ΔnefIs Manifest in Cultures of Immature Dendritic Cells and T Cells

The Decreased Replicative Capacity of Simian Immunodeficiency Virus SIVmac239ΔnefIs Manifest in Cultures of Immature Dendritic Cells and T Cells

Inhibition of IL-12 synthesis of peripheral blood mononuclear cells (PBMC) stimulated with a bacterial superantigen by pooled human immunoglobulin: implications for its effect on Kawasaki disease (KD)

Intravenous immunoglobulin contains specific antibodies inhibitory to activation of T cells by staphylococcal toxin superantigens [see comment]

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