Two Kdo-Heptose Regions Identified in<i>Hafnia alvei</i>32 Lipopolysaccharide: the Complete Core Structure and Serological Screening of Different<i>Hafnia</i>O Serotypes

Łukasiewicz, Jolanta; Niedziela, Tomasz; Jachymek, Wojciech; Kenne, Lennart; Ługowski, Czesław

doi:10.1128/jb.00891-08

Cited by 13 publications

(7 citation statements)

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“…Recently we have determined the structure of the carbohydrate backbone of H. alvei 32 lipid A as a part of N,O-deacylated LPS, which is built of the bisphosphorylated disaccharide, Glc p N4P-(1 → 6)-Glc p N1P ( 7 ). We now report on the structural studies of H. alvei ence of ( R )-14:0(3-OH), 14:0, 12:0, and 16:0 as ester-bound fatty acids and ( R )-14:0(3-OH) as the only amide-linked fatty acid.…”

Section: Analytical Proceduresmentioning

confidence: 99%

Structural analysis of the lipid A isolated from Hafnia alvei 32 and PCM 1192 lipopolysaccharides

Łukasiewicz

Jachymek

Niedziela

et al. 2010

Journal of Lipid Research

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Section: Analytical Proceduresmentioning

confidence: 99%

Structural analysis of the lipid A isolated from Hafnia alvei 32 and PCM 1192 lipopolysaccharides

Łukasiewicz

Jachymek

Niedziela

et al. 2010

Journal of Lipid Research

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“…A few strains of H. alvei synthesize LPS containing E. coli R4 [strains Polish Collection of Microorganisms (PCM) 23 or 1222] or Salmonella Ra (strain PCM 1212) core types (Figure 1 ) ( 25 , 26 ). The OS1 hexasaccharide is the predominant core OS for this species, with Hep and Kdo residues in its inner core region like most Gram-negative bacteria (Table 1 , footnote f) ( 24 , 27 ).…”

Section: Introductionmentioning

confidence: 96%

“…So far, 40 O-serotypes (O-PS structures), and 4 types of core OS have been identified. H. alvei LPS is also an example of endotoxin having the E. coli -type structure of lipid A ( 22 – 24 ). A few strains of H. alvei synthesize LPS containing E. coli R4 [strains Polish Collection of Microorganisms (PCM) 23 or 1222] or Salmonella Ra (strain PCM 1212) core types (Figure 1 ) ( 25 , 26 ).…”

Section: Introductionmentioning

confidence: 99%

“… c l -α- d -Hepp3OAc-(1→4)-α-Kdop ( 10 , 23 , 29 ) . d l -α- d -Hepp-(1→4)-[α- d -Galp6OAc-(1→7)]-α-Kdop ( 24 ) . e α- d -Galp-(1→2)- l -α- d -Hepp-(1→4)-α-Kdop ( 30 ) .…”

Section: Introductionunclassified

“…A peculiarity of H. alvei LPS is the presence of Hep-Kdo-containing motifs also in the outer core region ( 24 ) (Figure 1 ). Branched trisaccharide (TS1), l -α- d -Hep p -(1→4)-[α- d -Gal p 6OAc-(1→7)]-α-Kdo p -(2→, was identified, for example, at the outer core region of H. alvei 32 and PCM 1192 LPS (OS1-TS1 core) ( 24 , 31 ). Linear trisaccharide α- d -Gal p -(1→2)- l -α- d -Hep p -(1→4)-α-Kdo p -(2→ (TS2) is characteristic for H. alvei PCM 1196 (OS1-TS2 core) ( 32 ).…”

Section: Introductionmentioning

confidence: 99%

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Interaction of Mannose-Binding Lectin With Lipopolysaccharide Outer Core Region and Its Biological Consequences

et al. 2018

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Lipopolysaccharide (LPS, endotoxin), the main surface antigen and virulence factor of Gram-negative bacteria, is composed of lipid A, core oligosaccharide, and O-specific polysaccharide (O-PS) regions. Each LPS region is capable of complement activation. We have demonstrated that LPS of Hafnia alvei, an opportunistic human pathogen, reacts strongly with human and murine mannose-binding lectins (MBLs). Moreover, MBL–LPS interactions were detected for the majority of other Gram-negative species investigated. H. alvei was used as a model pathogen to investigate the biological consequences of these interactions. The core oligosaccharide region of H. alvei LPS was identified as the main target for human and murine MBL, especially l-glycero-d-manno-heptose (Hep) and N-acetyl-d-glucosamine (GlcNAc) residues within the outer core region. MBL-binding motifs of LPS are accessible to MBL on the surface of bacterial cells and LPS aggregates. Generally, the accessibility of outer core structures for interaction with MBL is highest during the lag phase of bacterial growth. The LPS core oligosaccharide–MBL interactions led to complement activation and also induced an anaphylactoid shock in mice. Unlike Klebsiella pneumoniae O3 LPS, robust lectin pathway activation of H. alvei LPS in vivo was mainly the result of outer core recognition by MBL; involvement of the O-PS is not necessary for anaphylactoid shock induction. Our results contribute to a better understanding of MBL–LPS interaction and may support development of therapeutic strategies against sepsis based on complement inhibition.

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Analysis of carbohydrates and glycoconjugates by matrix‐assisted laser desorption/ionization mass spectrometry: An update for 2009–2010

Harvey

2014

Mass Spectrometry Reviews

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This review is the sixth update of the original article published in 1999 on the application of MALDI mass spectrometry to the analysis of carbohydrates and glycoconjugates and brings coverage of the literature to the end of 2010. General aspects such as theory of the MALDI process, matrices, derivatization, MALDI imaging, arrays and fragmentation are covered in the first part of the review and applications to various structural typed constitutes the remainder. The main groups of compound that are discussed in this section are oligo and polysaccharides, glycoproteins, glycolipids, glycosides and biopharmaceuticals. Many of these applications are presented in tabular form. Also discussed are medical and industrial applications of the technique, studies of enzyme reactions and applications to chemical synthesis.

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Two Kdo-Heptose Regions Identified inHafnia alvei32 Lipopolysaccharide: the Complete Core Structure and Serological Screening of DifferentHafniaO Serotypes

Cited by 13 publications

References 57 publications

Structural analysis of the lipid A isolated from Hafnia alvei 32 and PCM 1192 lipopolysaccharides

Structural analysis of the lipid A isolated from Hafnia alvei 32 and PCM 1192 lipopolysaccharides

Interaction of Mannose-Binding Lectin With Lipopolysaccharide Outer Core Region and Its Biological Consequences

Analysis of carbohydrates and glycoconjugates by matrix‐assisted laser desorption/ionization mass spectrometry: An update for 2009–2010

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