Structural analysis of the lipid A isolated from Hafnia alvei 32 and PCM 1192 lipopolysaccharides

Łukasiewicz, Jolanta; Jachymek, Wojciech; Niedziela, Tomasz; Kenne, Lennart; Ługowski, Czesław

doi:10.1194/jlr.m001362

Cited by 34 publications

(79 citation statements)

References 39 publications

(41 reference statements)

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“…The recovered Lipid A in present study disagreed with that reported by Lukasiewicz and coworkers (12) found the recovered amount was 11-16% of LPS. This differentiation may be due to, structural analysis of lipid A within individual organisms resulting from different growth conditions (e.g.…”

Section: Resultscontrasting

confidence: 57%

Assessment of the Partial Purified Lipid a Extracted from Proteus mirabiliscell Wall as Adjuvant Immunotherapy

2017

IJSR

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P.mirabilis is considered one of the nosocomial infection causative agent, and its importance is related with the wide separation in different ecosystem, in addition to its relationship with renal and bile calculi ,and because of an increase of bacterial resistance, this study goal to find substitutional substance used to treat bacterial infection through using natural biomaterial, thus using LPS (Lipid A moiety) as adjuvant immunotherapy by different routes of administration(Oral, intraperitoneal, and subcutaneous) and find the best one. The results showed, recovered lipid A was obtained from both collected LPS of pathogenic P.mirabilis isolate and standard bacteria approximately represented 20% of LPS amount, with LD50 0.6 ϻg/ml for the pathogenic one. The estimation of some immune parameters such as IL-1β showed i.p administration was led to a significant elevation of this interleukin in compared with other routes and control groups. Meanwhile, IL-2, IL-10, and IFN-γ percentages showed a significant elevation in all treated groups in compared with control. While TNF-α percentage revealed variation in levels according to the route of administration, simultaneously both intraperitoneal and subcutaneous routes showed a significant elevation. Whereas the IgG level exhibited, no significant alteration observed with any treated groups in compared with control one.

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Section: Resultscontrasting

confidence: 57%

Assessment of the Partial Purified Lipid a Extracted from Proteus mirabiliscell Wall as Adjuvant Immunotherapy

2017

IJSR

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“…1A, bacterial endotoxin adopts a complex chemical structure, starting from O-antigen, connected through core-oligosaccharide and ending with lipid A in the cell interior, where lipid A is believed to be responsible for a majority of the biological activities [7,28]. Endotoxin or LPS has the polysaccharide chain with various units of hexose, N -acetyl-hexosamine, heptose, deoxyoctonic acid, phosphate and amine structure as well as 4–6 fatty acid chains with 12–14 carbons.…”

Section: Resultsmentioning

confidence: 99%

“…For example, Caroff et al reported direct micro-extraction and analysis of intact LPS by combined thin-layer chromatography and matrix-assisted laser desorption ionization (MALDI) MS [27]. ESI-MS n have been used for the analysis of lipid A isolated from LPS as well [28]. These studies were mainly focused on the structural characterization of intact or partial LPS, but the convenience of traditional ion sources (such as ESI, MALDI) are limited by their speed, sample status (gas, liquid and solid) or sample introduction conditions (in matrix or/and under vacuum).…”

Section: Introductionmentioning

confidence: 99%

Rapid detection of bacterial endotoxins in ophthalmic viscosurgical device materials by direct analysis in real time mass spectrometry

Hitchins

Wickramasekara

2016

Analytica Chimica Acta

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Bacterial endotoxins are lipopolysaccharides bound to the bacterial cell wall and released when bacteria rupture or disintegrate. Possible contamination of endotoxin in ophthalmic devices can cause a painful eye inflammation or result in toxic anterior segment syndrome after cataract surgery. Measurement of bacterial endotoxin in medical device materials is difficult since endotoxin binds with polymer matrix and some of the materials are very viscous and non-water soluble, where traditional enzyme-based Limulus amebocyte lysate (LAL) assay cannot be applied. Here we propose a rapid and high throughput ambient ionization mass spectrometric (MS) method using direct analysis in real time (DART) for the evaluation of endotoxin contamination in medical device materials. Large and structurally complex endotoxin instantaneously breaks down into low-mass characteristic fragment ions using DART and is detected by MS in both positive and negative ion modes. This method enables the identification and separation of endotoxin from medical materials with a detection limit of 0.03 ng mL−1 endotoxins in aqueous solution. Ophthalmic viscosurgical device materials including sodium hyaluronate (NaHA), non-water soluble perfluoro-n-octane (PFO) and silicone oil (SO) were spiked with different known concentrations of endotoxin and analyzed by DART MS, where the presence of endotoxin was successfully detected and featured small mass fragment ions were generated for NaHA, PFO and SO as well. Current findings showed the feasibility of measuring endotoxin contamination in medical device materials using DART-MS, which can lead to a one-step analysis of endotoxins in different matrices, avoiding any potential contamination during sample pre-treatment steps.

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“…To the best of our knowledge this structural feature was already reported for several strains of Klebsiella pneumoniae, 37 Rhizobium etli 38 and recently for Hafnia alvei. 39 Moreover, in some strains of K. pneumoniae and R. Etli, the outer core a-Kdo connects the O-chain polysaccharide to the core region. 40,41 In particular, for a number of K. pneumoniae strains, it has been found that the GlcN is substituted at the O-6 position of the Kdo residue.…”

Section: Discussionmentioning

confidence: 99%

The complete structure of the core of the LPS from Plesiomonas shigelloides 302–73 and the identification of its O-antigen biological repeating unit

Pieretti

Carillo

Lindner

et al. 2010

Carbohydrate Research

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Structural analysis of the lipid A isolated from Hafnia alvei 32 and PCM 1192 lipopolysaccharides

Cited by 34 publications

References 39 publications

Assessment of the Partial Purified Lipid a Extracted from Proteus mirabiliscell Wall as Adjuvant Immunotherapy

Assessment of the Partial Purified Lipid a Extracted from Proteus mirabiliscell Wall as Adjuvant Immunotherapy

Rapid detection of bacterial endotoxins in ophthalmic viscosurgical device materials by direct analysis in real time mass spectrometry

The complete structure of the core of the LPS from Plesiomonas shigelloides 302–73 and the identification of its O-antigen biological repeating unit

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