Two-Dimensional MoS<sub>2</sub>-Based Electrochemical Biosensor for Highly Selective Detection of Glutathione

Rawat, Brajesh; Mishra, Kundan Kumar; Barman, Ujjwol; Arora, Leena; Pal, Durba; Paily, Roy

doi:10.1109/jsen.2020.2978275

Cited by 30 publications

(14 citation statements)

References 29 publications

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“…However, toxicity studies of MnO 2 nanosheets in the biological environment are still in the preliminary stage 25 , 26 . Although previous reports on GSH sensing using fluorescence methods were claimed as cost-effective and highly sensitive, however, those methods involve complicated experimental procedures and are time-consuming 27 – 29 .…”

Section: Introductionmentioning

confidence: 99%

Integrating gold nanoclusters, folic acid and reduced graphene oxide for nanosensing of glutathione based on “turn-off” fluorescence

Wong

Quesada-González²,

Manickam

et al. 2021

Sci Rep

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Glutathione (GSH) is a useful biomarker in the development, diagnosis and treatment of cancer. However, most of the reported GSH biosensors are expensive, time-consuming and often require complex sample treatment, which limit its biological applications. Herein, a nanobiosensor for the detection of GSH using folic acid-functionalized reduced graphene oxide-modified BSA gold nanoclusters (FA-rGO-BSA/AuNCs) based on the fluorescence quenching interactions is presented. Firstly, a facile and optimized protocol for the fabrication of BSA/AuNCs is developed. Functionalization of rGO with folic acid is performed using EDC/NHS cross-linking reagents, and their interaction after loading with BSA/AuNCs is demonstrated. The formation of FA-rGO, BSA/AuNCs and FA-rGO-BSA/AuNCs are confirmed by the state-of-art characterization techniques. Finally, a fluorescence turn-off sensing strategy is developed using the as-synthesized FA-rGO-BSA/AuNCs for the detection of GSH. The nanobiosensor revealed an excellent sensing performance for the detection of GSH with high sensitivity and desirable selectivity over other potential interfering species. The fluorescence quenching is linearly proportional to the concentration of GSH between 0 and 1.75 µM, with a limit of detection of 0.1 µM under the physiological pH conditions (pH 7.4). Such a sensitive nanobiosensor paves the way to fabricate a “turn-on” or “turn-off” fluorescent sensor for important biomarkers in cancer cells, presenting potential nanotheranostic applications in biological detection and clinical diagnosis.

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Section: Introductionmentioning

confidence: 99%

Integrating gold nanoclusters, folic acid and reduced graphene oxide for nanosensing of glutathione based on “turn-off” fluorescence

Wong

Quesada-González²,

Manickam

et al. 2021

Sci Rep

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“…The sensor had a very broad linear range, which spanned from micromolar to millimolar with the detection limit in nanomolar. 127 A composite of cobalt phthalocyanine and functionalized multiwalled carbon nanotubes was fabricated for detection of glutathione. The LOD value was found to be 100 μM and 8.3 μM for reduced glutathione and oxidized glutathione respectively.…”

Section: D Layered Materials Based (Bio)sensors For Oxidative Stress ...mentioning

confidence: 99%

Advancements in 2D Materials Based Biosensors for Oxidative Stress Biomarkers

Garg

Gupta

Sharma

et al. 2021

ACS Appl. Bio Mater.

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The imbalance in generation of reactive oxygen species and its depletion causes oxidative stress. Because of its importance, there is a need to explore the role of oxidative stress biomarkers. The limitations of the conventional methods cause the researchers look for other alternatives. Biosensors are highly promising candidates for the detection of trace quantities for various analytes with high specificity, selectivity, and quick response time. Nanomaterial based matrices are the most popular choice while fabricating a biosensor. Two-dimensional (2D) materials such as graphene, transitional metal dichalcogenides, and various metal oxides have been used for the biosensing of different oxidative stress analytes. High electron mobility, good optical properties, tunable properties, high yields, easy synthesis, and availability make these materials the first choice. In this review, we have comprehensively discussed various biomarkers associated with oxidative stress. The review will provide information related to different kinds and various synthesis procedures employed for 2D nanomaterials. The major focus of the review is to elaborate the role of 2D nanomaterial based structures for the optical and electrochemical methods for the detection of oxidative stress. This review is an effort to help the researchers better understand various 2D based transducers available for the detection of oxidative stress biomarkers.

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“…A new electrochemical biosensor based on GST immobilized on multilayer MoS 2 (molybdenum disulphide) nanosheet, based on GSH and CDNB conjugation reaction, was developed [84]. Unlike the previous device, it exhibited satisfactory repeatability and stability that encourages its use for cancer diagnostics.…”

Section: Gst and Non‐gst Based Biosensors For The Detection And Quantification Of Gshmentioning

confidence: 99%

“…Thus, the importance of monitoring GSH concentrations in physiological systems for medical diagnosis is clear. Several different biosensors have been developed for the detection and quantification of GSH including electrochemical, FRETand photoelectrochemical (PEC)-based biosensors [84][85][86][87][88][89][90].…”

Section: Gst and Non-gst Based Biosensors For The Detection And Quant...mentioning

confidence: 99%

An Overview of Biosensors Based on Glutathione Transferases and for the Detection of Glutathione

2021

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Glutathione transferases are enzymes involved in the detoxification against xenobiotics and noxious compounds. These enzymes catalyse a variety of reactions on many physiological and xenobiotic compounds using glutathione as a co-substrate. Moreover, many compounds are inhibitors of such enzymes. A wide array of biosensors based on glutathione transferases have been developed for analysing a variety of noxious compounds, as well as several biosensors devoted to the detection and quantification of glutathione and of glutathione transferases themselves. Here, we review the state of the art in this active field of research, highlighting the possible applications of such devices.

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Two-Dimensional MoS₂-Based Electrochemical Biosensor for Highly Selective Detection of Glutathione

Cited by 30 publications

References 29 publications

Integrating gold nanoclusters, folic acid and reduced graphene oxide for nanosensing of glutathione based on “turn-off” fluorescence

Integrating gold nanoclusters, folic acid and reduced graphene oxide for nanosensing of glutathione based on “turn-off” fluorescence

Advancements in 2D Materials Based Biosensors for Oxidative Stress Biomarkers

An Overview of Biosensors Based on Glutathione Transferases and for the Detection of Glutathione

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Two-Dimensional MoS2-Based Electrochemical Biosensor for Highly Selective Detection of Glutathione

Cited by 30 publications

References 29 publications

Integrating gold nanoclusters, folic acid and reduced graphene oxide for nanosensing of glutathione based on “turn-off” fluorescence

Integrating gold nanoclusters, folic acid and reduced graphene oxide for nanosensing of glutathione based on “turn-off” fluorescence

Advancements in 2D Materials Based Biosensors for Oxidative Stress Biomarkers

An Overview of Biosensors Based on Glutathione Transferases and for the Detection of Glutathione

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Two-Dimensional MoS₂-Based Electrochemical Biosensor for Highly Selective Detection of Glutathione