Metal–organic frameworks (MOFs)
are envisaged as highly
useful for the development of biosensors. Herein, for the first time,
we report the optical detection of Escherichia coli using a water-dispersible terbium MOF (Tb-BTC; BTC, 1,3,5-benzenetricarboxylic
acid). The successful synthesis of Tb-BTC is verified using spectroscopic
and morphological techniques like UV–vis, fluorescence and
FTIR spectroscopy, X-ray diffraction analysis, and electron microscopy.
Tb-BTC has been bio-interfaced with anti-E. coli antibodies and then investigated as a biosensor for E. coli. The biosensor displays detection ability
in an analyte concentration range of 1.3 × 102 to
1.3 × 108 cfu/mL with a detection limit of 3 cfu/mL,
having a response time of 5 min and a total analysis time of about
20–25 min. The results are also found to be reproducible and
specific in the presence of some other interfering bacterial species.
As demonstrated, the present sensor provides highly sensitive and
specific detection of E. coli in fruit
juice sample. To the best of our knowledge, this is the first report
to showcase the potential of the MOF-based fluorescent biosensor for
the detection of E. coli.
A Cu-MOF/PANI modified screen-printed electrode based immunosensing technique is described for the sensitive detection of cardiac troponin I. The sensor provides detection over a wide concentration range with a limit of detection of 0.8 ng mL−1.
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