Two classes of mouse mast cells delineated by monoclonal antibodies.

Katz, Howard R.; LeBlanc, Paul A.; Russell, Stephen W.

doi:10.1073/pnas.80.19.5916

Cited by 39 publications

(18 citation statements)

References 20 publications

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“…Moreover, peritoneal mast cells are typical connective tissue mast cells, whereas mast ceil-like cells in the suspension culture are considered to resemble mucosal mast cells (47). Biochemical, functional, and immunological characterization revealed the difference between connective tissue mast cells and mucosal mast cells (47,48). There is a possibility that the peritoneal mast cell with proliferative potentiality may have characteristics similar to mast cell-like cells that developed in vitro.…”

Section: Discussionmentioning

confidence: 99%

“…However, mitosis of peritoneal mast cells is seldom observed in intact animals (10). Moreover, peritoneal mast cells are typical connective tissue mast cells, whereas mast ceil-like cells in the suspension culture are considered to resemble mucosal mast cells (47). Biochemical, functional, and immunological characterization revealed the difference between connective tissue mast cells and mucosal mast cells (47,48).…”

Section: Discussionmentioning

confidence: 99%

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Proliferation of peritoneal mast cells in the skin of W/Wv mice that genetically lack mast cells.

Sonoda¹,

Kanayama²,

Hara³

et al. 1984

The Journal of Experimental Medicine

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Presence of mast cell precursors in the mouse peritoneal cavity was demonstrated, and the precursors were characterized. When a cell suspension, containing mast cell precursor(s), was directly injected into the skin of genetically mast cell-deficient WBB6F1 (WB X C57BL/6)-W/Wv mice, a cluster composed of approximately 2,000 mast cells appeared at the injection site. By determining the proportion of injection sites at which the mast cell cluster appeared, the concentration of mast cell precursors can be calculated by limiting dilution analysis. The concentration in the peritoneal cavity was about five times as great as the concentration in the bone marrow. Although peritoneal mast cell precursors were shown to originate from the bone marrow, physical characterization revealed that the peritoneal precursors differed from the marrow precursors. The peritoneal precursors were less susceptible to irradiation than the marrow precursors; the former were heavier than the latter. When a 95% pure mast cell suspension was prepared from the peritoneal cells by the removal of phagocytes and the density gradient centrifugation, 1 out of 16 cells had the potentiality to make a mast cell cluster in the skin of the W/Wv mice. Moreover, when a single mast cell was identified under the phase contrast microscope and picked up with the micromanipulator, 1 out of 17 mast cells made the cluster. This indicated that some peritoneal mast cells kept extensive proliferative potentiality even after morphological differentiation. In other words, some peritoneal mast cells themselves may function as the committed precursors.

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Section: Discussionmentioning

confidence: 99%

Section: Discussionmentioning

confidence: 99%

Proliferation of peritoneal mast cells in the skin of W/Wv mice that genetically lack mast cells.

Sonoda¹,

Kanayama²,

Hara³

et al. 1984

The Journal of Experimental Medicine

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“…It therefore will be important to use direct biochemical methods to confirm the presence of heparin in the mast cells. Evaluations of other aspects of the phenotype of the recovered cells, such as expression of membrane structures characteristic of cultured as opposed to normal peritoneal mast cells (10,54) will also be of interest.…”

Section: Discussionmentioning

confidence: 99%

Fate of bone marrow-derived cultured mast cells after intracutaneous, intraperitoneal, and intravenous transfer into genetically mast cell-deficient W/Wv mice. Evidence that cultured mast cells can give rise to both connective tissue type and mucosal mast cells.

Nakano¹,

Sonoda²,

Hayashi³

et al. 1985

The Journal of Experimental Medicine

471

268

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Both connective tissue mast cells and mast cells grown in vitro are derived from multipotential hematopoietic stem cells, but these two mast cell populations exhibit many differences in morphology, biochemistry, and function. We investigated whether the phenotype of cultured mast cells or their progeny was altered when the cells were transferred into different locations in vivo. Cultured mast cells were immature by ultrastructure, and stained with alcian blue but with neither safranin or berberine sulfate, a fluorescent dye that binds to the heparin of connective tissue mast cell granules. By contrast, mast cells recovered from the peritoneal cavity of congenitally mast cell-deficient (WB X C57BL/6)F1-W/Wv (WBB6F1-W/Wv) mice 10 wk after intraperitoneal injection of cultured WBB6F1-+/+ or C57BL/6-bgJ/bgJ mast cells stained with both safranin and berberine sulfate. Staining with berberine sulfate was prevented by treatment of the cells with heparinase but not chondroitinase ABC, suggesting that the adoptively transferred mast cell population had acquired the ability to synthesize and store heparin. Furthermore, the recovered mast cells were indistinguishable by ultrastructure from the normal mature peritoneal mast cells of WBB6F1-+/+ mice, and contained substantially more histamine than mast cells studied directly from culture. Intravenous injection of cultured mast cells resulted in the development of safranin-and berberine sulfate-positive mast cells in the peritoneal cavity, spleen, skin, and glandular stomach muscularis propria. Mast cells also developed on the glandular stomach mucosa, but these cells stained with alcian blue rather than safranin, and did not stain with berberine sulfate. This result suggests that cultured mast cells can give rise to mast cells of either the connective tissue type or mucosal phenotype, depending on anatomical location. Furthermore, transplantation of cultured mast cells into WBB6F1-W/Wv mice had no measurable effect on the anemia of the recipient mice, suggesting a possible strategy for repairing the mast cell deficiency of WBB6F1-W/Wv mice without affecting other bone marrow-derived populations such as erythrocytes. Intravenous injection of representative connective tissue type mast cells (30-50% pure peritoneal mast cells derived from WBB6F1-+/+ mice) gave results similar to those obtained with cultured mast cells: mast cells developing in the peritoneal cavity, skin, spleen, and glandular stomach muscularis propria of WBB6F1-W/Wv recipients stained with safranin and berberine sulfate, whereas mast cells developing in the mucosa of the glandular stomach stained only with alcian blue.(ABSTRACT TRUNCATED AT 400 WORDS)

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“…Murine gp49 molecules are 49-kDa Ig-like receptors expressed on the surfaces of cells involved in innate and acquired immunity such as NK cells, mast cells, neutrophils, macrophages, and activated T cells [12,24,25]. The two major subtypes, gp49A and gp49B, are encoded by two different genes adjacent to each other [12,26,27].…”

Section: Introductionmentioning

confidence: 99%

“…In addition, overexpression of inhibitory receptors such as leukocyte Ig-like receptor (LILR)B2 or LILRB4 (also known as ILT4/LIR-2/MIR-10/CD85d or ILT3/LIR-5/ HM18/CD85k, respectively) reduces T-cell activation [7]. Although some of the functions of these receptors on DC in T-cell responses have been determined, the roles of many others in the physiologic regulation of DC as well as of immune responses remain unclear.Murine gp49 molecules are 49-kDa Ig-like receptors expressed on the surfaces of cells involved in innate and acquired immunity such as NK cells, mast cells, neutrophils, macrophages, and activated T cells [12,24,25]. The two major subtypes, gp49A and gp49B, are encoded by two different genes adjacent to each other [12,26,27].…”

mentioning

confidence: 99%

A novel regulatory role of gp49B on dendritic cells in T‐cell priming

et al. 2008

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Dendritic cells (DC) play pivotal roles in the induction and regulation of both innate and acquired immunity. DC express several cell-surface immune inhibitory receptors. However, little is known about their potential immunoregulatory functions in the context of T-cell activation. Here we report that murine gp49B, a member of the immunoglobulin superfamily, harboring immunoreceptor tyrosine-based inhibitory motifs, is expressed on DC and downregulates cellular activity to prevent the excessive activation of T cells in vitro and in vivo. Bone marrow-derived DC (BMDC) from newly generated gp49B-deficient (gp49B À/À ) mice induced enhanced proliferation and IL-2 release of antigen-specific CD4 1 and CD8 1 T cells compared with BMDC from wild-type mice, in a cell-cell contact manner. The enhanced proliferation by gp49B À/À BMDC was also observed in allogeneic CD4 1 and CD8 1 T cells. Moreover, the transfer of allogeneic BALB/c splenocytes into C57BL/6 gp49B À/À mice induced severe acute graft-versus-host disease with an augmented upregulation of CD86 on CD11c 1 splenic gp49B À/À DC, while transfer of C57BL/6 gp49B À/À splenocytes into BALB/c mice did not, suggesting the exacerbation of the disease was due, at least in part, to augmented activation of recipient gp49B À/À DC. These findings demonstrate a novel regulatory role of gp49B in the function of DC.Key words: Antigen presentation Á Costimulation Á DC Á Regulatory receptor Á Tolerance IntroductionDendritic cells (DC) are professional antigen-presenting cells that recognize a diverse repertoire of antigens and present them to naïve T cells for the induction of acquired immunity [1]. DC are also related to innate immunity by producing cytokines such as both type I and II IFN, or by activating NK cells [2,3]. In addition, recent reports revealed that a subset of DC maintains peripheral tolerance in the steady state [4], showing that DC play a pivotal role in the induction of immune responses including autoimmunity.On the DC surface, a variety of structurally different immunoreceptors that regulate DC and T-cell functions have been found, including CD80, CD86, CD40, and various Ig superfamily and lectin family molecules [5]. Among them, a group of paired Ig superfamily receptors, termed Ig-like receptors, have been attracting the interest of many researchers because they are expressed on various subsets of DC and are considered to contribute to the development or activation of DC [6][7][8][9][10]. Generally, Ig-like receptors are subdivided into two types, namely activating and inhibitory receptors, and in some cases they are expressed on hematopoietic cells in a pair-wise manner [11][12][13][14]. Most activating receptors associate with homodimeric subunits containing ITAM, such as the FcR common g chain and KARAP/DAP12 [15,16], and deliver positive signals into the cells through interaction with their ligands [17]. For instance, the uptake of immune complexes through activating FcR for IgG 2426augments DC maturation, leading to efficient antigen presentation [18...

show abstract

Two classes of mouse mast cells delineated by monoclonal antibodies.

Cited by 39 publications

References 20 publications

Proliferation of peritoneal mast cells in the skin of W/Wv mice that genetically lack mast cells.

Proliferation of peritoneal mast cells in the skin of W/Wv mice that genetically lack mast cells.

Fate of bone marrow-derived cultured mast cells after intracutaneous, intraperitoneal, and intravenous transfer into genetically mast cell-deficient W/Wv mice. Evidence that cultured mast cells can give rise to both connective tissue type and mucosal mast cells.

A novel regulatory role of gp49B on dendritic cells in T‐cell priming

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