Proliferation of peritoneal mast cells in the skin of W/Wv mice that genetically lack mast cells.

Abstract

Presence of mast cell precursors in the mouse peritoneal cavity was demonstrated, and the precursors were characterized. When a cell suspension, containing mast cell precursor(s), was directly injected into the skin of genetically mast cell-deficient WBB6F1 (WB X C57BL/6)-W/Wv mice, a cluster composed of approximately 2,000 mast cells appeared at the injection site. By determining the proportion of injection sites at which the mast cell cluster appeared, the concentration of mast cell precursors can be calculated by limiting dilution analysis. The concentration in the peritoneal cavity was about five times as great as the concentration in the bone marrow. Although peritoneal mast cell precursors were shown to originate from the bone marrow, physical characterization revealed that the peritoneal precursors differed from the marrow precursors. The peritoneal precursors were less susceptible to irradiation than the marrow precursors; the former were heavier than the latter. When a 95% pure mast cell suspension was prepared from the peritoneal cells by the removal of phagocytes and the density gradient centrifugation, 1 out of 16 cells had the potentiality to make a mast cell cluster in the skin of the W/Wv mice. Moreover, when a single mast cell was identified under the phase contrast microscope and picked up with the micromanipulator, 1 out of 17 mast cells made the cluster. This indicated that some peritoneal mast cells kept extensive proliferative potentiality even after morphological differentiation. In other words, some peritoneal mast cells themselves may function as the committed precursors.

Fate of bone marrow-derived cultured mast cells after intracutaneous, intraperitoneal, and intravenous transfer into genetically mast cell-deficient W/Wv mice. Evidence that cultured mast cells can give rise to both connective tissue type and mucosal mast cells.

“…Remarkably, the mast cells in the gastric mucosa stained only with alcian blue. The CFU-S represents a precursor of the mast cell lineage (32,47), but normal mouse peritoneal mast cells can exhibit extensive proliferative potential when injected intracutaneously into WBB6FI-W/W ~ mice (40). Taken together, these observations suggest that the alcian blue-positive mast cells in the gastric mucosa were actually derived from the injected peritoneal mast cells, and raises the possibility that, under certain circumstances, CTMC give rise to cells with at least some of the features of MMC.…”

“…Cultured or peritoneal ceils of WBB6F1-+/+ mouse origin were injected intravenously into syngeneic mice irradiated (850 tad) as previously described (40). The mice were killed 8 d after cell injection; spleens were removed and fixed in Bouin's solution, and colonies were counted at a magnification of 7× with a dissecting microscope.…”

“…The method has been described in detail (40). Briefly, most mouse peritoneal cells are macrophages and small lymphocytes.…”

Stimulation of connective tissue-type mast cell proliferation by crosslinking of cell-bound IgE.

“…Mature CTMC (398% purity) were separated from peritoneal cells by the removal of phagocytes followed by Percoll (Sigma Chemical Co., St . Louis, MO) densitygradient centrifugation as described in detail (10) .…”

Nerve-mast cell and smooth muscle-mast cell interaction mediated by cell adhesion molecule-1, CADM1

“…In addition, while most myeloid cells lose proliferation potential after maturation, peripherally resident mature mast cells still retain proliferation potential (Sonoda et al, 1984). Even after they have executed degranulation, mature mast cells are able not only to proliferate but also to acquire the original morphology by generating cytoplasmic granules (Kuriu et al, 1989;Jozaki et al, 1990).…”