Turning rice meiosis into mitosis

Mieulet, Delphine; Jolivet, Sylvie; Rivard, Maud; Cromer, Laurence; Vernet, Aurore; Mayonove, Pauline; Pereira, Lucie; Droc, Gaëtan; Courtois, B.; Guiderdoni, Emmanuel; Mercier, Raphaël

doi:10.1038/cr.2016.117

Cited by 123 publications

(120 citation statements)

References 51 publications

Supporting

Mentioning

Contrasting

Unclassified

Order By: Relevance

“…We previously got a Cas9‐derived Ososd1 with a frame‐shifted mutation (a G insertion at the 17 nt of the OsOSD1 sgRNA target sequences shown in Figure A). In this Ososd1 mutant, behaviors of chromosomes were normal during meiosis I, but the process was ended at production of dyads without second meiotic division, similar to the reported Ososd1 allele (Figure P–S) (Mieulet et al ).…”

Section: Resultssupporting

confidence: 86%

“…To alter meiosis into mitosis, we mutated OsSPO11-1, OsREC8, and OsOSD1 these three genes and consequently generated a MiMe phenotype. Unlike the reported OsMiMe line (Mieulet et al 2016), we mutated OsSPO11-1 instead of PAIR1, suggesting that any gene essential for double-strand break formation is sufficient to generate MiMe line in rice. In AOP, we edited OsMATL with target site near C termini of the CDS, similar to the mutant sites previously reported (Yao et al 2018), to generate the clonal progeny through a haploid inducing process.…”

Section: Resultsmentioning

confidence: 94%

“…This scheme has first been proved in Arabidopsis thaliana by crossing a triple mutant called MiMe ( Mitosis instead of Meiosis ) with a CENH3‐modified genome elimination line ( GEM ). However, in this system, the MiMe needs to be artificially crossed with the GEM line to produce clonal progeny, and the CENH3‐modified GEM line has not been successfully transferred in other species (Mieulet et al ). Recently, OsMATL have been found responsible for haploid induction ability.…”

Section: Introductionmentioning

confidence: 99%

See 2 more Smart Citations

A strategy for generating rice apomixis by gene editing

Xie

Tang

et al. 2019

JIPB

View full text Add to dashboard Cite

Summary Apomixis is an asexual reproduction way of plants that can produce clonal offspring through seeds. In this study, we introduced apomixis into rice (Oryza sativa) by mutating OsSPO11‐1, OsREC8, OsOSD1, and OsMATL through a CRISPR/Cas9 system. The quadruple mutant showed a transformation from meiosis to mitosis and produced clonal diploid gametes. With mutated Osmatl, which gives rise to haploid induction in plants, the quadruple mutant is expected to be able to be produced apomictic diploid offspring. We named this quadruple mutant as AOP (Apomictic Offspring Producer) for its ability to produce apomictic offspring.

show abstract

Section: Resultssupporting

confidence: 86%

Section: Resultsmentioning

confidence: 94%

Section: Introductionmentioning

confidence: 99%

See 1 more Smart Citation

A strategy for generating rice apomixis by gene editing

Xie

Tang

et al. 2019

JIPB

View full text Add to dashboard Cite

show abstract

“…Previously, an important finding was reported that a simultaneous knock‐down of three genes related to meiosis, SPO11‐1 , REC8, and OSD1 , can turn from the process of meiosis of egg cells to that of mitosis (Mitosis instead of Meiosis; MiMe) in Arabidopsis and rice. Namely, this demonstrated that scientists can induce egg cells carrying diploid genomes by an asexual process (Mieulet et al 2016). Further studies revealed that two genes, such as Babyboom1 ( BBM1 ) and Matrilineal ( MATL ), which are involved in parthenogenesis development of egg cells and haploid induction ability, respectively, are important for asexual development of egg cells in maize and rice (Kelliher et al 2017; Khanday et al 2019).…”

mentioning

confidence: 99%

Is one‐line hybrid rice coming?

Wang

Matsuoka

2019

JIPB

View full text Add to dashboard Cite

show abstract

“…Here we show that heterozygosity of F 1 hybrid rice can be fixed and thus propagated without additional crossing. First, we showed that multiplex editing of three key meiotic genes 5,6 in hybrid rice leads to the production of clonal diploid gametes and tetraploid seeds. Next, editing of the MATRILINEAL ( MTL ) gene that involved in fertilization 7,8 results in the induction of haploid seeds in hybrid rice.…”

mentioning

confidence: 99%

Clonal seeds in hybrid rice using CRISPR/Cas9

Shen

et al. 2018

Preprint

Self Cite

View full text Add to dashboard Cite

12 13 2 Heterosis, the observation that first generation hybrids outcompete the parental 14 lines, is widely used in increasing the productivity and yield of agricultural 15 crops 1,2 . However, heterosis is lost in the following generations because of genetic 16 segregation. In addition, the high cost of hybrid seed production hinders the 17 application of heterosis in many crops. Clonal reproduction through seeds could 18 be revolutionary for agriculture by allowing self-propagation of F1 hybrids 3,4 . 19Here we show that heterozygosity of F1 hybrid rice can be fixed and thus 20 propagated without additional crossing. First, we showed that multiplex editing 21 of three key meiotic genes 5,6 in hybrid rice leads to the production of clonal 22 diploid gametes and tetraploid seeds. Next, editing of the MATRILINEAL (MTL) 23 gene that involved in fertilization 7,8 results in the induction of haploid seeds in 24 hybrid rice. By simultaneous editing of these four endogenous genes in hybrid 25 rice using the CRISPR/Cas9 system, we obtained in one generation plants able to 26 propagate clonally through seeds. This opens the possibility to fix heterozygosity 27 of hybrid varieties in food crops. 28 Heterosis (also known as hybrid vigor) is a phenomenon whereby hybrid 29 offspring of genetically diverse individuals display increased vigor relative to their 30 homozygous parents. Heterosis has been widely applied in agriculture to dramatically 31improve the production and to broaden adaptability of crops 1,2 . However, the essential 32 process of hybrid seed production increases the seed cost and even prohibits its 33 application in many crops. It has been proposed to fix the heterosis of hybrid crop by 34 introduction of apomixis 3 . Apomixis is an asexual reproductive strategy where the 35 3 offspring were generated through seeds, but without meiosis and fertilization. 36 Although it has been described in many flowering plant taxa 9 , apomixis has not been 37 reported in major crops. Previously, it was revealed that combined mutations of three 38 genes that affect key meiotic processes created a genotype called MiMe (Mitosis 39 instead of Meiosis) in which meiosis is totally replaced by mitotic-like division, 40 leading to the production of male and female clonal diploid gametes in Arabidopsis 41 and rice 5,6 . However, the self-fertilization of MiMe resulted in doubling of ploidy at 42 each generation. By crossing Arabidopsis MiMe with CenH3-mediated chromosome 43 elimination line, clonal diploid offspring were obtained 4 . However, the system still 44 relies on the crossing between different plants and the CENH3-mediated chromosome 45 elimination appeared to be difficult to transfer to other species 10 . Therefore, further 46 work is required to achieve the aim of heterosis fixation in self-fertilized hybrids.47

show abstract

Turning rice meiosis into mitosis

Cited by 123 publications

References 51 publications

A strategy for generating rice apomixis by gene editing

A strategy for generating rice apomixis by gene editing

Is one‐line hybrid rice coming?

Clonal seeds in hybrid rice using CRISPR/Cas9

Contact Info

Product

Resources

About