Turn-on Detection of Targeted Biochemical Reactions by Triple Resonance NMR Analysis Using Isotope-labeled Probe

Abstract

We report on a strategy for the "turn-on" detection of target biochemical (metabolic) reactions using a triple resonance NMR technique with an isotope-labeled probe. Our NMR study clearly reveals that otherwise NMR-nonactive-13 C/ 2

Molecular Sensors for NMR-Based Detection

“…This strategy not only effectively monitors the metabolic process of a target molecule but also functions as an on/off-typed sensing scheme if such a 1 H–{ 13 C– 13 C′} sequence is created during a reaction or a biological process (Figure ). This method has been successfully applied to the selective detection of lactate (Figure B), hypochlorite (Figure C), and dopamine metabolism (Figure D) . The specific sequence used in triple resonance NMR is not restricted to 1 H–{ 13 C– 13 C′}.…”

Polymeric 1H MRI Probes for Visualizing Tumor In Vivo

“…These results clearly demonstrated that the perfect selectivity of 1D 1 H–{ 13 C– 15 N} triple–resonance NMR allowed the unambiguous and quantitative metabolic and pharmacokinetic ex vivo analyses of 13 C/ 15 N–enriched probe in mice. More recently, other groups have developed the signal turn–on strategy using a combination of 1 H–{ 13 C– 13 C'} triple–resonance NMR and a 13 C–enriched probe for selective sensing of biochemical reactions, such as glucose–to–lactate metabolism, dopamine oxidation, and reactive oxygen species …”

Atom arrangement strategy for designing a turn-on1H magnetic resonance probe: a dual activatable probe for multimodal detection of hypochlorite

“…Triple resonance is used to correlate three NMR-active nuclei with different Larmor frequencies 8 and has been applied mostly to structural analysis of biopolymers, except for precedents, 9,10 including studies from our laboratory. 10 For example, when the pulse scheme allows the magnetic coherence of 1 H to transfer to two successive 13 C nuclei with different Larmor frequencies ( 13 C and 13 C 0 ) through scalar couplings, only the 1 H in the specific sequence 1 H-13 C-13 C 0 is detectable. Therefore, we hypothesized that if we could design a 13 C-labelled chemical probe, which initially has no detectable 1 H-13 C-13 C 0 atom sequence but develops an active 1 H-13 C-13 C 0 sequence upon reaction with ROS, it should function as an off-to-on-type 1 H probe under triple resonance conditions (Fig.…”