Türkiye’deki Dört Sığır Irkında Büyüme Hormonu Gen Polimorfizmi

Abstract

SummaryThis study was conducted to determine DNA-polymorphism of a AluI RFLP at bovine growth hormone (bGH) gene in Zavot (n=48), East Anatolian Red (n=40), Simmental (n=94) and Brown Swiss (n=64) cattle breeds. A total of 246 cattle were genotyped for the bGH-AluI polymorphism by polymerase chain reaction and restriction fragment length polymorphism (PCR-RFLP). In the study, two alleles (L and V) and three genotypes (LL, VV and LV) were revealed after than digestion of amplification product with AluI restriction enzyme. Allelic frequencies for EAR, SIM, BS and Zavot breeds were determined as 0.775, 0.734, 0.781 and 0.760 respectively for L allele; 0.225, 0.266, 0.219 and 0.240 respectively for V allele. Otherwise, genotypic frequencies were 0.65, 0.57, 0.63 and 0.63 for LL, 0.10, 0.11, 0.06 and 0.10 for VV, and 0.25, 0.32, 0.31 and 0.27 for LV respectively. A significant deviation from Hardy-Weinberg equilibrium was not observed in the investigated breeds. As a result, this study provided information on the polymorphism of bGH in four cattle breeds. Additionally, this study reported the existence of a genetic polymorphism at bGH gene in Zavot cattle breed for the first time. Çalışılan ırklarda Hardy-Weinberg dengesinden sapma görülmemiştir. Sonuç olarak, bu çalışmada dört sığır ırkında bGH gen polimorfizmi hakkında bilgi verilmiştir. Ayrıca, bu çalışma ile Zavot sığırında bGH gen polimorfizmi varlığı ilk defa bildirilmiştir.

Fertil ve Subfertil Siyah Alaca Düveler Arasında CYP19, ERα, PGR Allel Frekanslarının Dağılımı

“…However, the results obtained from these studies were not in concordance with each other. While in some previous studies significant relationships have been found between polymorphisms in the CYP19 and ERα genes and calving to conception intervals [20] , calving difficulties [22,35] and milk production, some of them did not find any associations with economically important traits, such as fertility [13] and milk production [36][37][38] . The gene and genotype frequency distributions for all of the loci did not differ between the fertile and subfertile groups.…”

“…It is also important to determine the genes and genotype frequencies of candidate gene for quantitative traits. Various studies have been performed to determinate the gene and genotype frequencies affecting quantitative traits in cattle breeds reared in Turkey [12,13] .…”

“…Although there are many causative factors, hormonal imbalance, genetic factors and the uterine environment are important as etiological factors in subfertile heifers [7,8] . Genetic markers might be helpful for selecting more appropriate individuals to keep in breeding herds [12,13] .…”

Holstein-Friesian Düvelerde Fertilite ile GH, PRL, STAT5A, OPN, PIT-1, LEP ve FGF2 Polimorfizimlerinin İlişkileri

“…These polymorphisms may be important for the improvement of reproductive traits. Gene regions effect reproductive and productive traits should be also investigated in native breeds to reveal genetic composition of these breeds as some investigation groups have already done [53,54] .…”

Investigation of Polymorphisms on ABCG2, AA-NAT and FABP3 Genes in the Kıvırcık Sheep Reared in Three Different Provinces of Turkey

“…In Turkey lack of production records is a limiting factor to analyze this kind of associations. On the other hand investigations of allelic frequencies of economically important traits loci have been increased among Turkish native livestock species as cattle, goat and sheep, in recent years [4][5][6] . It is assumed that mutations occur on ABCG2, AA-NAT and FABP3 genes have effects on some economically important traits and can be thought as molecular markers.…”