Tumorzellnachweis in Knochenmark, peripherem 
Blut und Leukaphereseprodukten bei Patientinnen mit Mammakarzinom

Xy, Zhong

doi:10.1055/s-2000-7012

Cited by 2 publications

(1 citation statement)

References 16 publications

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“…This step is important for patients faced with the spread of disseminated tumor cells after resection of the solid tumor. 16 Furthermore, some markers used by CellSearch like CK19, EGF receptor, and mammaglobin have been shown to be expressed in normal mitogen-stimulated peripheral blood mononuclear cells. 17 An alternative paradigm for CTC detection is through leveraging differences in physical characteristics of tumor cells, such as size, charge, density, and biomarker expression which can ultimately lead to target-cell isolation.…”

Section: Introductionmentioning

confidence: 99%

Separation of tumor cells with dielectrophoresis-based microfluidic chip

et al. 2013

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The present work demonstrates the use of a dielectrophoretic lab-on-a-chip device in effectively separating different cancer cells of epithelial origin for application in circulating tumor cell (CTC) identification. This study uses dielectrophoresis (DEP) to distinguish and separate MCF-7 human breast cancer cells from HCT-116 colorectal cancer cells. The DEP responses for each cell type were measured against AC electrical frequency changes in solutions of varying conductivities. Increasing the conductivity of the suspension directly correlated with an increasing frequency value for the first cross-over (no DEP force) point in the DEP spectra. Differences in the cross-over frequency for each cell type were leveraged to determine a frequency at which the two types of cell could be separated through DEP forces. Under a particular medium conductivity, different types of cells could have different DEP behaviors in a very narrow AC frequency band, demonstrating a high specificity of DEP. Using a microfluidic DEP sorter with optically transparent electrodes, MCF-7 and HCT-116 cells were successfully separated from each other under a 3.2 MHz frequency in a 0.1X PBS solution. Further experiments were conducted to characterize the separation efficiency (enrichment factor) by changing experimental parameters (AC frequency, voltage, and flow rate). This work has shown the high specificity of the described DEP cell sorter for distinguishing cells with similar characteristics for potential diagnostic applications through CTC enrichment. V C 2013 American Institute of Physics. [http://dx

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Section: Introductionmentioning

confidence: 99%