Separation of tumor cells with dielectrophoresis-based microfluidic chip

AlShareef, Mohammed R.; Metrakos, Nicholas; Perez, Eva Juarez; Azer, Fadi; Yang, Fang; Yang, Xiaoming; Wang, Guiren

doi:10.1063/1.4774312

Cited by 164 publications

(139 citation statements)

References 43 publications

(42 reference statements)

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“…[4][5][6][7] Electrode based DEP (eDEP) technique is normally used to generate non-uniform electric fields in the channel. 8,9 Micro-patterned electrodes in the channel generate highly localized electric fields and trapping is observed to be concentrated around the electrodes. Insulator based DEP (iDEP), on the other hand, uses insulating structures rather than embedded electrodes to a) D. Nakidde, P. Zellner, and M. M. Alemi contributed equally to this work.…”

Section: Introductionmentioning

confidence: 99%

Three dimensional passivated-electrode insulator-based dielectrophoresis

et al. 2015

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In this study, a 3D passivated-electrode, insulator-based dielectrophoresis microchip (3D pDEP) is presented. This technology combines the benefits of electrodebased DEP, insulator-based DEP, and three dimensional insulating features with the goal of improving trapping efficiency of biological species at low applied signals and fostering wide frequency range operation of the microfluidic device. The 3D pDEP chips were fabricated by making 3D structures in silicon using reactive ion etching. The reusable electrodes are deposited on second glass substrate and then aligned to the microfluidic channel to capacitively couple the electric signal through a 100 lm glass slide. The 3D insulating structures generate high electric field gradients, which ultimately increases the DEP force. To demonstrate the capabilities of 3D pDEP, Staphylococcus aureus was trapped from water samples under varied electrical environments. Trapping efficiencies of 100% were obtained at flow rates as high as 350 ll/h and 70% at flow rates as high as 750 ll/h. Additionally, for live bacteria samples, 100% trapping was demonstrated over a wide frequency range from 50 to 400 kHz with an amplitude applied signal of 200 Vpp. 20% trapping of bacteria was observed at applied voltages as low as 50 Vpp. We demonstrate selective trapping of live and dead bacteria at frequencies ranging from 30 to 60 kHz at 400 Vpp with over 90% of the live bacteria trapped while most of the dead bacteria escape. V C 2015 AIP Publishing LLC.

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Section: Introductionmentioning

confidence: 99%

Three dimensional passivated-electrode insulator-based dielectrophoresis

et al. 2015

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“…These arrays use dielectrophoretic forces to specified bioparticles (e.g., cells) and hold them in well-defined microregions. Such a platform has wide applications in cell engineering (e.g., genome editing), evaluation of chemotherapeutic reagents for cancer therapy, analysis of apoptosis, drug testing, and other phenotypic assays at the single-cell level (53). The designed multiplex single-bioparticle trapping chambers consist of an array of facing electrodes configuration (Fig.…”

Section: Resultsmentioning

confidence: 99%

Multifunctional, inexpensive, and reusable nanoparticle-printed biochip for cell manipulation and diagnosis

Esfandyarpour

DiDonato

Yang

et al. 2017

Proc. Natl. Acad. Sci. U.S.A.

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Isolation and characterization of rare cells and molecules from a heterogeneous population is of critical importance in diagnosis of common lethal diseases such as malaria, tuberculosis, HIV, and cancer. For the developing world, point-of-care (POC) diagnostics design must account for limited funds, modest public health infrastructure, and low power availability. To address these challenges, here we integrate microfluidics, electronics, and inkjet printing to build an ultra-low-cost, rapid, and miniaturized lab-ona-chip (LOC) platform. This platform can perform label-free and rapid single-cell capture, efficient cellular manipulation, rare-cell isolation, selective analytical separation of biological species, sorting, concentration, positioning, enumeration, and characterization. The miniaturized format allows for small sample and reagent volumes. By keeping the electronics separate from microfluidic chips, the former can be reused and device lifetime is extended. Perhaps most notably, the device manufacturing is significantly less expensive, timeconsuming, and complex than traditional LOC platforms, requiring only an inkjet printer rather than skilled personnel and clean-room facilities. Production only takes 20 min (vs. up to weeks) and $0.01-an unprecedented cost in clinical diagnostics. The platform works based on intrinsic physical characteristics of biomolecules (e.g., size and polarizability). We demonstrate biomedical applications and verify cell viability in our platform, whose multiplexing and integration of numerous steps and external analyses enhance its application in the clinic, including by nonspecialists. Through its massive cost reduction and usability we anticipate that our platform will enable greater access to diagnostic facilities in developed countries as well as POC diagnostics in resource-poor and developing countries.lab on a chip | point of care | diagnostics | nanoparticles | microfluidics

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“…[67][68][69] DEP separation and enrichment of CTCs features high specificity and viability. DEP is the electrokinetic motion that occurs when a polarizable particle is placed in the non-uniform electric fields.…”

Section: Dielectrophoresismentioning

confidence: 99%

Microfluidics cell sample preparation for analysis: Advances in efficient cell enrichment and precise single cell capture

et al. 2017

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Single cell analysis has received increasing attention recently in both academia and clinics, and there is an urgent need for effective upstream cell sample preparation. Two extremely challenging tasks in cell sample preparation-highefficiency cell enrichment and precise single cell capture-have now entered into an era full of exciting technological advances, which are mostly enabled by microfluidics. In this review, we summarize the category of technologies that provide new solutions and creative insights into the two tasks of cell manipulation, with a focus on the latest development in the recent five years by highlighting the representative works. By doing so, we aim both to outline the framework and to showcase example applications of each task. In most cases for cell enrichment, we take circulating tumor cells (CTCs) as the target cells because of their research and clinical importance in cancer. For single cell capture, we review related technologies for many kinds of target cells because the technologies are supposed to be more universal to all cells rather than CTCs. Most of the mentioned technologies can be used for both cell enrichment and precise single cell capture. Each technology has its own advantages and specific challenges, which provide opportunities for researchers in their own area. Overall, these technologies have shown great promise and now evolve into real clinical applications. Published by AIP Publishing. [http://dx

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Separation of tumor cells with dielectrophoresis-based microfluidic chip

Cited by 164 publications

References 43 publications

Three dimensional passivated-electrode insulator-based dielectrophoresis

Three dimensional passivated-electrode insulator-based dielectrophoresis

Multifunctional, inexpensive, and reusable nanoparticle-printed biochip for cell manipulation and diagnosis

Microfluidics cell sample preparation for analysis: Advances in efficient cell enrichment and precise single cell capture

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