Tumor necrosis factor‐α and interleukin‐1β increases CTRP1 expression in adipose tissue

Abstract

CTRP1, a member of the CTRP superfamily, consists of an N-terminal signal peptide sequence followed by a variable region, a collagen repeat domain, and a C-terminal globular domain. CTRP1 is expressed at high levels in adipose tissues of LPS-stimulated Sprague-Dawley rats. The LPSinduced increase in CTRP1 gene expression was found to be mediated by TNF-a and IL-1b. Also, a high level of expression of CTRP1 mRNA was observed in adipose tissues of Zucker diabetic fatty (fa/fa) rats, compared to Sprague-Dawley rats in the absence of LPS stimulation. These findings indicate that CTRP1 expression may be associated with a low-grade chronic inflammation status in adipose tissues.

Elevated circulating levels of CTRP1, a novel adipokine, in diabetic patients

“…In addition, CTRP1 has exhibited significant association with BMI, adiponectin and TNF-α in diabetic animal models [4,7]. However, there are no published studies addressing CTRP1 levels in type 2 diabetic patients.…”

Adipose tissue-derived cytokines, CTRPs as biomarkers and therapeutic targets in metabolism and the cardiovascular system

“…In contrast to suppression of CTRP6 expression by cytokines, cytokines IL-1β and TNF-α elevate the expression of CTRP1 adaptively in the adipose tissue by which CTRP1 suppresses inflammation [30] . Meanwhile, CTRP1 impedes collagen-induced platelet coagulation, indicating its potent therapeutic value for treating vascular disorders during the inflammatory process [31] .…”

Podocyte-specific Expression of C1q/TNF-α Related Protein 1 in Mice

“…Rabbit anti-CTRP1 polyclonal antibody was prepared for immunohistochemistry [13,14]. Briefly, mouse CTRP1 cDNA encoding the collagen repeat domain was introduced into the pET-28a bacterial expression vector (Novagen, Darmstadt, Germany), which was then transformed into E. coli strain BL21(λDE3).…”