Podocyte-specific Expression of C1q/TNF-α Related Protein 1 in Mice

Cho, Keun-Ja; Yang, Young; Lee, Young Ho; Kim, Soo-Il

doi:10.11637/kjpa.2013.26.4.147

Cited by 2 publications

(3 citation statements)

References 22 publications

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“…In more recent single‐cell transcriptomics of healthy mouse kidney, the podocyte is also a major cell type within the kidney that expresses Ctrp1 / C1qtnf1 (GEO database #http://www.ncbi.nlm.nih.gov/geo/query/acc.cgi?acc=GSE107585; data can be accessed at: 18.217.22.69/sc). Consistent with transcriptomics data, previous immunohistochemical analysis using an anti‐CTRP1 antibody also suggested podocyte as a possible cell type within the kidney that expresses CTRP1 . Thus, based on mRNA and protein data, podocytes are the principal cell type expressing CTRP1 in the glomerulus.…”

Section: Resultssupporting

confidence: 75%

“…In the kidney, only glomeruli appear to express Ctrp1 transcripts. Although there are multiple different cell types that comprise the glomerulus, single‐cell RNA‐seq as well as immunohistochemical localization of CTRP1 indicate podocytes as the major cell source of CTRP1 transcript and protein. Local production of CTRP1 raises the question of whether its expression in the kidney is regulated in pathophysiological states.…”

Section: Discussionmentioning

confidence: 99%

“…Consistent with transcriptomics data, previous immunohistochemical analysis using an anti-CTRP1 antibody also suggested podocyte as a possible cell type within the kidney that expresses CTRP1. 74 Thus, based on mRNA and protein data, podocytes are the principal cell type expressing CTRP1 in the glomerulus.…”

Section: Ctrp1 Is Expressed In Glomeruli and Podocytesmentioning

confidence: 99%

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Late‐onset renal hypertrophy and dysfunction in mice lacking CTRP1

et al. 2019

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diameter at end of diastole; LVESD, LV chamber diameter at end of systole; PWTED, posterior wall thickness at end of diastole; PWV, pulse wave velocity; RWT, relative wall thickness; SV, stroke volume; WT, wild-type. AbstractLocal and systemic factors that influence renal structure and function in aging are not well understood. The secretory protein C1q/TNF-related protein 1 (CTRP1) regulates systemic metabolism and cardiovascular function. We provide evidence here that CTRP1 also modulates renal physiology in an age-and sex-dependent manner. In mice lacking CTRP1, we observed significantly increased kidney weight and glomerular hypertrophy in aged male but not female or young mice. Although glomerular filtration rate, plasma renin and aldosterone levels, and renal response to water restriction did not differ between genotypes, CTRP1-deficient male mice 2658 | RODRIGUEZ Et al.

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Section: Resultssupporting

confidence: 75%

Section: Discussionmentioning

confidence: 99%

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Late‐onset renal hypertrophy and dysfunction in mice lacking CTRP1

et al. 2019

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Aging and chronic high-fat feeding negatively affect kidney size, function, and gene expression in CTRP1-deficient mice

Rodriguez

Little

Daneshpajouhnejad

et al. 2021

American Journal of Physiology-Regulatory, Integrative and Comp

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C1q/TNF-related protein 1 (CTRP1) is an endocrine factor with metabolic, cardiovascular, and renal functions. We previously showed that aged Ctrp1 knockout (KO) mice fed a control low-fat diet develop renal hypertrophy and dysfunction. Since aging and obesity adversely affects various organ systems, we hypothesized that aging, in combination with obesity induced by chronic high-fat feeding, would further exacerbates renal dysfunction in CTRP1-deficient animals. To test this, we fed wild-type and Ctrp1 KO mice a high-fat diet for eight months or longer. Contrary to our expectation, no differences were observed in blood pressure, heart function, or vascular stiffness between genotypes. Loss of CTRP1, however, resulted in ~2-fold renal enlargement (relative to body weight), ~60% increase in urinary total protein content and elevated pH, and changes in renal gene expression affecting metabolism, signaling, transcription, cell adhesion, solute and metabolite transport, and inflammation. Assessment of glomerular integrity, the extent of podocyte foot process effacement, as well as renal response to water restriction and salt loading did not reveal significant differences between genotypes. Interestingly, blood platelet, white blood cell, neutrophil, lymphocyte, and eosinophil counts were significantly elevated, whereas mean corpuscular volume and hemoglobin were reduced in Ctrp1-KO mice. Cytokine profiling revealed increased circulating levels of CCL17 and TIMP-1 in KO mice. Compared to our previous study, current data suggest that chronic high-fat feeding affects renal phenotypes differently than similarly aged mice fed a control low-fat diet, highlighting a diet-dependent contribution of CTRP1 deficiency to age-related changes in renal structure and function.

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Podocyte-specific Expression of C1q/TNF-α Related Protein 1 in Mice

Cited by 2 publications

References 22 publications

Late‐onset renal hypertrophy and dysfunction in mice lacking CTRP1

Late‐onset renal hypertrophy and dysfunction in mice lacking CTRP1

Aging and chronic high-fat feeding negatively affect kidney size, function, and gene expression in CTRP1-deficient mice

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