Tumor Diagnosis Using Electrophoretic Mobility Test (EMT)

Ax, W.

doi:10.1007/978-3-642-67166-1_20

Cited by 3 publications

(2 citation statements)

References 31 publications

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“…Therefore, in any testing of supernates for their ability to modify the electrophoretic mobility of indicator particles, the incubation period of the lymphocytes appears critical and must be standardized. This is particularly relevant since MBP itself has strong effects on electrophoretic mobility [1,7,8].…”

Section: Discussionmentioning

confidence: 99%

The electrophoretic mobility test with myelin basic protein, binding of125I-MBP to lymphocytes, and gel electrophoresis pattern of supernatant during incubation

Mross

Wolfrum³

et al. 1980

Res. Exp. Med.

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Lymphocytes from seven patients with malignancies, from seven patients with non-malignancies, and from five healthy persons were incubated with 125I-labeled MBP. Binding of MBP of lymphocytes was monitored from 0.5 to 20h. The binding ranged from 4 to 7% of the total MBP present and remained fairly constant during the first 4h of incubation. It dropped considerably at 20h. Mean percentages of MBP binding were lower in cancer patients than in persons without malignancies. After incubation, the supernates were examined by gel electrophoresis. The electrophoretic pattern was similar in comparing groups with different diagnoses. A considerable loss of MBP in the terminal supernatant (20h) was found. When tested in the electrophoretic mobility test (EM test) with stabilized erythrocytes, supernatant derived from cancer patients produced a somewhat higher mean slowing effect than did superntes from other patients and controls.

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Section: Discussionmentioning

confidence: 99%

The electrophoretic mobility test with myelin basic protein, binding of125I-MBP to lymphocytes, and gel electrophoresis pattern of supernatant during incubation

Mross

Wolfrum³

et al. 1980

Res. Exp. Med.

View full text Add to dashboard Cite

show abstract

“…On the other hand no test can be specific even for one kind of cancer, as the 'cancer antigen', the tumourspecific antigen is not yet found. Many groups nevertheless found quite encouraging results with the EM test in cancer diagnosis, staging and therapy control [Prichard et al, 1972;Lampert et al, 1977], But the small number of cases (because of the time-con suming techniques) or different antigens and cell electrophoresis techniques made compar ison difficult [Ax, 1979].…”

Section: Discussionmentioning

confidence: 99%