Improvement of the Electrophoretic Mobility Test to Measure Lymphocyte Sensitization

Zwergel, Th.; Zwergel, U.; Bichler, K.-H.

doi:10.1159/000280764

Cited by 1 publication

(1 citation statement)

References 8 publications

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“…The EM test was performed according to Zwergel et al [14], using ETS as indicator cells. Incubation samples and PBS controls were measured alternatively.…”

Section: Em Testmentioning

confidence: 99%

The electrophoretic mobility test with myelin basic protein, binding of125I-MBP to lymphocytes, and gel electrophoresis pattern of supernatant during incubation

Mross

Wolfrum³

et al. 1980

Res. Exp. Med.

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Lymphocytes from seven patients with malignancies, from seven patients with non-malignancies, and from five healthy persons were incubated with 125I-labeled MBP. Binding of MBP of lymphocytes was monitored from 0.5 to 20h. The binding ranged from 4 to 7% of the total MBP present and remained fairly constant during the first 4h of incubation. It dropped considerably at 20h. Mean percentages of MBP binding were lower in cancer patients than in persons without malignancies. After incubation, the supernates were examined by gel electrophoresis. The electrophoretic pattern was similar in comparing groups with different diagnoses. A considerable loss of MBP in the terminal supernatant (20h) was found. When tested in the electrophoretic mobility test (EM test) with stabilized erythrocytes, supernatant derived from cancer patients produced a somewhat higher mean slowing effect than did superntes from other patients and controls.

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“…The EM test was performed according to Zwergel et al [14], using ETS as indicator cells. Incubation samples and PBS controls were measured alternatively.…”

Section: Em Testmentioning

confidence: 99%

The electrophoretic mobility test with myelin basic protein, binding of125I-MBP to lymphocytes, and gel electrophoresis pattern of supernatant during incubation

Mross

Wolfrum³

et al. 1980

Res. Exp. Med.

View full text Add to dashboard Cite

show abstract

Improvement of the Electrophoretic Mobility Test to Measure Lymphocyte Sensitization

Cited by 1 publication

References 8 publications

The electrophoretic mobility test with myelin basic protein, binding of125I-MBP to lymphocytes, and gel electrophoresis pattern of supernatant during incubation

The electrophoretic mobility test with myelin basic protein, binding of125I-MBP to lymphocytes, and gel electrophoresis pattern of supernatant during incubation

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