Tulsi (Ocimum sanctum) Leaf Ethanol Extract Reduces Inflammatory Cell Infiltration in Aspirin-Induced Gastritis Rats

Abstract

<p class="ISIABSTRAKINGGRIS">Gastritis is an inflammation of the gastric mucosa. Tulsi leaf extract has phenol, flavonoid and saponin compounds which are potential as antioxidant and increase defensive factors in the gastric. The purpose of this research was to find out the effect of tulsi (Ocimum sanctum) leaf extract in polymorphonuclear (PMN) inflammatory cell infiltration in gastric of aspirin-induced gastritis rat model. This study was laboratory experimental research using post-test only control group design. Randomly, 27 male rats were divided into 3 groups, the first group was not induced by aspirin and extract as negative control, the second group was induced by aspirin of 600 mg/kgBW as positive control, and the third group was induced by aspirin of 600 mg/kgBW and was given Ocimum sanctum extract at a dose of 400 mg/kgBW as treatment group. Gastric of the rats were taken on 16th day for histopathology evaluation using hematoxylin and eosin (HE) staining. Evaluation was done by calculating the PMN inflammatory cell infiltration in mucosal and submucosal layer. The results of the average number of PMN inflammatory cell in the gastric tissue of the treatment group showed a significant decrease compared to the positive and negative control groups with P-value <0.05. This study proved that Ocimum sanctum leaf extract administration with the dose of 400 mg/kgBW can decrease gastritis inflammation by reducing PMN inflammatory cell in gastric of aspirin-induced gastritis rat model.</p>

Administration of Holy Basil (Ocimum Sanctum L.) Leaves Extract Inhibiting the Enhancement of Malondialdehyde Level and Steatosis Number in Male Wistar Rats Given Used Cooking Oil

“…8 Another research by Festi et al showed that administration of Ocimum sanctum leaf extract at a dose of 400 mg/kg BW can reduce the degree of inflammation by decreasing the average number of infiltrating PMD inflammatory cells in the stomach of gastritis rat models. 16 This strengthens the theory that a dose of 400 mg/kg BW is an effective dose in inhibiting steatosis because it also inhibits inflammatory pathways.…”

Antihyperglycemic, Endothelial protection and Toxicity study of Basil Leaves Extract on Diabetic Rats

“…Basil leaves, herbs from the Ocimum family have been proposed to possess antihyperglycemic properties in multiple studies [16], [17], [20], [29], [30], [31]. This study found significant blood glucose -lowering effect and endothelial protection of basil leaves.…”

“…Insulin-secretory effect of the Ocimum family was elucidated by Hannan et al, as the extract enhances effects of insulin secretion from the pancreas, isolated islets, and modulation of intracellular calcium [30]. Basil leaves also shown to possess insulinotropic effect through the enhancement of physiological pathways on pancreatic beta-cells [20]. Thus, increasing plasma insulin and decreasing BGL in rats with type 2 diabetes [30].…”

“…Indonesia's archipelago and plethora of diverse medicinal plants have pioneered various studies on traditional medicines, including the usage of readily available basil leaves [20], [21], [22], [23]. Hence, it is imperative to further investigate basil leaves efficacy in the study using rats models.…”

Effect of Basil Leaf Extract on Diabetes: A Systematic Review and Meta-Analysis

“…20,21 The extract also enhances physiological pathways on pancreatic β-cells, indicating its insulinotropic potency. 22 Ezeani et al showed that basil leaf extract could inhibit α-amylase and α-glucosidase, which suppress carbohydrate metabolism and the consequent glucose release from the small intestine lumen, leading to its postprandial anti-hyperglycaemic effect. 12 Basil leaf extract exhibited safety and similar efficacy when administered both acutely and chronically.…”