Trypsin Hydrolysed Protein Fractions as Radical Scavengers and Anti-bacterial Agents from Ficus deltoidea

Abdullah, Farah Izana; Chua, Lee Suan; Rahmat, Zaidah; Soontorngun, Nitnipa; Somboon, Pichayada

doi:10.1007/s10989-017-9613-5

Cited by 4 publications

(5 citation statements)

References 49 publications

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“…There is a lack of reports in the literature concerning the antioxidant capacity of trypsin‐hydrolyzed CS proteins. However, our observation of the enhancement of radical scavenging activities following trypsin hydrolysis is consistent with those reported in medicinal herb Ficus deltoidea [13], lupin [36], flaxseed [37] and porcine liver [38]. Enhanced ferric reducing activity following tryptic hydrolysis was found for flaxseed [37] and porcine liver [38] proteins, which is a contrast to our observation on CS proteins.…”

Section: Resultssupporting

confidence: 92%

“…To fill the aforementioned gap of knowledge on the antioxidant potential of CS protein hydrolysates, this study was conducted to examine the antioxidant activities of Trypsin-hydrolyzed CS proteins by using a combination of in vitro chemical-and human red blood cell-based assays. Trypsin was used for proteolysis as it has been commonly and successfully used to produce food and nonfood protein hydrolysates exhibiting antioxidant activities [10][11][12][13][14]. The use of multiple assays of different principles may reveal different modes of antioxidant action exerted by the CS protein hydrolysate.…”

Section: Introductionmentioning

confidence: 99%

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Trypsin‐hydrolyzed Corn Silk Proteins: Antioxidant Activities, in vitro Gastrointestinal and Thermal Stability, and Hematoprotective Effects

Chai

Ang

Goh

et al. 2020

eFood

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Hematoprotection lipid peroxidation protein hydrolysate simulated gastrointestinal digestion stigma maydis thermal stability A B S T R A C TThe aim of this study was to examine the antioxidant capacity of Trypsin-hydrolyzed corn silk proteins, specifically radical scavenging, ferric reducing and anti-lipid peroxidation activities, as well as stability after heating and simulated gastrointestinal digestion. Among the 1-5-h hydrolysates, the 1-h Trypsin hydrolysate (T1H) was the strongest. T1H exhibited stronger H 2 O 2 [half maximal effective concentration (EC 50 ) = 156.44 µg/mL] and superoxide (EC 50 = 0.33 mg/mL) scavenging activities than glutathione, carnosine and N-acetylcysteine. Radical scavenging activities of T1H were heat-stable (25-100°C), although lost by 20-58% after gastrointestinal digestion. Ferric reducing activity of T1H was heat-stable, even enhanced by 20% after gastrointestinal digestion. Lecithin liposome assay found anti-lipid peroxidation activity of T1H resistant to gastrointestinal peptidases. By contrast, 100°C incubation reduced anti-lipid peroxidation activity of 0.5 mg/mL T1H to 3.5-fold lower than that of 25°C incubation. At 30 µg/mL, T1H (3% hemolysis) was stronger than glutathione (32% hemolysis) in protecting human red blood cells. T1H was 1.5-fold more potent than glutathione and carnosine in alleviating lipid peroxidation in the cells. Overall, T1H demonstrated strong, heat-stable radical scavenging activities, besides partial resistance to gastrointestinal peptidases. T1H was also hematoprotective. T1H is a promising antioxidant for the development of functional food ingredients and nutraceuticals.

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Section: Resultssupporting

confidence: 92%

Section: Introductionmentioning

confidence: 99%

Trypsin‐hydrolyzed Corn Silk Proteins: Antioxidant Activities, in vitro Gastrointestinal and Thermal Stability, and Hematoprotective Effects

Chai

Ang

Goh

et al. 2020

eFood

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“…Although total surface area is a very important factor that can affect the amount of protein adsorbed on the scaffold, we believe that the pore size and water flow rate are also very important factors to consider. For example, the average size of one of the most abundant proteins in the medium, bovine serum albumin (BSA), is 66.5 kDa (~ 7.1 nm) [ 79 ]. Although the size of BSA is smaller than the pore size of SNP100 (~ 16.5 nm), the effective size of BSA may be larger due to intermolecular interactions between BSA molecules and hydration in the solution [ 80 , 81 ].…”

Section: Resultsmentioning

confidence: 99%

Natural bone-mimicking nanopore-incorporated hydroxyapatite scaffolds for enhanced bone tissue regeneration

et al. 2022

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Background A considerable number of studies has been carried out to develop alloplastic bone graft materials such as hydroxyapatite (HAP) that mimic the hierarchical structure of natural bones with multiple levels of pores: macro-, micro-, and nanopores. Although nanopores are known to play many essential roles in natural bones, only a few studies have focused on HAPs containing them; none of those studies investigated the functions of nanopores in biological systems. Method We developed a simple yet powerful method to introduce nanopores into alloplastic HAP bone graft materials in large quantities by simply pressing HAP nanoparticles and sintering them at a low temperature. Results The size of nanopores in HAP scaffolds can be controlled between 16.5 and 30.2 nm by changing the sintering temperature. When nanopores with a size of ~ 30.2 nm, similar to that of nanopores in natural bones, are introduced into HAP scaffolds, the mechanical strength and cell proliferation and differentiation rates are significantly increased. The developed HAP scaffolds containing nanopores (SNPs) are biocompatible, with negligible erythema and inflammatory reactions. In addition, they enhance the bone regeneration when are implanted into a rabbit model. Furthermore, the bone regeneration efficiency of the HAP-based SNP is better than that of a commercially available bone graft material. Conclusion Nanopores of HAP scaffolds are very important for improving the bone regeneration efficiency and may be one of the key factors to consider in designing highly efficient next-generation alloplastic bone graft materials.

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“…The removal of ROS by the hydrolysed protein fractions was superior to that by the unhydrolyzed protein fractions [19]. Based on a one-way analysis of variance, only the protein hydrolysates of 30 and 100 kDa indicated significant differences in radical scavenging capacities [19]. Methanolic leaf extract had the highest antioxidants for ferric reducing antioxidant power (FRAP) (6-9 mmol Fe 2+ /g), 2,2'-azino-bis (3-ethylbenzothiazoline-6-sulfonic acid) (ABTS) (2-3 mmol TE/g), and 2,2-diphenyl-1-picrylhydrazyl (DPPH) (EC50:200-410 μg/mL) [12].…”

Section: Antioxidants Activitymentioning

confidence: 91%

“…Antioxidant activities of F. deltoidea have been documented in several investigations, but the portion of the plant utilized in the vast majority of the studies was the leaf [18]. The removal of ROS by the hydrolysed protein fractions was superior to that by the unhydrolyzed protein fractions [19]. Based on a one-way analysis of variance, only the protein hydrolysates of 30 and 100 kDa indicated significant differences in radical scavenging capacities [19].…”

Section: Antioxidants Activitymentioning

confidence: 99%

Crude extract of Ficus deltoidea Jack (FD) as a natural biological therapy

Abdulrahman

2023

Exploration of Targeted Anti-Tumor Therapy

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Aim: This study shows how important it is to coordinate research on Ficus deltoidea Jack (FD) so that results from different sources can be compared directly and a scientific conclusion can be made. Methods: The author looked for research papers on Ficus (F.) deltoidea on Google Scholar, Science Direct, Google.com, Wiley, PubMed, Hindawi, Springer, and other related databases. This analysis excludes data that cannot be trusted, thesis papers, and review articles about F. deltoidea. Results: In traditional medicine, the plant’s leaves and syconia are used to cure a wide variety of ailments, including itchiness, diarrhoea, cancer, sexual dysfunction, age-related issues, malaria, cancer, anxiety, pain, constipation, fever, diabetes, tooth pain, and tooth decay. In vitro and in vivo studies showed the effectiveness of the leaves against cancer cell lines. Conclusions: Based on the existing research on the health benefits of FD, it is critical to focus on its more active constituents and their identification, determination, further development, and, most importantly, standardization of the leaves for the management and treatment of cancer and its related cases. More research is needed before it can be considered a promising herbal source of novel medication candidates for treating various disorders.

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Trypsin Hydrolysed Protein Fractions as Radical Scavengers and Anti-bacterial Agents from Ficus deltoidea

Cited by 4 publications

References 49 publications

Trypsin‐hydrolyzed Corn Silk Proteins: Antioxidant Activities, in vitro Gastrointestinal and Thermal Stability, and Hematoprotective Effects

Trypsin‐hydrolyzed Corn Silk Proteins: Antioxidant Activities, in vitro Gastrointestinal and Thermal Stability, and Hematoprotective Effects

Natural bone-mimicking nanopore-incorporated hydroxyapatite scaffolds for enhanced bone tissue regeneration

Crude extract of Ficus deltoidea Jack (FD) as a natural biological therapy

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