Hematoprotection lipid peroxidation protein hydrolysate simulated gastrointestinal digestion stigma maydis thermal stability A B S T R A C TThe aim of this study was to examine the antioxidant capacity of Trypsin-hydrolyzed corn silk proteins, specifically radical scavenging, ferric reducing and anti-lipid peroxidation activities, as well as stability after heating and simulated gastrointestinal digestion. Among the 1-5-h hydrolysates, the 1-h Trypsin hydrolysate (T1H) was the strongest. T1H exhibited stronger H 2 O 2 [half maximal effective concentration (EC 50 ) = 156.44 µg/mL] and superoxide (EC 50 = 0.33 mg/mL) scavenging activities than glutathione, carnosine and N-acetylcysteine. Radical scavenging activities of T1H were heat-stable (25-100°C), although lost by 20-58% after gastrointestinal digestion. Ferric reducing activity of T1H was heat-stable, even enhanced by 20% after gastrointestinal digestion. Lecithin liposome assay found anti-lipid peroxidation activity of T1H resistant to gastrointestinal peptidases. By contrast, 100°C incubation reduced anti-lipid peroxidation activity of 0.5 mg/mL T1H to 3.5-fold lower than that of 25°C incubation. At 30 µg/mL, T1H (3% hemolysis) was stronger than glutathione (32% hemolysis) in protecting human red blood cells. T1H was 1.5-fold more potent than glutathione and carnosine in alleviating lipid peroxidation in the cells. Overall, T1H demonstrated strong, heat-stable radical scavenging activities, besides partial resistance to gastrointestinal peptidases. T1H was also hematoprotective. T1H is a promising antioxidant for the development of functional food ingredients and nutraceuticals.
The high dropout rate among undergraduates has drawn the concerns of policymakers and educators. Studies suggested that the dropout is relevant to poor academic adjustment, and to poor academic adjustment is associated with perfectionism and coping strategies. This study adopts the personality-coping-outcome theory as a framework to examine whether coping strategies mediate the effects of perfectionism on academic adjustment. Two hundred eleven undergraduate students participated in the online survey through the purposive sampling method. They were asked to fill in the Short-Almost perfect scale, simplified coping style questionnaire and academic adjustment scale. The results showed that both perfectionism and coping strategies are associated with academic adjustment, and perfectionism is associated with coping strategies. Besides, coping strategies are statistical mediators for the effects of perfectionism on academic adjustment. The findings support the application of personality-coping-outcome theory in the areas of academic adjustment. Workshops can be provided to students to train their use of appropriate coping strategies while facing academic adjustment issues.
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