Trypanosoma evansi experimental infection in the South American coati (Nasua nasua): clinical, parasitological and humoral immune response

Herrera, Heitor Miraglia; Aquino, Lúcia Padilha Cury Thomaz de; Menezes, Renato; Marques, Luiz Carlos; Moraes, Marcela Barbosa de; Werther, Karin; Machado, R. Z.

doi:10.1016/s0304-4017(01)00532-5

Cited by 26 publications

(16 citation statements)

References 15 publications

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“…Vários trabalhos implicam as capivaras como importantes reservatórios para T. evansi (Hoare 1972, Morales et al 1976, Nunes et al 1993, Franke et al 1994, Muñoz & Chavez 2001. Capivaras, e também quatis, geralmente desenvolvem uma forma crônica da doença e podem apresentar infecções subclínicas (Nunes et al 1993, Franke et al 1994, Herrera et al 2001, Muñoz & Chávez 2001. Segundo relatos de pecuaristas pantaneiros, surtos de tripanossomíase em eqüinos geralmente são precedidos pela doença em capivaras (Silva et al 2002).…”

Section: Discussão Discussão Discussão Discussão Discussãounclassified

“…Outros animais poderiam participar na transmissão de T. evansi para os eqüinos, como os bovinos e os cães, que podem ser reservatórios eficientes pelo contato estreito com os eqüinos (Franke et al 1994); os quatis, que podem também abrigar o parasita (Nunes et al 1993, Herrera et al 2001; e, na América do Sul, os morcegos hematófagos, que podem atuar tanto como reservatórios quanto como vetores para T. evansi (Hoare 1972). É, no entanto, pouco provável que esses reservatórios estivessem envolvidos ou que tivessem papel importante na epidemiologia dos surtos aqui descritos.…”

Section: Discussão Discussão Discussão Discussão Discussãounclassified

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Surtos de tripanossomíase por Trypanosoma evansi em eqüinos no Rio Grande do Sul: aspectos epidemiológicos, clínicos, hematológicos e patológicos

et al. 2005

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Additionally, around 80 mares were sent to Farm A to be bred. Of those, 66 became ill and 56 died after being returned to their farms of origin. Twenty one horses clinically affected by the disease were observed. Clinical signs included loss of weight (despite voracious appetite), lethargy, incoordination and instability of hindlimbs, atrophy of the large muscles of the hindlimbs, muscle weakness and paleness of mucosae. Specimens of T. evansi were detected in the blood drawn from four affected horses. Normocytic normochromic anemia with PCVs ranging from 15 to 31%, leucocytosis due to lymphocytosis associated to large atypical lymphocytes was observed in several affected horses. High levels of antibodies against T. evansi were detected in the serum of six horses from Farm A. Eight horses presented encephalic neurological signs such as circling, ataxia, blindness, excitation, falls, listlessness, proprioception deficits and head tilt. One horse assumed a "dogseating position". Necropsy findings included muscle atrophy, enlargement and lymphoid hyperplasia of the spleen and lymphnodes, edema and softening of the white and grey matter of the brain. Histologically, an overwhelming necrotizing panencephalitis was observed in the seven horses with encephalic signs. This panencephalitis was characterized by marked edema, demyelination and necrosis and perivascular infiltrates of 6-10 layers of lymphocytes and plasm cells affecting both the white and gray matter. Several plasm cells in the inflammatory infiltrate contained numerous eosinophilic globules in their cytoplasm (Mott cells). Similar histological lesions were observed in the spinal cord of the horse with the "dog-seating position". The brains of five horses with the encephalic signs were submitted to immunohistochemistry stain by the streptavidinbiotin technique. In all of those five brains moderate to abundant specimens of T. evansi in the perivascular spaces and neuropile were marked by the specific antibody. Epidemiological, clinical, hematological, and pathological aspects of equine trypanosomiasis caused by T. evansi are discussed.

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Surtos de tripanossomíase por Trypanosoma evansi em eqüinos no Rio Grande do Sul: aspectos epidemiológicos, clínicos, hematológicos e patológicos

et al. 2005

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“…Serum was stored at -20 °C. Serum samples obtained in previous studies of experimental T. evansi infection in dogs (AQUINO et al, 1999), bovines (POCHINI, 2000), coatis (HERRERA et al, 2001), and donkeys (CADIOLI, 2001) were used. Sera obtained from the experimentally infected animals before inoculation and on different days during infection were used in Western blotting and as reference negative and positive control sera in serology.…”

Section: Animalsmentioning

confidence: 99%

Antigenic characterization of Trypanosoma evansi using sera from experimentally and naturally infected bovines, equines, dogs, and coatis

Aquino¹,

Machado²,

Lemos³

et al. 2010

Rev. Bras. Parasitol. Vet. (Online)

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The present research investigated the presence of T. evansi antibodies in animals from the subregion of Nhecolandia, in the Pantanal Sul-mato-grossense, by means of an enzyme linked immunosorbent assay (ELISA) and indirect immunofluorescence antibody test (IFAT), and the pattern of polypeptide recognition by sera from experimentally and naturally infected hosts using Western blotting. Serum samples were obtained from bovines (n = 102), horses (n = 98), and dogs (n = 55), and from 32 free-ranging coatis (Nasua nasua). None of the bovines were found positive, while sera from 16 dogs (29%) and 23 horses (23.4%) were positive by ELISA. Sera from 8 coatis (25%) were found positive using IFAT. Western blotting revealed major polypeptides of T. evansi with molecular weight ranging from 74 to 38 kDa. The polypeptides of 66, 48-46, and 38 kDa were identified by sera from experimentally infected bovines, donkeys, dogs, and coatis. The 48-46 and 38 kDa bands were mainly recognized in chronic phase of infection. The antigen with apparent molecular weight of 66 kDa, revealed by antibodies from all experimental animals, was also recognized in sera of horses and dogs from the Pantanal. The 48-46 kDa polypeptide was identified by antibodies from all naturally infected animals and must be further evaluated for use in specific diagnosis of T. evansi infection.Keywords: Trypanosoma evansi, ELISA-test, IFAT, Pantanal mato-grossense, antigenic characterization. ResumoO trabalho de pesquisa investigou a presença de anticorpos anti-T. evansi em animais da sub-região da Nhecolândia, no Pantanal sul-mato-grossense, pelo ensaio imunoenzimático (ELISA) e a reação de imunofluorescência indireta (RIFI). O reconhecimento de polipeptídeos de T. evansi foi realizado pela técnica de "Western blotting", utilizando soros de animais experimentalmente e naturalmente infectados. As amostras de soro foram obtidas de bovinos (n = 102), cavalos (n = 98) e cães (n = 55) e de 32 quatis de vida livre (Nasua nasua) do pantanal mato-grossense. Todos os soros dos bovinos foram negativos, enquanto soros de 16 cães (29%) e 23 cavalos (23,4%) foram positivos pelo ELISA. Soros de oito quatis (25%) foram positivos pela RIFI. Pelo "Western-blotting" foi possível revelar polipeptídeos de T. evansi, com peso molecular variando de 74 a 38 kDa. Os polipeptídeos de 66, 48-46 e 38 kDa foram identificados por soros de bovinos, cavalos, cães e quatis experimentalmente infectados. As bandas de 48-46 e 38 kDa foram reconhecidas principalmente na fase crônica da infecção. O antígeno com peso molecular aparente de 66 kDa, revelado por anticorpos de todos os animais experimentais, também foi reconhecido por soros de cavalos e cães do Pantanal. O polipeptídeo de 48-46 kDa foi identificado por anticorpos de todos os animais naturalmente infectados, devendo ser avaliado para o diagnóstico específico da infecção pelo T. evansi.

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“…Although coatis are considered reservoirs for T. evansi, previous studies have reported clinical disease in these animals, with symptoms including depression, weakness, lethargy, and pale mucous membranes (HERRERA et al, 2001). In chronic cases, experimentallyinfected coatis presented with myocarditis, encephalitis, severe anemia, and changes in serum biochemistry (HERRERA et al, 2002).…”

Section: Discussionmentioning

confidence: 99%

Molecular detection of Trypanosoma evansi (Kinetoplastida: Trypanosomatidae) in procyonids (Carnivora: Procyonidae) in Eastern Amazon, Brazil

et al. 2016

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The present study aimed to diagnose the natural infection of captive and free-living procyonids with Trypanosoma evansi in the states of Amapá and Pará, Brazil. From February 2012 to August 2013, whole blood samples and blood smears were obtained from 45 free-living procyonids and from nine procyonids kept in captivity in wild life refuges and zoobotanical parks in the states of Amapá and Pará. Whole blood samples were collected and kept at -20ºC for the detection of T. evansi DNA by PCR using the RoTat 1.2 forward and RoTat 1.2 reverse primers. In addition, the blood smears were processed and examined for the presence of trypomastigote forms of T. evansi. T. evansi DNA was detected in 18.52% (10/54) of the procyonids, namely, in captive crab-eating raccoons and captive and free-living coatis in Pará State. No trypomastigote forms were observed in the blood smears. DNA from T. evansi was detected in P. cancrivorus and N. nasua in Pará State, being this the first such report in P. cancrivorus.

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Trypanosoma evansi experimental infection in the South American coati (Nasua nasua): clinical, parasitological and humoral immune response

Cited by 26 publications

References 15 publications

Surtos de tripanossomíase por Trypanosoma evansi em eqüinos no Rio Grande do Sul: aspectos epidemiológicos, clínicos, hematológicos e patológicos

Surtos de tripanossomíase por Trypanosoma evansi em eqüinos no Rio Grande do Sul: aspectos epidemiológicos, clínicos, hematológicos e patológicos

Antigenic characterization of Trypanosoma evansi using sera from experimentally and naturally infected bovines, equines, dogs, and coatis

Molecular detection of Trypanosoma evansi (Kinetoplastida: Trypanosomatidae) in procyonids (Carnivora: Procyonidae) in Eastern Amazon, Brazil

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