Antigenic characterization of Trypanosoma evansi using sera from experimentally and naturally infected bovines, equines, dogs, and coatis

Aquino, Lúcia Padilha Cury Thomaz de; Machado, Rosangela Zacarias; Lemos, Karen Regina; Marques, Luiz Carlos; Garcia, Marcos Valério; Borges, Gustavo Puia

doi:10.1590/s1984-29612010000200009

Cited by 12 publications

(10 citation statements)

References 19 publications

(30 reference statements)

Supporting

Mentioning

Contrasting

Order By: Relevance

“…The CATT/T.evansi is a technique often used in the field. The technique is believed to be able to detect Ig M which occurs early in infection, in contrast to the ELISA technique used to detect Ig G (Aquino et al 2010). According to Desquesnes et al (2013) that these two techniques can be done together in order to obtain a powerful diagnostic conclusions.…”

Section: Serology With Catt -T Evansimentioning

confidence: 99%

Parasitic Diseases in Dairy Cattle in Cibungbulang District of West Java

Dewi

Wardhana

Sawitri

et al. 2016

Proceedings of International Seminar on Livestock Production and Veterinary Technology

View full text Add to dashboard Cite

Cibungbulang District of West Java is a region where has a population of dairy cattle and it has been pointed as one of central livestock comunity (Sentra Peternakan Rakyat, SPR). However, parasitic diseases remains a major problem at the area. The purpose of study was to determine parasitic diseases attacking Friesian Holstein (FH) dairy cattle in the Cibungbulang District of West Java. Examination of stool and blood serum should be done to strengthen the diagnosis of parasitic diseases. A total of 130 blood and faeces was collected. Stool examination was conducted using Withlock method to calculate and observe the egg shape of worms including Eimeria sp. Blood sera examination were carried out for serological tests of Trypanosoma evansi based on Card Aglutination Trypanosomiasis Test (CATT) -T. evansi method. The results showed 36.92% (48/130) FH cattle were infested by Trichostrongylus spp, 2.31% (3/130) by Toxocara vitulorum, 20% (26/130) by Eimeria sp and others were negative, 40.77% (53/130). In addition, 14.62% (19/130) sera possessed a positive response for CATT test, consisting 6.15% (8/130) of medium agglutination (+),6.92% (9/130) of strong agglutination (++) and 1.54% (2/130) of weak agglutination/dubious (±). In conclusion, FH cattle in Cibungbulang District are infested by internal parasites (worms and Eimeria sp) and are positive serologically for T. evansi. In term of management system, farmers should maintain level of environmental sanitation to increase milk production.

show abstract

Section: Serology With Catt -T Evansimentioning

confidence: 99%

Parasitic Diseases in Dairy Cattle in Cibungbulang District of West Java

Dewi

Wardhana

Sawitri

et al. 2016

Proceedings of International Seminar on Livestock Production and Veterinary Technology

View full text Add to dashboard Cite

show abstract

“…Mean corpuscular volume (MCV) was calculated based on the RBC and PCV values. The immunoglobulin concentration (IgG) was determined by titration with the indirect fluorescent antibody test (IFAT) [ 27 , 28 ] and by optical density using enzyme-linked immunosorbent assay (ELISA)[ 29 ]. Leukocyte (eosinophils, lymphocytes, monocytes, and neutrophils) counts were performed using blood smears fixed with methanol and stained with Giemsa [ 30 ].…”

Section: Methodsmentioning

confidence: 99%

“…evansi IgG antibodies in crab-eating foxes and ocelots by IFAT using a commercial fluorescein-conjugated antibody against dogs and cats IgGs, respectively. The cut-off value for IFAT was 1:40 [ 27 ]. There is presently no fluorescein-conjugated antibody against coatis’ IgGs.…”

Section: Methodsmentioning

confidence: 99%

Outcomes of Trypanosoma cruzi and Trypanosoma evansi infections on health of Southern coati (Nasua nasua), crab-eating fox (Cerdocyon thous), and ocelot (Leopardus pardalis) in the Brazilian Pantanal

et al. 2018

View full text Add to dashboard Cite

The occurrence of Trypanosoma spp. in wild carnivore populations has been intensively investigated during the last decades. However, the impact of these parasites on the health of free-living infected animals has been largely neglected. The Pantanal biome is the world’s largest seasonal wetland, harboring a great diversity of species and habitats. This includes 174 species of mammals, of which 20 belong to the order Carnivora. The present study aimed to investigate the effect of Trypanosoma evansi and Trypanosoma cruzi infections and coinfections on the health of the most abundant carnivores in the Pantanal: coati (Nasua nasua), crab-eating fox (Cerdocyon thous), and ocelot (Leopardus pardalis). We captured 39 coatis, 48 crab-eating foxes, and 19 ocelots. Diagnostic tests showed T. cruzi infection in 7 crab-eating foxes and 5 coatis. Additionally, 7 crab-eating foxes, 10 coatis, and 12 ocelots were positive for T. evansi. We observed coinfections in 9 crab-eating foxes, 8 coatis, and 2 ocelots. This is the first report of T. evansi and T. cruzi infection on the health of free-living ocelots and crab-eating foxes. We showed that single T. evansi or T. cruzi infection, as well as coinfection, caused some degree of anemia in all animals, as well as an indirect negative effect on body condition in coatis and crab-eating foxes via anemia indicators and immune investment, respectively. Furthermore, the vigorous immune investment observed in sampled coatis, crab-eating foxes and ocelots infected by T. evansi, T. cruzi and coinfected can be highly harmful to their health. Overall, our results indicate that single and combined infection with T. evansi and T. cruzi represent a severe risk to the health of wild carnivores in the Pantanal region.

show abstract

“…For the detection of anti- T. evansi IgG antibodies we used an IFAT assay on dogs and C. thous only ( Aquino et al, 2010 ). Currently there are no conjugated fluoresceine antibodies against IgG for N. nasua , S. cay , D. azarea , E. sexcinctus and D. novemcinctus .…”

Section: Methodsmentioning

confidence: 99%

Maintenance of Trypanosoma cruzi, T. evansi and Leishmania spp. by domestic dogs and wild mammals in a rural settlement in Brazil-Bolivian border

Porfírio

Santos

Macedo

et al. 2018

International Journal for Parasitology: Parasites and Wildlife

View full text Add to dashboard Cite

Domestic dogs are considered reservoirs hosts for several vector-borne parasites. This study aimed to evaluate the role of domestic dogs as hosts for Trypanosoma cruzi, Trypanosoma evansi and Leishmania spp. in single and co-infections in the Urucum settlement, near the Brazil-Bolivian border. Additionally, we evaluated the involvement of wild mammals’ in the maintenance of these parasites in the study area. Blood samples of dogs (n = 62) and six species of wild mammals (n = 36) were collected in July and August of 2015. The infections were assessed using parasitological, serological and molecular tests. Clinical examination of dogs was performed and their feeding habits were noted. Overall, 87% (54/62) of sampled dogs were positive for at least one trypanosomatid species, in single (n = 9) and co-infections (n = 45). We found that 76% of dogs were positive for T. cruzi, four of them displayed high parasitemias demonstrated by hemoculture, including one strain types TcI, two TcIII and one TcIII/TcV. Around 73% (45/62) of dogs were positive to T. evansi, three with high parasitemias as seen by positive microhematocrit centrifuge technique. Of dogs sampled, 50% (31/62) were positive for Leishmania spp. by PCR or serology. We found a positive influence of (i) T. evansi on mucous pallor, (ii) co-infection by T. cruzi and Leishmania with onychogryphosis, and (iii) all parasites to skin lesions of sampled dogs. Finally, feeding on wild mammals had a positive influence in the Leishmania spp. infection in dogs. We found that 28% (5/18) coati Nasua nasua was co-infected for all three trypanosamatids, demonstrating that it might play a key role in maintenance of these parasites. Our results showed the importance of Urucum region as a hotspot for T. cruzi, T. evansi and Leishmania spp. and demonstrated that dogs can be considered as incidental hosts.

show abstract

Antigenic characterization of Trypanosoma evansi using sera from experimentally and naturally infected bovines, equines, dogs, and coatis

Cited by 12 publications

References 19 publications

Parasitic Diseases in Dairy Cattle in Cibungbulang District of West Java

Parasitic Diseases in Dairy Cattle in Cibungbulang District of West Java

Outcomes of Trypanosoma cruzi and Trypanosoma evansi infections on health of Southern coati (Nasua nasua), crab-eating fox (Cerdocyon thous), and ocelot (Leopardus pardalis) in the Brazilian Pantanal

Maintenance of Trypanosoma cruzi, T. evansi and Leishmania spp. by domestic dogs and wild mammals in a rural settlement in Brazil-Bolivian border

Contact Info

Product

Resources

About