Trypanosoma evansi: Effect of experimental infection on the osmotic fragility, lipid peroxidation and calcium-ATPase activity of rat red blood cells

Mijares, Alfrédo; Vivas, Jeilyn; Abad, Cilia; Betancourt, Meyerling; Piñero, Sandy; Proverbio, Fulgencio; Marı́n, Reinaldo; Portillo, R.

doi:10.1016/j.exppara.2009.11.002

Cited by 23 publications

(16 citation statements)

References 22 publications

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“…43 It was presumed that the functions of NaK-ATPase and CaMg-ATPase may be affected in the infection, 1 because these membrane-associated enzymes were reduced in activities during infection in the brain, kidney and testis. [44][45][46] Mijares et al, 47 confirmed in their report that erythrocyte Ca-ATPase activity decreased in Trypanosoma evansi infection. Trypanotolerance has been associated with higher erythrocyte levels of zinc and manganese, 48 increased erythrocyte sialic acid content, 49 differential erythrocyte sialic acid composition 50 and erythrocyte surface sialic acid moieties characterised by more O-acetyl than glycolyl groups.…”

Section: 3435supporting

confidence: 65%

Evolving anti-disease strategies from biochemical pathogenesis of african trypanosomiasis

Igbokwe¹

2018

ACP

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African trypanosomes are transmitted biologically by tsetse flies (Glossina spp) to humans and animals to cause African trypanosomiasis or trypanosomosis (sleeping sickness or Ngana). The pathology associated with the disease includes blood dyscrasia (anemia, leucopenia, thrombocytopenia), organ damage and inflammation. The infected host's metabolism is altered in response to the disease. The biochemical configuration and functions of the cell membrane, mitochondria, and cytosolic components are deranged by the infection. Cells of the infected host respond biochemically by producing chemical signals which regulate biochemical pathways, influence inflammatory responses and modify structural components. Several biochemical tools have been used to elucidate the biomolecules expressed in the disease. These biomolecules are also markers for diagnostic purposes and pathophysiological evaluations. The technologies involved in the biochemical studies are expanding from basic techniques of colorimetry, spectophotometry, and chromatography to sophisticated mass spectrometry. There are new trends employing systematics such as genomics, proteomics and metabolomics (metabonomics). The goal is to identify molecules and biochemical loci which can provide avenue for anti-disease strategies needed to treat and control the disease.

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Section: 3435supporting

confidence: 65%

Evolving anti-disease strategies from biochemical pathogenesis of african trypanosomiasis

Igbokwe¹

2018

ACP

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“…The technique is used in the assessment of membrane integrity of animals having erythrocyte membrane abnormalities associated with hereditary spherocytosis, elliptocytosis and stomatocytosis [2], blood infections such as babesiosis [3], trypanosomosis [4][5][6], and anaplasmosis [7], and autoimmune haemolytic diseases [8]. Osmotic fragility of erythrocytes is also affected by xenobiotic, chemical and drug actions [9][10][11][12][13], and physiological factors such as transport stress, nutritional status, hormonal balance, species, breed and sex [14][15][16][17][18][19].…”

Section: Introductionmentioning

confidence: 99%

Influence of extracellular media’s ionic strength on the osmotic stability of Sahel goat erythrocytes

Igbokwe

2014

Journal of Basic and Clinical Physiology and Pharmacology

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“…Our study was encouraged by recent findings that correlate the progression of parasitemia with changes on parameters of oxidative stress, such as diminished levels of reduced glutathione (a well-known endogenous antioxidant) and increased lipid peroxidation in erythrocytes of rats (Anschau et al 2013) and horses infected with T. evansi (Mijares et al 2010). Knowing that resveratrol is a natural antioxidant that has been used to prevent and treat various diseases that have association with oxidative stress (Coradini et al 2014), this component was tested in our study as an attempt to reduce the toxicity of the chemotherapy commonly used for the treatment of trypanosomosis, in this case diminazene aceturate.…”

Section: Discussionmentioning

confidence: 90%

Combination of diminazene aceturate and resveratrol reduces the toxic effects of chemotherapy in treating Trypanosoma evansi infection

et al. 2015

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The aim of this study was to evaluate the effects of resveratrol (RSV) in its free or complexed (cyclodextrin) forms in association with diminazene aceturate (DA), as an attempt to reduce the toxic effects of this standard chemotherapy on renal and hepatic samples of mice experimentally infected with T. evansi. In this sense, this experiment was designed in order to test the ability of RSV, in its free or complexed forms, on reducing the damage on lipid membrane and protein oxidation, effects usually observed by using DA. Both forms of RSV were used at 100 mg kg −1 , during seven consecutive days. As a result, it was observed that, on renal and hepatic samples, RSV in its free form, when combined with DA, was able to inhibit the increase of thiobarbituric acid reactive species levels. DA caused a decrease in the hepatic levels of advanced oxidation protein products, suggesting changes in protein synthesis; however, when it was combined with RSV, this deleterious effect was not observed. It demonstrates that RSV was able to provide some grade of protection against protein oxidation. Therefore, RSV may represent an alternative in reducing the toxic effect caused by chemotherapy in treating of T. evansi infection.

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Trypanosoma evansi: Effect of experimental infection on the osmotic fragility, lipid peroxidation and calcium-ATPase activity of rat red blood cells

Cited by 23 publications

References 22 publications

Evolving anti-disease strategies from biochemical pathogenesis of african trypanosomiasis

Evolving anti-disease strategies from biochemical pathogenesis of african trypanosomiasis

Influence of extracellular media’s ionic strength on the osmotic stability of Sahel goat erythrocytes

Combination of diminazene aceturate and resveratrol reduces the toxic effects of chemotherapy in treating Trypanosoma evansi infection

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