Evolving anti-disease strategies from biochemical pathogenesis of african trypanosomiasis

Igbokwe, I.O.

doi:10.15406/acp.2018.03.00048

Cited by 4 publications

(1 citation statement)

References 84 publications

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“…Trypanosomes are blood parasites that infect wide range of livestock 1 . Diseases caused by trypanosomes include blood dyscrasias (anemia, leukopenia, thrombocytopenia), organ damage, and inflammation 1 . Eleven different species of pathogenic trypanosomes have been studied in Africa 2 .…”

Section: Introductionmentioning

confidence: 99%

Biochemical and genotyping analyses of camels (Camelus dromedaries) trypanosomiasis in North Africa

Darwish

Sharaf

Gaouar

et al. 2023

Sci Rep

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Camels are considered an important food source in North Africa. Trypanosomiasis in camels is a life-threatening disease that causes severe economic losses in milk and meat production. Therefore, the objective of this study was to determine the trypanosome genotypes in the North African region. Trypanosome infection rates were determined by microscopic examination of blood smears and polymerase chain reaction (PCR). In addition, total antioxidant capacity (TAC), lipid peroxides (MDA), reduced glutathione (GSH), superoxide dismutase (SOD) and catalase (CAT) were determined in erythrocyte lysate. Furthermore, 18S amplicon sequencing was used to barcode and characterizes the genetic diversity of trypanosome genotypes in camel blood. In addition to Trypanosoma, Babesia and Thelieria were also detected in the blood samples. PCR showed that the trypanosome infection rate was higher in Algerian samples (25.7%) than in Egyptian samples (7.2%). Parameters such as MDA, GSH, SOD and CAT had significantly increased in camels infected with trypanosomes compared to uninfected control animals, while TAC level was not significantly changed. The results of relative amplicon abundance showed that the range of trypanosome infection was higher in Egypt than in Algeria. Moreover, phylogenetic analysis showed that the Trypanosoma sequences of Egyptian and Algerian camels are related to Trypanosoma evansi. Unexpectedly, diversity within T. evansi was higher in Egyptian camels than in Algerian camels. We present here the first molecular report providing a picture of trypanosomiasis in camels, covering wide geographical areas in Egypt and Algeria.

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Section: Introductionmentioning

confidence: 99%

Biochemical and genotyping analyses of camels (Camelus dromedaries) trypanosomiasis in North Africa

Darwish

Sharaf

Gaouar

et al. 2023

Sci Rep

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β-Sitosterol could serve as a dual inhibitor of Trypanosoma congolense sialidase and phospholipase A2: in vitro kinetic analyses and molecular dynamic simulations

et al. 2022

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Evaluation of biochemical indices in indigenous cattle breeds and crossbred genotypes under natural trypanosome challenge

Nwachukwu,

Ogbu,

Kalla

et al. 2023

Preprint

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Breeding for trypanotolerance could be a more sustainable control strategy against African animal trypanosomiasis. This study examined the biochemical indices of crossbred/composite cattle, N’dama and WF breeds under natural trypanosome challenge to assess trypanotolerance capacity. Serum total protein, albumin, globulin, ALT, AST, ALP, bilirubin, creatinine and urea were compared among cattle genotypes, genotypic groups, anaemic and non-anaemic, and parasitaemic and aparasitaemic groups. Non-anaemic cattle had higher (p < 0.05) total protein and albumin compared to anaemic cattle. Globulin, albumin/globulin, ALT, ALP, and creatinine did not differ significantly between cattle genotypes but AST was highest in anaemic NDxWF (38.50 ± 6.19 U/l) while indirect bilirubin and urea were highest in non-anaemic 75NDx25WF. Aparasitaemic cattle had higher total protein, albumin and ALP but lower creatinine than parasitaemic cattle. AST and total bilirubin were highest in ND(GUxWFxMU) (33.18 ± 1.78 U/l) and ND(GUxWF) (0.30 ± 0.03 mg/dl), respectively and least in parasitaemic WF (24.63 ± 3.57 U/l) and NDxWF (0.19 ± 0.03 mg/dl), respectively. Creatinine, indirect bilirubin and urea were highest in 75NDx25WF (1.23 ± 0.26 mg/dl, 0.23 ± 0.03 mg/dl, and 39.68 ± 3.05 mg/dl, respectively) and lowest in ND (0.28 ± 0.10 mg/dl), parasitaemic WF (0.11 ± 0.03mg/dl), and ND(BOxWF) (22.84 ± 3.05 mg/dl), respectively. Crossbred/composite cattle and N’Dama had higher serum total protein, albumin, AST, total and indirect bilirubin, but lower creatinine compared to WF cattle indicating better body nutrient reserves, lower oxidative stress challenge and stronger trypanotolerance capacity.

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Evolving anti-disease strategies from biochemical pathogenesis of african trypanosomiasis

Cited by 4 publications

References 84 publications

Biochemical and genotyping analyses of camels (Camelus dromedaries) trypanosomiasis in North Africa

Biochemical and genotyping analyses of camels (Camelus dromedaries) trypanosomiasis in North Africa

β-Sitosterol could serve as a dual inhibitor of Trypanosoma congolense sialidase and phospholipase A2: in vitro kinetic analyses and molecular dynamic simulations

Evaluation of biochemical indices in indigenous cattle breeds and crossbred genotypes under natural trypanosome challenge

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