Influence of extracellular media’s ionic strength on the osmotic stability of Sahel goat erythrocytes

Abstract

In this study, the variation in osmotic stability of caprine erythrocytes was linked to ionic strength of the suspending extracellular media which seemed to exert an influence through transmembrane ion fluxes and regulatory volume changes in erythrocytes.

Phenotypic drift in osmotic fragility of Sahel goat erythrocytes associated with variability of median fragility

“…(Oyewale, 1991;Oyewale, 1993;Oyewale et al, 1997) and Sahel goats (Igbokwe & Igbokwe, 2015), 8 g/L in Red Sokoto (RS) goats (Habibu et al, 2014), and 6-8 g/L in RS goats during rest and after loading and transportation stress (Minka & Ayo, 2010). Some of these MEF values were read from published fragiligrams where there was no haemolysis in isotonic saline (Oyewale, 1991;Oyewale, 1993;Oyewale et al, 1997;Igbokwe & Igbokwe, 2015) in contrast to where there was isotonic haemolysis of about 20% (Habibu et al, 2014) or 20-40% (Minka & Ayo, 2010). An isotonic haemolysis may be an artefact arising from using blood samples with extracellular haemoglobin due to in vitro or in vivo haemolysis as shown in the increase of the mean corpuscular haemoglobin concentration (MCHC) to 63.7 ± 2.8 g/dL (Minka & Ayo, 2010) from a lower reference mean (32.3-39.6 g/dL) for goats (Byers & Kramer, 2010).…”

“…A stock solution of buffered saline was prepared as follows: 90.0 g sodium chloride (NaCl), 13.65 g disodium hydrogen phosphate (Na 2 HPO) (BDH, England), 2.34 g sodium dihydrogen phosphate (NaH 2 PO 4 ) (BDH, England), all made up to 1 L with deionized distilled water, giving a stock solution of 10% NaCl (Ochei & Kolhatkar, 2007). The working solution of 1% NaCl was prepared by dilution of the stock solution from which other lower concentrations of saline were prepared as earlier described (Igbokwe & Igbokwe, 2015). Each saline solution at various dilutions (5 mL) or deionised distilled water (5 mL) in a tube had an aliquot of a blood sample (5 μL) added to it, mixed by inversion and allowed to stand for 30 min under room temperature (35-38 °C).…”

“…Normal values of MEF in goats are not available apart from those estimated from fragiligrams in reports of some controlled experiments with disparities in data presentations (Oyewale, 1991;Oyewale, 1993;Oyewale et al, 1997;Minka & Ayo, 2010;Habibu et al, 2014;Igbokwe & Igbokwe, 2015). Phenotypic variations in EOF may exist in Sahel goat populations under physiological conditions as was previously reported with respect to erythrocyte glutathione concentrations (Igbokwe et al, 1998).…”

“…Decreased stability (increase in EOF) shifts MEF to higher saline concentration leading to a drift of the fragiligram from sigmoidal to hyperbolic curve (Igbokwe & Igbokwe, 2015). Normal values of MEF in goats are not available apart from those estimated from fragiligrams in reports of some controlled experiments with disparities in data presentations (Oyewale, 1991;Oyewale, 1993;Oyewale et al, 1997;Minka & Ayo, 2010;Habibu et al, 2014;Igbokwe & Igbokwe, 2015).…”

“…Evaluation of the association of EOF with variations in the values of erythrocyte size and packed cell volume (PCV) in goats is yet to be evaluated to ascertain the correlation of these physiological parameters as a means of understanding their dynamic inter-relationships. In this study, phenotypic drift of EOF curves based on the variability of MEF in Sahel goats, under semi-intensive management, was investigated and the correlative strength of erythrocyte size and the associated PCV as modifiers of EOF phenotype was evaluated in order to establish appropriate baseline reference data for use in understanding erythrocyte membrane defects under clinical and experimental conditions (Igbokwe & Igbokwe, 2015).…”