Southeastern Brazilian Mikania (Asteraceae) species were evaluated for trypanocidal activity against the trypomastigote forms of Trypanosoma cruzi at a concentration of 4000 mg=mL. Fourteen extracts were examined for in vitro trypanocidal properties. Of total extracts, 92.9% (13 extracts) exhibited trypanocidal effects. The dichloromethane extract of Mikania camporum B. Robinson and the methanol extract of Mikania micrantha H. B. K. caused 100% lysis of the parasites.