Troxerutin protect sperm, seminiferous epithelium and pituitary-gonadal axis from torsion-detorsion injury: An experimental study

Abstract

Background:Troxerutin is a flavonoid antioxidant that protect different organ against damage caused by ischemia-reperfusion.Objective:The aim of this study was to evaluate the effect of troxerutin in reducing the damages caused by ischemia-reperfusion in rat's testis.Materials and Methods: 40 Male Wistar rats (2 month old) were divide to four groups (n=10). Group1 (sham), Group 2 (control, ischemia-reperfusion (I/R) without treatment), Group 3 (I/R+150 mg/kg of troxerutin), and group 4 (I/R+20 mg/kg of vitamin C). Treatment of group 3 and group 4 during torsion (twists 720 counter clock at 90 min) followed by 50 days detorsion. After 50 days, blood samples were collected and rats in all study groups were killed and their testes were removed, and fixed with Bouin’s solution. Testis was stained with hematoxylin and eosin dye and the level of testosterone, luteinizing hormone (LH) and follicle-stimulating hormone (FSH) were measured with ELISEA methods. TUNEL was employed to detect apoptosis. Epididymis caudal part was removed and total sperm count was determined. Johnson techniques were used for assessment of seminiferous tubules quality.Results: Troxerutin treated group has higher Johnson score’s (p≤0.001), antiapoptotic properties (p≤0.001), sperm count (p=0.065), and higher LH (p≤0.001), FSH (p≤0.001) and testosterone (p=0.002) levels than control group. Vitamin C treated group showed increase level of testosterone but didn’t show significant differences on the number of apoptotic cells, Johnson scores, LH, FSH and sperm count than control group.Conclusion:Troxerutin has protective effects on testicular torsion induced injury and can ameliorate spermatogenesis in the torsion-detorsion models.

Troxerutin Prevents 5-Fluorouracil Induced Morphological Changes in the Intestinal Mucosa: Role of Cyclooxygenase-2 Pathway

“…Another study has shown that TRX is capable of reversing mastocytosis [18]. According to Kheirollahi et al [53], flavonoids reverse tissue damage by preventing neutrophil and mast cell degranulation, which corroborates with the findings obtained in this study.…”

Investigation of the effect of silymarin on oxidative DNA damage and inflammatory markers in ischemia/reperfusion injury following experimental testicular torsion/detorsion in rats

“…Serum testosterone levels in the torsion/detorsion groups were found to be significantly decreased when compared to the control group. The decrease in testosterone has been similarly reported in previous testicular ischemia studies (Abdelzaher et al, 2020;Shakoohi et al, 2018;Kheirollahi et al, 2018). The decrease in testosterone may be associated with damage to the Leydig cells (Benny et al, 2019).…”

Sinapic acid reduces ischemia/reperfusion injury due to testicular torsion/detorsion in rats

“…Therefore, studies using experimental models for the treatment of testicular torsion are still being conducted. Many protective agents have been researched over the years (Bodur et al., 2016; Kheirollahi et al., 2018; Semercioz et al., 2017). However, despite all the research conducted, a treatment method with easy clinical adaptation and proven benefits has yet to be discovered.…”