Investigation of the effect of silymarin on oxidative DNA damage
and inflammatory markers in ischemia/reperfusion injury
following experimental testicular torsion/detorsion in rats

Belhan, Saadet; Yıldırım, Serkan; Kayikci, Caner; Kömüroğlu, Ahmet Ufuk; Özdek, Uğur; Kuşçu, Yağmur

doi:10.3906/zoo-2103-5

Cited by 2 publications

(1 citation statement)

References 34 publications

(46 reference statements)

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“…Our results are in agreement with other studies which have reported the reduction of serum testosterone levels in the torsion/detorsion process. Belhan et al reported in their study that testicular ischemia/reperfusion led to 7 Andrologia testis tissue damage and reduction in serum testosterone level [32].…”

Section: Discussionmentioning

confidence: 94%

The Flavonoid Chrysin Protects against Testicular Apoptosis Induced by Torsion/Detorsion in Adult Rats

et al. 2023

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In the present work, we examined the beneficial efficacy of chrysin as a natural flavonoid on testicular torsion/detorsion damages among adult male rats. Forty-eight male Wistar rats were randomly allocated into six groups: sham, torsion/detorsion (TD) group, TDC30 group in which TD process was applied and treated with chrysin (30 mg/kg), TDC50 group in which TD process was applied and treated with chrysin (50 mg/kg), HC30 group in which animals were treated with chrysin (30 mg/kg), and HC50 group in which rats were treated with chrysin (50 mg/kg). Serum samples were tested for testosterone, luteinizing hormone (LH), and follicle-stimulating hormone (FSH) levels. The sperm parameters, histopathological analysis, and expressions of apoptosis-related genes were examined among different groups. Superoxide dismutase (SOD), glutathione peroxidase (GPx), and malondialdehyde (MDA) levels in the testicles and serum were measured. Serum levels of SOD, GPx, FSH, LH, and “Bax” expression significantly increased in the TD group compared to the sham group ( P < 0.05 ). Sperm parameters, serum testosterone level, Johnson’s scores, seminiferous tubule diameter, the height of the germinal epithelium (HE) measurements, and expression of “Bcl2” meaningfully decreased ( P < 0.05 ). Administration of chrysin impeded ischemia/reperfusion damages in testis tissue and improved sperm quality. The result of our study indicates that treatment with chrysin can protect testis tissue from ischemia/reperfusion damages induced by TD procedure.

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Section: Discussionmentioning

confidence: 94%

The Flavonoid Chrysin Protects against Testicular Apoptosis Induced by Torsion/Detorsion in Adult Rats

et al. 2023

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The Effect of Chrysin on Oxidative Stress in the Ovarian Tissue of Aspirin Administered Rats

2021

JSTR

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Aspirin is a medication with anti-inflammatory and antioxidant properties. Chrysin is a natural flavonoid that has been extensively investigated for its significant biological impacts. In this study, we aimed to investigate the protective effect of chrysin against aspirin-induced oxidative damage in rat ovarian tissue. Forty female albino rats were used in the study. Rats were randomly divided into 5 groups. Group1 (control group): No medication was administered to the rats in this group. Group 2 (ASA group): 1 mg/kg aspirin was administered to rats in this group by oral gavage for 28 days. Group 3 (ASA+CH): 1 mg/kg aspirin and 50 mg/kg CH were administered to rats in this group by oral gavage for 28 days. Group 4 (CH): 50 mg/kg CH was administered by oral gavage to the rats in this group for 28 days. Group 5 (olive oil): 1 ml/kg of olive oil was administered orally to the rats in this group. At the end of the 28-day trial, ovarian tissues were taken under anesthesia, after the rats fasted for one night. The supernatant was obtained by homogenizing the ovarian tissues, and Malondialdehyde (MDA), Advanced Oxidation Protein Products (AOPP), Total sulfhydryl (T-SH) level, and catalase (CAT) activity were quantified spectrometrically from the supernatant. Ovarian tissue MDA and AOPP levels in the ASA group were determined to be significantly higher than in the control group (p<0.05). Ovarian tissue MDA level in the ASA+CH group was lower compared to the ASA group, but the difference between the two groups was not significant (p>0.05). The AOPP level of the ASA+CH group was found to be significantly lower than that of the ASA group (p<0.05). T-SH level of the ASA group was significantly lower than the control group (p<0.05). No significant difference was determined between T-SH levels of the ASA and AS+CH group (p>0.05). CAT activity in the ASA group was lower than in the control group, but the decrease was not significant (p>0.05). Ovarian tissue CAT activity was found to be significantly higher in the ASA+CH group, compared to the control and ASA groups (p<0.05). ASA administration causes an increase in oxidative stress markers from the ovarian tissue, whereas it causes a decrease in antioxidants. By reducing this effect of aspirin, CH could increase antioxidant levels and reduce oxidative stress

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Investigation of the effect of silymarin on oxidative DNA damage and inflammatory markers in ischemia/reperfusion injury following experimental testicular torsion/detorsion in rats

Cited by 2 publications

References 34 publications

The Flavonoid Chrysin Protects against Testicular Apoptosis Induced by Torsion/Detorsion in Adult Rats

The Flavonoid Chrysin Protects against Testicular Apoptosis Induced by Torsion/Detorsion in Adult Rats

The Effect of Chrysin on Oxidative Stress in the Ovarian Tissue of Aspirin Administered Rats

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