TROTS: A computer graphics system for three-dimensional reconstruction from serial sections

Veen, Arthur H.; Peachey, Lee D.

doi:10.1016/0097-8493(77)90035-8

Cited by 40 publications

(11 citation statements)

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“…Some of these systems permit the threedimensional reconstruction of the ultrastructure of individual neurons or their processes from serial electron micrographs (Levinthal and Ware, 1972;Willey et al, 1973;Levinthal et al, 1974;Rakic et al, 1974;Ward et al, 1975;White et al, 1976;Lewis and Hodgkin, 1977;Mazziotta and Hamilton, 1977;Veen and Peachey, 1977;Shantz and McCann, 1978;Street and Mize, 1983). Another group of computer-aided systems permit the analysis of intracellularly stained (Reddy et al, 1973) or Golgi-impregnated neurons, their processes (Coleman et al, 1973;Standler and Bernstein, 1982), and their graphic reconstruction and display (Wann et al, 1973;Llinas and Hillman, 1975;Capowski and Rethelyi, 1978;Capowski and Sedivec, 1981).…”

Section: Discussionmentioning

confidence: 98%

Computer‐aided reconstructive morphology of the baboon abducens nucleus

1985

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Our previous effort at reconstructive morphology included the marriage of the horseradish peroxidase (HRP) neurohistochemical method to a Lucite plate reconstruction technique. Limitations imposed by this combination of methods has led us to develop a computer-based system that utilizes image-processing techniques and the data obtained from HRP-processed serial light microscope sections. Labeled neurons of the baboon abducens nucleus were identified by HRP conjugated to wheat ger agglutinin. Using the resulting serial sections and a unique imaging process involving a pattern recognition algorithm, our computer-based system automatically differentiates neuronal from nonneuronal features, delineates the surface boundaries of the neuronal population, and then assembles these serial sections into a solid three-dimensional structure that can be rotated and further analyzed. A computer-generated solid model of this neuronal population has been reconstructed and reproduced in a two-dimensional publishable format. It is anticipated that with further development this system will be able to utilize data from the same specimen to study spatial relations through three-dimensional reconstruction, as well as to study the quantitative morphology of a neuronal population. Other computer-aided systems are noted, as are the advantages and shortcomings of the present method.

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Section: Discussionmentioning

confidence: 98%

Computer‐aided reconstructive morphology of the baboon abducens nucleus

1985

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“…Furthermore, computer programs have been recently developed [Veen and Peachey, 1977] to facilitate the three-dimensional reconstruction of struc tures from the profiles of serial sections. Perhaps a better elucidation of SR structure would be obtained from serial thin section ing of tissue stained with the SR-selective methods [Peachey et al, 1974;Sommer and Waugh, 1976;Forbes et al, 1977], Finally, comparing the results of our serial-section study of SR with those obtained by means of high-voltage electron microscopy [Peachey et al, 1974;Peachey, 1976;Sommer and Waugh, 1976], it is appreciated that with the use of thin sec tions, higher resolution is obtained.…”

Section: Discussionmentioning

confidence: 99%

Sarcoplasmic reticulum in Purkinje cells of the goat heart

Núñez‐Durán¹

1980

Cells Tissues Organs

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A three-dimensional study of the sarcoplasmic reticulum (SR) of Purkinje cells of the goat heart was carried out with the use of serial thin sectioning. Two interconnected forms of SR were distinguished: myofibrillar SR and junctional SR (JSR). An extensive network of tubules lying outside as well as inside the myofibrils, constitutes the myofibrilar SR. The JSR, consists of vesicles, tubules and flattened cisternae. A three-dimensional model of a JSR cisterna was built showing its connection with both the myofibrillar SR and the sarcolemma where it forms a peripheral coupling. The potentiality and limitations of the serial-section technique for the study of the SR are discussed in comparison with other recently developed methods.

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“…To record either minimally (or non-) overlapped images or sets of images capable of depth discrimination, it is essential to tilt the specimen accurately t o any orientation relative to the electron beam (Turner, 1981). Stage performance is especially important for serialsection reconstruction with (Marko et al, 1986(Marko et al, 1987(Marko et al, , 1988 or without (Parsons et al, 1985;Rieder, 1981;Veen and Peachey, 1977;Young et al, 1987) stereoprofiling and for tomographic reconstructions McEwen et al, 1986McEwen et al, , 1987.…”

Section: Introductionmentioning

confidence: 98%

Optimum specimen positioning in the electron microscope using a double‐tilt stage

Turner

Rieder

Collins

et al. 1989

J. Elec. Microsc. Tech.

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Optimal imaging of complex structures requires proper alignment relative to the optic axis of the electron microscope. This is especially important for high-voltage and intermediate-voltage microscopes, which form an in-focus image throughout the entire thickness of the object. As a result, structures at different specimen heights form overlapping and confused images that severely curtail the usefulness of these instruments. The work described here provides a generalized, flexible method for optimizing specimen orientation and eliminating or limiting image overlap by means of a commonly used double-tilt stage. Analysis of the motion about the two axes provides accurate tilting for any azimuthal direction whether or not it corresponds to a mechanical axis of the stage. An object can be positioned to minimize image overlap, to record stereopairs for any parallax axis, and to record three-dimensional data sets by the conical collection geometry. Images of muscle paracrystals are shown after tilting about an axis perpendicular to a symmetry direction. The tilted image displays higher-order symmetry, which is altered by changes of one degree. Precision double-tilting for optimizing stereopairs is shown for a desmosome recorded using different parallax axes and pretilts. A tomographic conical data-collection scheme is demonstrated by imaging a microtubule axoneme for a specific cone half-angle and arbitrary azimuthal angles.

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TROTS: A computer graphics system for three-dimensional reconstruction from serial sections

Cited by 40 publications

References 1 publication

Computer‐aided reconstructive morphology of the baboon abducens nucleus

Computer‐aided reconstructive morphology of the baboon abducens nucleus

Sarcoplasmic reticulum in Purkinje cells of the goat heart

Optimum specimen positioning in the electron microscope using a double‐tilt stage

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