Triple tumor markers assay based on carbon–gold nanocomposite

Teng, Xu; Liu, Na; Yuan, Jing; Ma, Zhanfang

doi:10.1016/j.bios.2015.03.036

Cited by 71 publications

(29 citation statements)

References 29 publications

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“…This makes carbon nanosphere an ideal material in the application of immunoprobes for the simultaneous multiplexed immunoassay. In our group, carbon nanosphere was obtained by the microwave-assisted carbonization of glucose in several minutes [66]. AuNPs were immobilized through the in situ reduction method by dispersion of the carbon nanosphere in the presence of HAuCl 4 .…”

Section: Immunoprobesmentioning

confidence: 99%

Design of immunoprobes for electrochemical multiplexed tumor marker detection

Ma¹,

Liu

2015

Expert Review of Molecular Diagnostics

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Many approaches have been developed for simultaneous detection of multiple tumor markers. Among these approaches, the electrochemical immunoassay has the advantage of high sensitivity and specificity and could be easily expanded into multiplex detection platform. For the simultaneous multianalyte electrochemical immunosensor, performance is closely related with the characteristics of the immunoprobes and substrate. In order to construct a multilabeled immunoprobe platform, the most important issue is how to discriminate each signal for each analyte from the multiple antigen-antibody reactions. Currently, enzyme-based, noble metal nanomaterials, carbonmaterials and polymer-based nanomaterial immunoprobes have been used for dual- or three-analyte detections. However, there are still some challenges in developing sensitive method to detect three or more tumor markers owing to the lack of redox-active species that can produce three or more distinctive peaks. Additionally, for the immunosensing substrate, good conductivity, high specific surface area and good biocompatibility are further necessities.

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Section: Immunoprobesmentioning

confidence: 99%

Design of immunoprobes for electrochemical multiplexed tumor marker detection

Ma¹,

Liu

2015

Expert Review of Molecular Diagnostics

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“…For cancer patients, the early detection may contribute to early diagnosis and treatment of malignancies and improve chances of survival 4 (Arya and Bhansali 2011;Chikkaveeraiah et al 2012). Tumor markers are the important indices of cancer; therefore, a significant focus on the detection of tumor markers has initiated to provide early diagnosis (Rich and Singal 2014; Xu et al 2015). Immunoassays, based on antigen-antibody specific reactions, have been developed due to their specificity, sensitivity, and precision.…”

Section: Introductionmentioning

confidence: 99%

Novel Electrochemical Immunoassay for Carcinoembryonic Antigen Using Ferrocenyl Polymer Nanospheres

Zhao

Zhang

et al. 2016

Analytical Letters

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A new electrochemical immunoassay based on a sandwich format was developed for carcino embryonie antige using ferrocenyl polymer nanospheres as the recognition elements. Ferrocenyl nanospheres were synthesized by coordination polymerization and characterized by transmission Downloaded by [University of California Santa Barbara] at 02:40 15 June 20163 electron microscopy, energy dispersive X-ray spectrometry, and infrared spectroscopy.Monoclonal carcino embryonie antibodies were immobilized on a nanogold-modified gold electrode to enhance the sensitivity. Polyclonal carcino embryonie antibodies were conjugated on ferrocenyl polymer nanospheres. Under the optimal conditions, the electrochemical signal increased with the concentration of the analyte from 0.01 to 50 ng mL 1 with a limit of detection equal to 0.004 ng mL 1 . The immunosensor provided acceptable reproducibility with coefficients of variation below 10%.

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“…An electrochemical platform prepared by modification of a GCE with rGO and the ionic liquid 1-aminopropyl-3-methylimidazolium chloride was used for immobilization of the capture antibodies. A sandwich-type immunoassay was performed which allowed simultaneous detection of the three antigens by SWV with LOD values at the pg·mL −1 level [86].…”

Section: Other Carbon Nanomaterials Used As Carriers For Signal Elemementioning

confidence: 99%

Carbon Nanostructures for Tagging in Electrochemical Biosensing: A Review

Yáñez‐Sedeño

Campuzano

Pingarrón

2017

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Growing demand for developing ultrasensitive electrochemical bioassays has led to the design of numerous signal amplification strategies. In this context, carbon-based nanomaterials have been demonstrated to be excellent tags for greatly amplifying the transduction of recognition events and simplifying the protocols used in electrochemical biosensing. This relevant role is due to the carbon-nanomaterials' large surface area, excellent biological compatibility and ease functionalization and, in some cases, intrinsic electrochemistry. These carbon-based nanomaterials involve well-known carbon nanotubes (CNTs) and graphene as well as the more recent use of other carbon nanoforms. This paper briefly discusses the advantages of using carbon nanostructures and their hybrid nanocomposites for amplification through tagging in electrochemical biosensing platforms and provides an updated overview of some selected examples making use of labels involving carbon nanomaterials, acting both as carriers for signal elements and as electrochemical tracers, applied to the electrochemical biosensing of relevant (bio)markers.

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Triple tumor markers assay based on carbon–gold nanocomposite

Cited by 71 publications

References 29 publications

Design of immunoprobes for electrochemical multiplexed tumor marker detection

Design of immunoprobes for electrochemical multiplexed tumor marker detection

Novel Electrochemical Immunoassay for Carcinoembryonic Antigen Using Ferrocenyl Polymer Nanospheres

Carbon Nanostructures for Tagging in Electrochemical Biosensing: A Review

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