Tripanosomatídeos similares a Trypanosoma theileri no carrapato dos bovinos Boophilus microplus

Martins, João Ricardo; Leite, Romário Cerqueira; Doyle, Rovaina Laureano

doi:10.1590/s1984-29612008000200010

Cited by 19 publications

(17 citation statements)

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“…However, the occurrence of epimastigote forms of a trypanosome in haemolymph of this tick species has also been reported by Ribeiro et al (1988) and Martins et al (2008) in Southern and central Brazil, respectively, and by Gaido et al (1989) in Argentina. Morphological evidence led the authors to suspect T. theileri, as this species was described as non-pathogenic in cattle and usually transmitted by horseflies.…”

Section: Discussionsupporting

confidence: 67%

Trypanosoma rhipicephalis sp. nov. (Protozoa: Kinetoplastida) isolated from Rhipicephalus microplus (Acari: Ixodidae) ticks in Rio de Janeiro, Brazil

et al. 2018

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Parasites of the genusTrypanosomaare unicellular flagellated microorganisms of theTrypanosomatidae. This study describes an isolate of the genusTrypanosomanaturally infectingRhipicephalus microplusticks, characterized through molecular, morphological and biological analysis. Trypanosome cultures, designated strain P1RJ, were obtained by isolation fromR. microplushaemolymph in cultures of the tick cell line IDE8. After isolation, strain P1RJ grew well axenically in L15B medium at temperatures of 30, 32 and 34 °C. The new trypanosome remained stable in axenic culture over 14 passages in L15B at 30 °C and was successfully cryopreserved and resuscitated. Morphometric analysis was performed on randomly selected developmental forms. 18S rRNA and 24Sα rDNA sequence analyses confirmed that strain P1RJ is a new species of the genusTrypanosoma. The nucleotide sequences described were submitted to Genbank. Pathogenicity, involvement in vertebrate hosts, epidemiology, developmental cycle and transmission mechanisms of strain P1RJ are still unknown. Therefore, more studies will be necessary to determine life cycle aspects of this trypanosome, for which we propose the nameTrypanosoma rhipicephalissp. nov.

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Section: Discussionsupporting

confidence: 67%

Trypanosoma rhipicephalis sp. nov. (Protozoa: Kinetoplastida) isolated from Rhipicephalus microplus (Acari: Ixodidae) ticks in Rio de Janeiro, Brazil

et al. 2018

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“…considering the most widely used genetic marker of piroplasms (18S rRNA gene), relevant sequences are only published for T. annulata, and only from one study in Pakistan: Khan et al 2013]. In addition, tick-infecting trypanosomes have also been reported from the above tick species (from H. anatolicum in Africa: Latif et al 2004; from R. microplus in South America: Martins et al 2008), but similar data have not been published from the region of Pakistan.…”

Section: Introductionmentioning

confidence: 99%

Genetic diversity, piroplasms and trypanosomes in Rhipicephalus microplus and Hyalomma anatolicum collected from cattle in northern Pakistan

et al. 2019

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The two most important tick species in Pakistan are Rhipicephalus microplus and Hyalomma anatolicum. When associated with cattle, these have one or three host life cycles, respectively, with potential implications for their population genetics and for their vector role in the transmission of pathogens. To compare the two tick species in this context with molecular-phylogenetic methods, during the present study 123 ticks were collected from cattle in northern Pakistan. Two mitochondrial markers of 36 ticks were molecularly analyzed. All 11 R. microplus specimens had identical cox1 haplotypes, whereas the 25 H. anatolicum specimens had nine cox1 haplotypes. The latter belonged to two distinct phylogenetic lineages with high support. However, in the 16S rRNA gene these differences were less evident. Among the 113 ticks molecularly analyzed for tick-borne protozoa, the sequence of Babesia occultans was successfully amplified from two specimens of H. anatolicum. Theileria annulata was present in both R. microplus (10.4%) and H. anatolicum (27.3%), with significantly higher prevalence rate in the latter species. Only one tick, a H. anatolicum female, was positive in the PCR detecting Trypanosoma spp. Sequencing revealed the presence of a new genotype, with the closest phylogenetic relationship to stercorarian trypanosomes (in particular, to a tick-associated Trypanosoma sp. from Japan). In conclusion, the above differences between R. microplus and H. anatolicum may be partly related to their life cycles involving one host or three hosts, respectively. Among the others, host switching (reducing chances of inbreeding) and shorter periods spent on-host (reducing gene flow between cattle herds) are supposed to be important drivers of cox1 gene diversification in case of H. anatolicum as a three host tick species. These results highlight the importance of studying differences in intraspecific genetic diversity and piroplasm burdens between one host and three host ticks in the local scale. In addition, a Trypanosoma sp. molecularly identified in H. anatolicum is reported here for the first time from South Asia, deserving further evaluation concerning its host and vector species. Keywords Cox1 • 16S rRNA • One host tick • Three host tick • Theileria annulata • Babesia occultans

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“…Although a molecular confirmation was not performed, the protozoans that were found were morphologically very similar to Trypanosoma theileri, which is a cosmopolitan parasite common in mammals of the order Artiodactyla that has been found in cattle from Mato Grosso do Sul 24 .…”

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confidence: 96%

Epidemiological factors related to the transmission risk of Trypanosoma cruzi in a Quilombola community, State of Mato Grosso do Sul, Brazil

Cominetti

Andreotti

Oshiro

et al. 2011

Rev. Soc. Bras. Med. Trop.

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Epidemiological factors related to the transmission risk of Trypanosoma cruzi in a Quilombola community, State of Mato Grosso do Sul, BrazilThe parasitological tests showed flagellates in an opossum and two cattle. The molecular tests showed DNA from T. cruzi in an opossum and a pig. Triatoma sordida was the only triatomine species found in the community, and it colonized households (four specimens) and the surrounding areas (124 specimens). Twentythree specimens tested positive for flagellates, which were subsequently identified as T. cruzi by PCR. Conclusions: Data analysis demonstrated that T. cruzi has a peridomestic life cycle that involves both domestic and wild mammals.

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Tripanosomatídeos similares a Trypanosoma theileri no carrapato dos bovinos Boophilus microplus

Abstract: Figure 1. Epymastigotes forms of Trypanosoma sp. in hemolymph smears of Boophilus microplus stained by Giemsa (1000x).

Cited by 19 publications

References 1 publication

Trypanosoma rhipicephalis sp. nov. (Protozoa: Kinetoplastida) isolated from Rhipicephalus microplus (Acari: Ixodidae) ticks in Rio de Janeiro, Brazil

Trypanosoma rhipicephalis sp. nov. (Protozoa: Kinetoplastida) isolated from Rhipicephalus microplus (Acari: Ixodidae) ticks in Rio de Janeiro, Brazil

Genetic diversity, piroplasms and trypanosomes in Rhipicephalus microplus and Hyalomma anatolicum collected from cattle in northern Pakistan

Epidemiological factors related to the transmission risk of Trypanosoma cruzi in a Quilombola community, State of Mato Grosso do Sul, Brazil

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