“…The elution order is associated to an increasing unsaturation degree and position/configuration of fatty acid DBs [4,10]. Several analytical methodologies related to analysis of TGs; such as thin-layer chromatography (TLC) [11,12], reservedphase HPLC (RP-HPLC) [13][14][15][16], non-aqueous RP-HPLC [17,18], normal-phase HPLC (NP-HPLC) [19], high-temperature gas-liquid chromatography (HT-GLC) [20][21][22] and Ag + -HPLC have been also reported. GLC technique has some problems due to the fact that the trans-esterification of polyunsaturated TAGs isn't always quantitative and there is less information about whole composition of TAGs [23,24].…”