Central composite design and response surface methodology for the optimization of Ag+-HPLC/ELSD method for triglyceride profiling

Arslan, Fatma Nur; Kara, Hüseyin

doi:10.1007/s11694-016-9462-5

Cited by 11 publications

(9 citation statements)

References 38 publications

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“…Randomization of the experimental variables was assumed to be achieved through the generated experimental runs. The predictor model for the response variables was generated using the second‐degree polynomial equation with the consideration of the full quadratic model coefficients and interaction factors as shown in Equation 3 (Arslan & Kara, 2017; Yabalak et al., 2019).

y = a + β_{1} x_{1} + β_{2} x_{2} + β_{3} x_{3} + β_{4} x_{1} x_{2} + β_{5} x_{1} x_{3} + β_{6} x_{2} x_{3} + β_{7} x_{1}^{2} + β_{8} x_{2}^{2} + β_{9} x_{3}^{2} + ε,

…”

Section: Methodsmentioning

confidence: 99%

Comparative profiling of lactic acid bacteria isolates in optimized and spontaneous fermentation of cowpea leaves

Owade

Abong

Okoth

et al. 2021

Food Science & Nutrition

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In as much as spontaneous fermentation of cowpea leaves enhances product diversification, the process is rather slow with poor product quality. Limited work has been undertaken to provide input toward standardization of the process and enhancing of product quality. The current study sought to evaluate the in‐process fermentative bacteria profile changes due to the effect of optimization of fermentation process of cowpea leaves. Lactic acid bacteria (LAB) isolates from spontaneous and optimized fermentation were characterized using biochemical tests, whereby optimization was done using the Response Surface Methodology model of the central composite design in the Design Expert Software. The RSM models accounted for 89% and 60% variability in the response variables of pH and titratable acidity, respectively (p < .001). Increasing the sugar concentration and period of fermentation significantly (p < .05) increased the titratable acidity, while reducing the pH. The optimal fermentation parameters were established as sugar and salt concentrations of 5% and 2%, respectively, 16 days of fermentation, pH of 3.8 and titratable acidity of 1.22% with a desirability of 0.859. Of the 13 identified LAB isolates, Lactobacillus brevis and Lactococcus lactis dominated the onset stage of spontaneous fermentation whereas only Lactobacillus brevis dominated the onset stage of optimized fermentation. Additionally, the final stage with the dominant isolates of L. plantarum was longer in the spontaneous fermentation process than in the optimized process. Evidently, optimizing the fermentation process resulted in increasing dominance by heterofermenters in the production of soured cowpea leaves, with the yielded product having enhanced acidity.

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y = a + β_{1} x_{1} + β_{2} x_{2} + β_{3} x_{3} + β_{4} x_{1} x_{2} + β_{5} x_{1} x_{3} + β_{6} x_{2} x_{3} + β_{7} x_{1}^{2} + β_{8} x_{2}^{2} + β_{9} x_{3}^{2} + ε,

…”

Section: Methodsmentioning

confidence: 99%

Comparative profiling of lactic acid bacteria isolates in optimized and spontaneous fermentation of cowpea leaves

Owade

Abong

Okoth

et al. 2021

Food Science & Nutrition

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“…In addition, the quality of the reduced model can also be verified based on the residuals vs. predicted plot (Figure 3c) that shows a random and even distribution above and below zero axis, suggesting that the constant variance is not present. Also, the predicted vs actual response plot of the reduced model (Figure 3d) follows a straight diagonal line, implying that the experimental responses are well predicted by the reduced quadratic model [14,26] …”

Section: Resultsmentioning

confidence: 70%

“…[11][12][13][14] RSM has been applied in the optimization of analytical procedures, such as the optimization of extraction, compounds separation, and chromatographic parameters. [13,14] In this study, ELSD operating conditions were optimized using experimental design approach by employing RSM technique for the analysis of frondoside A. Frondoside A is a monosulfated triterpenoid glycoside contained in the edible North Atlantic sea cucumber (Cucumaria frondose), a species that is commonly found in the North Atlantic coast of America. [15] Frondoside A exhibits a broad range of anti-cancer activities, such as inhibition of tumor growth, invasion, migration, metastases and angiogenesis, as well as induction of cellular apoptosis in diverse cancer types, including breast, lung, and pancreatic cancer.…”

Section: Introductionmentioning

confidence: 99%

“…The major advantages of RSM approach over OVAT have been proven in the optimization of various analytical processes, as the former is more accurate, reasonable, efficient, and faster than the latter [11–14] . RSM has been applied in the optimization of analytical procedures, such as the optimization of extraction, compounds separation, and chromatographic parameters [13,14] …”

Section: Introductionmentioning

confidence: 99%

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Optimization of Evaporative Light Scattering Detector using Response Surface Methodology for Liquid Chromatography Analysis of Frondoside A

Tasfiyati¹,

Prasetia²,

Muzdalifah³

et al. 2022

ChemistrySelect

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Response surface methodology is employed to investigate the optimum evaporative light scattering detector (ELSD) operating conditions for high performance liquid chromatography (HPLC) analysis of frondoside A, which is a bioactive compound valuable for its anti-cancer activity. The results suggest that ELSD response is mostly affected by nebulizing gas flow rate and evaporator temperature. The optimum response of frondoside A is achieved by adjusting ELSD evaporator and nebulizer temperature at 80 °C and 35 °C, respectively, with nebulizing gas flow rate of 0.9 SLM. The analysis of variance (ANOVA) test results demonstrated that the established model can successfully describe the relationship between the signal response and ELSD experimental parameters, and predict the optimum ELSD parameters for frondoside A analysis. Furthermore, analytical method validation performed in optimum conditions shows that the proposed method also exhibits adequate sensitivity and linearity, with acceptable precision and accuracy. The results demonstrate the suitability of HPLC-ELSD technique for the development of routine analysis procedures of frondoside A in natural product samples.

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“…Multi-factor experimental designs, such as the response surface methodology (RSM), are widely used for method optimization since they are less laborious and faster as they allow considering mutually influencing experimental factors in a rapid manner. The central composite design (CCD) method, a second-order technique of RSM, has been successfully used to determine the interaction effects among experimental factors and provides closer information of the influencing factors [28,31,32]. Many experimental factors affect the separation efficiency of reversed-phase columns for the tocol isomers.…”

Section: Introductionmentioning

confidence: 99%

Chemometrics-Assisted Optimization of Beta-/Gamma-Tocol Separation on a C30 Stationary Phase in Reversed-Phase LC

Arslan

Janssen

2018

Chromatographia

Self Cite

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This paper presents a chemometrics-assisted optimization study to improve the separation of tocopherol (-T) and tocotrienol (-TT) homologues on a C 30 stationary phase in reversed-phase HPLC. The HPLC settings were optimized using a central composite design and the response surface methodology. Flow rate, column temperature, and mobile phase composition were chosen as independent variables. Peak resolution (R s), analysis time (t R), and peak symmetries of the tocopherol isomers were chosen as response variables. Optimum performance in terms of R s was obtained at a flow rate of 0.31 mL min −1 , a temperature of 8.70 °C, and % B content (methyl tert-butyl ether: methanol: water, 80:18:2, v/v/v) in the mobile phase of 38.12%. The analysis of variance and regression analysis gave adjusted R 2 values of 0.9841 for R s , 0.9850 for t R-(α-T), 0.9853 for t R-(β-T), and 0.9204 for the peak symmetry of β-T. This confirms the good agreement of experimental data with predicted values. The close eluting peaks of β-/γ-tocol could be baseline separated at the optimized conditions at a minimized analysis time. Empirical second-order polynomial models were derived that gave statistically high significances (P < 0.0001). Hence, the models can be successfully employed to predict the optimum separation conditions of co-eluting peaks of β-/γ-tocols. The optimized method was successfully applied to determine the individual tocol homologues in various cold pressed edible oils. Total contents ranged from 15 to almost 2600 mg tocol kg −1 oil.

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Central composite design and response surface methodology for the optimization of Ag+-HPLC/ELSD method for triglyceride profiling

Cited by 11 publications

References 38 publications

Comparative profiling of lactic acid bacteria isolates in optimized and spontaneous fermentation of cowpea leaves

Comparative profiling of lactic acid bacteria isolates in optimized and spontaneous fermentation of cowpea leaves

Optimization of Evaporative Light Scattering Detector using Response Surface Methodology for Liquid Chromatography Analysis of Frondoside A

Chemometrics-Assisted Optimization of Beta-/Gamma-Tocol Separation on a C30 Stationary Phase in Reversed-Phase LC

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