Trichoderma spirale T76-1 displays biocontrol activity against leaf spot on lettuce (Lactuca sativa L.) caused by Corynespora cassiicola or Curvularia aeria

Baiyee, Burhanah; Pornsuriya, Chaninun; S, Itó; Sunpapao, Anurag

doi:10.1016/j.biocontrol.2018.10.018

Cited by 74 publications

(61 citation statements)

References 22 publications

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“…Previous studies have shown that acetaldehyde can inhibit the growth of black mold, Aspergillus niger (Stotzky et al , 1976). Some of the other detected compounds, such as ethanol ( m / z 47.049), acetone ( m / z 59.050) and l‐octen‐3‐ol/3‐octanone (m/z 129.127), were also shown to harbor antifungal activity (Stotzky et al , 1976; Toffano et al , 2017; Baiyee et al , 2019; Lee et al , 2019). Both l‐octen‐3‐ol ( m / z 129.127) and 2‐octenal/l‐octen‐3‐one ( m / z 127.112) were also shown to have other crucial ecological functions, such as regulation of plant and seed growth (Kishimoto et al , 2007; Lee et al , 2019) and attraction of insects (Pierce et al , 1991; Chaiphongpachara et al , 2019).…”

Section: Discussionmentioning

confidence: 99%

Sniffing fungi – phenotyping of volatile chemical diversity inTrichodermaspecies

et al. 2020

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Volatile organic compounds (VOCs) play vital roles in the interaction of fungi with plants and other organisms. A systematic study of the global fungal VOC profiles is still lacking, though it is a prerequisite for elucidating the mechanisms of VOC-mediated interactions. Here we present a versatile system enabling a high-throughput screening of fungal VOCs under controlled temperature. In a proof-of-principle experiment, we characterized the volatile metabolic fingerprints of four Trichoderma spp. over a 48 h growth period.The developed platform allows automated and fast detection of VOCs from up to 14 simultaneously growing fungal cultures in real time. The comprehensive analysis of fungal odors is achieved by employing proton transfer reaction-time of flight-MS and GC-MS. The data-mining strategy based on multivariate data analysis and machine learning allows the volatile metabolic fingerprints to be uncovered.Our data revealed dynamic, development-dependent and extremely species-specific VOC profiles from the biocontrol genus Trichoderma. The two mass spectrometric approaches were highly complementary to each other, together revealing a novel, dynamic view to the fungal VOC release.This analytical system could be used for VOC-based chemotyping of diverse small organisms, or more generally, for any in vivo and in vitro real-time headspace analysis.

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Section: Discussionmentioning

confidence: 99%

Sniffing fungi – phenotyping of volatile chemical diversity inTrichodermaspecies

et al. 2020

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“…T. asperellum has been identified to antagonize the pathogen Fusarium oxysporum , Corynespora cassiicola , and Curvularia aeria . Further, its efficacy has also been demonstrated (Baiyee et al, 2019; Veenstra et al, 2019). B. amyloliquefaciens is a root-colonizing biocontrol bacterium and is employed to battle several phyto-pathogens.…”

Section: Introductionmentioning

confidence: 97%

Co-cultivation of Trichoderma asperellum GDFS1009 and Bacillus amyloliquefaciens 1841 Causes Differential Gene Expression and Improvement in the Wheat Growth and Biocontrol Activity

Karuppiah

Sun

et al. 2019

Front. Microbiol.

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In an effort to balance the demands of plant growth promoting and biological control agents in a single product, the technology on the co-cultivation of two microbes, Trichoderma asperellum GDFS1009 and Bacillus amyloliquefaciens 1841 has been developed and demonstrated its effectiveness in synergistic interactions and its impact on the plant growth and biocontrol potential. In this study, optimization of T. asperellum and B. amyloliquefaciens growth in a single medium was carried out using response surface methodology (RSM). The optimal medium for enhanced growth was estimated as 2% yeast extract, 2% molasses and 2% corn gluten meal. T. asperellum evolved the complicated molecular mechanisms in the co-culture by the induction of BLR-1/BLR-2, VELVET, and NADPH oxidases genes. In performance with these genes, conserved signaling pathways, such as heterotrimeric G proteins and mitogen-activated protein kinases (MAPKs) had also involved in this molecular orchestration. The co-cultivation induced the expression of T. asperellum genes related to secondary metabolism, mycoparasitism, antioxidants and plant growth. On the other hand, the competition during co-cultivation induced the production of new compounds that are not detected in axenic cultures. In addition, the co-culture significantly enhanced the plant growth and protection against Fusarium graminearum . The present study demonstrated the potential of co-cultivation technology could be a used to grow the T. asperellum GDFS1009 and B. amyloliquefaciens 1841 synergistically to improve the production of mycoparasitism related enzymes, secondary metabolites, and plant growth promoting compounds to significantly enhance the plant growth and protection against plant pathogens.

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“…They have been used in the production of enzymes and biological controls of plant diseases (Samuels 1996). Trichoderma species excrete enzymes such as chitinases, β-glucanases and proteases; these enzymes are induced during interactions between Trichoderma and cell walls of phytopathogenic fungi (Wonglom et al 2019;Baiyee et al 2019b). Although other enzymes may be also involved in the complete degradation of cell walls of phytopathogenic fungi, chitinase is generally considered an important enzyme since its substrate, chitin, is the most abundant component in cell walls of many fungus species (Baek et al 1999).…”

Section: Introductionmentioning

confidence: 99%

Characterisation and antifungal activity of extracellular chitinase from a biocontrol fungus, Trichoderma asperellum PQ34

et al. 2019

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Trichoderma species were known as biological control agents against phytopathogenic fungi because they produce a variety of chitinases. Chitinases are hydrolytic enzymes that break down glycosidic bonds in chitin, a major component of the cell walls of fungi. The present study shows that extracellular chitinase activity reached a maximum value of approximately 22 U/mL after 96 h of T. asperellum PQ34 strain culture. The optimal temperature and pH of enzyme are 40°C and 7, respectively, whereas the thermal and pH stability range from 25°C to 50°C and 4 to 10, respectively. Chitinase at 60 U/mL inhibited nearly completely in vitro growth of Colletotrichum sp. (about 95%) and Sclerotium rolfsii (about 97%). In peanut plants, 20 U/mL of chitinase significantly reduced the incidence of S. rolfsii infection compared to controls. The fungal infection incidence of seeds before germination and 30 days after germination was only 2.22% and 2.38%, while the control was 13.33% and 17.95%. Besides, chitinase from T. asperellum PQ34 can also prevent anthracnose that is caused by Colletotrichum sp. on both mango and chilli fruits up to 72 h after enzyme pretreatment at 40 U/mL. In mango and chilli fruits infected with anthracnose, 40 U/mL dose of chitinase inhibited the growth of fungi after 96 h of treatment, the diameter of lesion was only 0.88 cm for mango and 1.45 cm for chilli, while the control was 1.67 cm and 2.85 cm, respectively.

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Trichoderma spirale T76-1 displays biocontrol activity against leaf spot on lettuce (Lactuca sativa L.) caused by Corynespora cassiicola or Curvularia aeria

Cited by 74 publications

References 22 publications

Sniffing fungi – phenotyping of volatile chemical diversity inTrichodermaspecies

Sniffing fungi – phenotyping of volatile chemical diversity inTrichodermaspecies

Co-cultivation of Trichoderma asperellum GDFS1009 and Bacillus amyloliquefaciens 1841 Causes Differential Gene Expression and Improvement in the Wheat Growth and Biocontrol Activity

Characterisation and antifungal activity of extracellular chitinase from a biocontrol fungus, Trichoderma asperellum PQ34

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