Triaging Pap cytology negative, HPV positive cervical cancer screening results with p16/Ki-67 Dual-stained cytology

Petry, K. Ulrich; Schmidt, Dietmar; Scherbring, Sarah; Luyten, Alexander; Reinecke-Lüthge, Axel; Bergeron, Christine; Kommoss, Friedrich; Löning, Thomas; Ordi, Jaume; Regauer, Sigrid; Ridder, Ruediger

doi:10.1016/j.ygyno.2011.02.033

Cited by 193 publications

(174 citation statements)

References 24 publications

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“…10 These studies have consistently demonstrated improved sensitivity of the dual stain for detection of CIN31 compared with cytology as well as increased specificity compared with HPV DNA testing. The p16/Ki-67 dual stain was developed on the premise that it would make the evaluation less subjective and more specific compared with the p16 assay alone, but its reproducibility had not been evaluated previously in a real-life setting.…”

Section: Discussionmentioning

confidence: 91%

“…The p16/Ki-67 or dual-stain assay has been evaluated in several populations. [9][10][11][12] However, a formal reproducibility analysis of the dual-stain assay has not been reported to date. We conducted a systematic reproducibility analysis of 480 p16/Ki-67 dual-stain cytology specimens among 10 raters in a routine US cytology practice compared with an expert evaluation and assessed clinical performance between the evaluators for the detection of cervical intraepithelial neoplasia grade 2 or greater (CIN21).…”

Section: Introductionmentioning

confidence: 99%

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Interobserver reproducibility and accuracy of p16/Ki‐67 dual‐stain cytology in cervical cancer screening

Wentzensen

Fetterman

Tokugawa

et al. 2014

Cancer Cytopathology

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BACKGROUND: Dual-stain cytology for p16 and Ki-67 has been proposed as a biomarker in cervical cancer screening. The authors evaluated the reproducibility and accuracy of dual-stain cytology among 10 newly trained evaluators. METHODS:In total, 480 p16/Ki-67-stained slides from human papillomavirus-positive women were evaluated in masked fashion by 10 evaluators. None of the evaluators had previous experience with p16 or p16/Ki-67 cytology. All participants underwent p16/Ki-67 training and subsequent proficiency testing. Reproducibility of dual-stain cytology was measured using the per-

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Section: Discussionmentioning

confidence: 91%

Section: Introductionmentioning

confidence: 99%

Interobserver reproducibility and accuracy of p16/Ki‐67 dual‐stain cytology in cervical cancer screening

Wentzensen

Fetterman

Tokugawa

et al. 2014

Cancer Cytopathology

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show abstract

“…It is considered to be even better than HPV DNA testing in triage and in the prediction of the clinical outcome of ASC-US and LSILs. [29][30][31][32][33][34][35] The aim of this study was to evaluate the HPV DNA test result, HPV genotype, p16/Ki-67 dual immunostaining and the presence of koilocytosis in LSIL, and their association with its clinical outcome.…”

mentioning

confidence: 99%

HPV16 genotype, p16/Ki‐67 dual staining and koilocytic morphology as potential predictors of the clinical outcome for cervical low‐grade squamous intraepithelial lesions

et al. 2013

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SonjeHPV16 genotype, p16/Ki-67 dual staining and koilocytic morphology as potential predictors of the clinical outcome for cervical low-grade squamous intraepithelial lesions Objective: To evaluate the association of human papillomavirus (HPV) 16 and non-16 genotype, p16/Ki-67 dual staining and koilocytosis and their role in the prediction of the clinical outcome of low-grade squamous intraepithelial lesion (LSIL) cytology. Methods: One hundred and fifty-five patients with LSIL were followed up and recorded as progression, persistence or regression. HPV genotyping was performed for high-risk HPV (hrHPV) DNA-positive cases. Koilocytosis was reviewed and p16/Ki-67 dual staining was performed on reprocessed conventional cytology slides. Results: HPV16 was the most frequent genotype found in 16.3% of cases. p16/Ki-67 dual staining was positive in 36.1% of all cases. Progression, including concurrent cervical intraepithelial lesion grade 2 or above (CIN2+), was recorded in 13.8% of cases. A statistically significant difference between progressive and non-progressive cases was shown by the following: hrHPV-positive versus hrHPV-negative (P = 0.022), HPV16-positive versus non-16 HPV-positive (P < 0.001) and p16/Ki-67-positive versus p16/Ki-67-negative (P < 0.001) cases. Cases with combined HPV16 and p16/Ki-67 positivity showed the highest progression rate (58.3%). Non-koilocytic HPV16-positive cases showed a 50% progression rate compared with 10.1% for koilocytic non-16 HPVpositive cases (P = 0.010). The sensitivity of p16/Ki-67 dual staining for the detection of CIN2+ lesions was 80%, comparable with hrHPV (85%). The specificity of p16/Ki-67 dual staining was 71% and of hrHPV 42%. The highest specificity was found for HPV16 genotype presence (91%), but with low sensitivity (50%). Conclusion: HPV genotyping, p16/Ki-67 dual staining and koilocytic morphology can be useful in the prediction of clinical outcome in women initially diagnosed with LSIL cytology.

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“…In this group, it was too difficult to trust on primary Pap smear samples for absolute distinguishing and diagnosing neoplastic and non-neoplastic lesions, due to high prevalence of cervical atrophy. In the other hand, probability of neoplastic lesion formation in this age group increases significantly (Petry et al, 2011).…”

Section: Discussionmentioning

confidence: 85%

Diagnostic Value of Protein Ki67 (MIB-1) in Atypical Pap Smears of Postmenopausal Women

Fakhrjou

Tabrizi²,

Ghojazadeh³

et al. 2013

Asian Pacific Journal of Cancer Prevention

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Triaging Pap cytology negative, HPV positive cervical cancer screening results with p16/Ki-67 Dual-stained cytology

Cited by 193 publications

References 24 publications

Interobserver reproducibility and accuracy of p16/Ki‐67 dual‐stain cytology in cervical cancer screening

Interobserver reproducibility and accuracy of p16/Ki‐67 dual‐stain cytology in cervical cancer screening

HPV16 genotype, p16/Ki‐67 dual staining and koilocytic morphology as potential predictors of the clinical outcome for cervical low‐grade squamous intraepithelial lesions

Diagnostic Value of Protein Ki67 (MIB-1) in Atypical Pap Smears of Postmenopausal Women

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