An improved reversed-phase (RP) method has been developed for the analysis of triacylglycerols (TAG) in fats and oils. This method has shown superior performance over traditional reversed-phase liquid chromatographic (LC) methods in speed and resolution, and equivalent to superior performance in quantitation accuracy (101% -109%) and reproducibility (1.3% -5.8% RSD). The method can easily be adjusted for use with different oils by simple modification of the gradient and run time. Additionally, a mobile phase is used that is compatible with liquid chromatography/mass spectrometry (LC/MS) that when required, can aid in identification of TAG, for which many standards do not exist. This method is suited to a broad number of applications including (but not limited to) research and development of new oils, quality control, production of industrial or edible oils, and seed strain selection.