Tremella fuciformis Polysaccharides Inhibited Colonic Inflammation in Dextran Sulfate Sodium-Treated Mice via Foxp3+ T Cells, Gut Microbiota, and Bacterial Metabolites

Xu, Yingyin; Xie, Lu; Zhang, Zhiyuan; Zhang, Weiwei; Tang, Jie; He, Xiaolan; Zhou, Jie; Peng, Weihong

doi:10.3389/fimmu.2021.648162

Cited by 47 publications

(48 citation statements)

References 46 publications

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“…Here, DSS treatment also changed the composition of the bacteria community in comparison with the control group; we reviewed the literature and found that it is a common phenomenon. [ 7 , 46 , 47 ] It is understandable because DSS may somehow affect the bacteria community, and on the other hand, colitis is always accompanied by microbiota alteration. [ 48 ] The general enterobacteria composition of each sample at the phylum and family level (Figure 5c,d ) was shown by bar chart and heatmap, respectively.…”

Section: Resultsmentioning

confidence: 99%

Colon‐Targeted Adhesive Hydrogel Microsphere for Regulation of Gut Immunity and Flora

et al. 2021

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Intestinal immune homeostasis and microbiome structure play a critical role in the pathogenesis and progress of inflammatory bowel disease (IBD), whereas IBD treatment remains a challenge as the first-line drugs show limited therapeutic efficiency and great side effect. In this study, a colon-targeted adhesive core-shell hydrogel microsphere is designed and fabricated by the ingenious combination of advanced gas-shearing technology and ionic diffusion method, which can congregate on colon tissue regulating the gut immune-microbiota microenvironment in IBD treatment. The degradation experiment indicates the anti-acid and colon-targeted property of the alginate hydrogel shell, and the in vivo imaging shows the mucoadhesive ability of the thiolated-hyaluronic acid hydrogel core of the microsphere, which reduces the systematic exposure and prolongs the local drug dwell time. In addition, both in vitro and in vivo study demonstrate that the microsphere significantly reduces the secretion of pro-inflammatory cytokines, induces specific type 2 macrophage differentiation, and remarkably alleviates colitis in the mice model. Moreover, 16S ribosomal RNA sequencing reveals an optimized gut flora composition, probiotics including Bifidobacterium and Lactobacillus significantly augment, while the detrimental communities are inhibited, which benefits the intestinal homeostasis. This finding provides an ideal clinical candidate for IBD treatment.

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Section: Resultsmentioning

confidence: 99%

Colon‐Targeted Adhesive Hydrogel Microsphere for Regulation of Gut Immunity and Flora

et al. 2021

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“…Interestingly, some investigators revealed that the amount of intestinal beneficial bacteria is associated with the immune function of the host [ 32 ]. For instance, Xu et al (2021) [ 33 ] found that the increased concentrations of serum IgG and IgA were positively correlated with the increased abundance of intestinal Lactobacillus . Furthermore, the by-products produced from bifidobacterial carbohydrate metabolism act as vectors that directly and indirectly trigger the mucosa-associated immune cells to enhance the immune system of the host [ 34 , 35 ].…”

Section: Discussionmentioning

confidence: 99%

Influence of Sodium Humate on the Growth Performance, Diarrhea Incidence, Blood Parameters, and Fecal Microflora of Pre-Weaned Dairy Calves

Wang

You

et al. 2022

Animals

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This study aimed to evaluate the effects of the administration of sodium humate (NaH) on the growth performance, diarrhea incidence, and fecal microflora of pre-weaned Holstein calves. In a 53-day experiment, forty healthy newborn female calves were randomly allocated to the following four treatment groups: (1) control (basal diet); (2) 1-gram NaH (basal diet extra orally supplemented with 1 g of NaH dissolved in 100 mL of milk or milk replacer daily); (3) 3-gram NaH (basal diet extra orally supplemented with 3 g of NaH dissolved in 100 mL of milk or milk replacer daily); and (4) 5-gram NaH (basal diet extra orally supplemented with 5 g of NaH dissolved in 100 mL of milk or milk replacer daily). NaH was mixed with milk (d 2–20) or milk replacer (d 21–53). Calves in the 5-gram NaH group had a higher ADG during d 1 to 21 and d 21 to 53 than the other groups did (p < 0.05). Fecal scores and diarrheal incidence were significantly lower in the 3-gram and 5-gram NaH groups than the 1-gram NaH and control groups during d 1 to 20 (p < 0.05). The serum IgA, IgG and IL-4 concentrations, and T-SOD and T-AOC activities were higher, and the serum IL-6, TNF-α, D-lactic acid, and MDA concentrations were lower in the 5-gram NaH group than the control group (p < 0.05). Furthermore, NaH supplementation increased the abundances of Bifidobacterium and Lactobacillus but decreased the abundance of Escherichia coli in feces (p < 0.05). These encouraging findings indicated that supplementation with 5 g of NaH effectively improved the immune status, antioxidant capacity, and intestinal beneficial bacteria, and further improved the growth performance and reduced the diarrhea incidence of the pre-weaned dairy calves.

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“…The column was then eluted with distilled water, 50 mM, 150 mM, and 1 M NaCl successively at flow rates of 10 ml/min. After assessment of their hepatoprotective effects, the 50 mM NaCl eluted part was gathered and lyophilized as FVPs for further research ( Xu et al, 2021b ).…”

Section: Methodsmentioning

confidence: 99%

“…The weight-average (Mw) and number-average (Mn) molecular weights of the FVPs were determined via high-performance gel permeation chromatography, as previously described in the literature ( Xu et al, 2021a ). Fourier transform infrared (FT-IR) spectra of the FVPs were captured using an FT-IR microscope (model iN 10, Thermo Nicolet Corp.; Madison, WI, United States) fitted with a liquid nitrogen-cooled mercury-cadmium-telluride detector ( Xu et al, 2021b ).…”

Section: Methodsmentioning

confidence: 99%

Flammulinavelutipes Polysaccharides Modulate Gut Microbiota and Alleviate Carbon Tetrachloride-Induced Hepatic Oxidative Injury in Mice

Zhang

Wang

et al. 2022

Front. Microbiol.

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A carbon tetrachloride-induced acute liver injury mouse model is used to study the regulation of gut microbiota and hepatoprotective effect of polysaccharides from Flammulina velutipes (FVPs). The hepatoprotective effect of the FVPs leads to reduced levels of serum aspartate transaminase (AST), alanine aminotransferase (ALT), triglyceride (TG), total cholesterol (TC), total bile acid (TBA) content, and change in liver histopathology. Their anti-oxidant activity is exhibited by decreased levels of hepatic malonaldehyde (MDA) and protein carbonyl (PC) content and increased catalase (CAT) and superoxide dismutase (SOD) content. The anti-inflammatory ability of the FVPs is reflected in a decrease in pro-inflammatory cytokines (including IL-6, IL-1β, and TNF-α). 16S rRNA sequencing shows that the FVPs change the composition of the gut microbiota. A subsequent metabolomics analysis of the gut bacteria (UHPLC–MS/MS-based) revealed that fatty acid biosynthesis, tryptophan metabolism, and metabolism of xenobiotics by cytochrome P450 play important roles in the hepatoprotective effect. This study provides a potential way to modulate gut microbiota and manage liver diseases using natural products.

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Tremella fuciformis Polysaccharides Inhibited Colonic Inflammation in Dextran Sulfate Sodium-Treated Mice via Foxp3+ T Cells, Gut Microbiota, and Bacterial Metabolites

Cited by 47 publications

References 46 publications

Colon‐Targeted Adhesive Hydrogel Microsphere for Regulation of Gut Immunity and Flora

Colon‐Targeted Adhesive Hydrogel Microsphere for Regulation of Gut Immunity and Flora

Influence of Sodium Humate on the Growth Performance, Diarrhea Incidence, Blood Parameters, and Fecal Microflora of Pre-Weaned Dairy Calves

Flammulinavelutipes Polysaccharides Modulate Gut Microbiota and Alleviate Carbon Tetrachloride-Induced Hepatic Oxidative Injury in Mice

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