Treatment of Wound Healing Disorders of Radial Forearm Free Flap Donor Sites Using Cold Atmospheric Plasma: A Proof of Concept

Härtwig, Sven; Doll, Christian; Voß, Jan Oliver; Hertel, Moritz; Preissner, Saskia; Raguse, Jan Dirk

doi:10.1016/j.joms.2016.08.011

Cited by 49 publications

(30 citation statements)

References 28 publications

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“…with regard to scar formation or prevention of complications in wound healing. Possible fields of application of CAP in acute wound healing could be in patients with co-morbidities leading to a high risk of disturbed wound healing and subsequent chronification, in the case of large-area burns or in the treatment of skin graft donor and acceptor sites (92,94).…”

Section: Biological Plasma Effects and Its Medical Use: Focus On Wounmentioning

confidence: 99%

Plasma Medicine: A Field of Applied Redox Biology

Schmidt

Bekeschus

et al. 2019

In Vivo

221

153

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Section: Biological Plasma Effects and Its Medical Use: Focus On Wounmentioning

confidence: 99%

Plasma Medicine: A Field of Applied Redox Biology

Schmidt

Bekeschus

et al. 2019

In Vivo

221

153

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“…For that reason, alternative wound healing therapies have been searched and extended. Very recently, special attention has been paid to the use of cold atmospheric pressure plasma (CAPP) in dermatology, thanks to the ability of these ionized-gas sources is to improve the wound healing process [5][6][7][8][9][10][11][12][13][14][15][16][17][18].…”

Section: Introductionmentioning

confidence: 99%

“…Over the past few years, the interest of the controlled applications of CAPPs has rapidly increased. They include not only the utilization of CAPPs in dermatology [5][6][7][8][9][10][11][12][13][14][15][16][17][18], but also application of CAPP in the synthesis of nanomaterials [19][20][21], the germination of seeds [22], inhibiting the proliferation of cancers cell lines [23,24], and so on. This is possible because CAPP operation results in the generation of different reactive oxygen species (ROS, i.e., O 3 , H 2 O 2 , O•, O − 2 , OH•) and reactive nitrogen species (RNS, i.e., NO, NO 2 ), in addition to the emission of UV radiation and heat [7-9, 18, 25, 26].…”

Section: Introductionmentioning

confidence: 99%

“…To date, a few reaction-discharge systems have been developed for further applications comprise medicine and dermatology. These reaction-discharge systems include dielectric barrier discharges (DBDs) [14,15], corona discharges (CDs) [37], microwave-induced plasmas (MIPs) [11,12], and plasma jets named kINPen [5,[7][8][9][10]17] or COST [38,39], in addition to the non-thermal atmospheric pressure plasma jets studied and utilized by e.g. Rad et al [40], Gidon et al [41,42], Darmawati et al [43], Cheng et al [44], Busco et al [45].…”

Section: Introductionmentioning

confidence: 99%

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Activation of the Normal Human Skin Cells by a Portable Dielectric Barrier Discharge-Based Reaction-Discharge System of a Defined Gas Temperature

Dzimitrowicz

Bielawska‐Pohl

Jamróz

et al. 2019

Plasma Chem Plasma Process

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Skin injury leading to chronic wounds is of high interest due to the increasing number of patients suffering from this symptom. Proliferation, migration, and angiogenesis are key factors in the wound healing processes. For that reason, controlled promotion of these processes is required. In this work, we present the portable helium-dielectric barrier discharge (He-DBD)-based reaction-discharge system of controlled gas temperature for biological activities. To make this He-DBD-based reaction-discharge system safe for biological purposes, a multivariate optimization of the operating parameters was performed. To evaluate the effect of the He-DBD operating parameters on the rotational gas temperature T rot (OH), a design of experiment followed by a Response Surface Methodology was applied. Based on the suggested statistical model, the optimal operating conditions under which the T rot (OH) is less than 37 °C (310 K) were estimated. Then, the resulted model was validated in order to confirm its accuracy. After estimation the optical operating conditions of He-DBD operation, the spectroscopic characteristic of the He-DBD-based reaction-discharge system in relevance to the several optical temperatures in addition to electron number density has been carried out. Additionally, the qualitative and quantitative analyses of the reactive oxygen species and reactive nitrogen species were performed in order to investigate of reactions and processes running in the He-DBD-gaseous phase and in the He-DBD-treated liquid. Next, the developed portable He-DBD-based reaction-discharge system, working under the optimal operating conditions, was used to stimulate the wound healing process. It was found that a 30 s He-DBD treatment significantly increased the proliferation, migration, and angiogenesis of keratinocytes (HaCaT) and fibroblasts (MSU-1.1) cell lines, as well as human skin microvascular endothelial cells (HSkMEC.2). Hence, the application of the cold atmospheric pressure plasma generated in this He-DBD-based reaction-discharge system might be an alternative therapy for patient suffering from chronic wounds. Keywords Wound healing • Angiogenesis • Migration • Proliferation • Cold atmospheric pressure plasma Abbreviations BBD Box-Behnken design Anna Dzimitrowicz and Aleksandra Bielawska-Pohl have equally contributed to this work.

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“…Kako u ovim studijama oštećenja na koži nisu bila inficirana mikroorganizmima, rezultati istraživanja ukazuju na potencijal plazme da stimulira regeneraciju tkiva neovisno o antiseptičkim učincima CAP-a 5 . I noviji izvještaji o kliničkim studijama provedenim na ljudima također govore u prilog CAP-u kao novom pristupu tretiranja kroničnih rana 36,37 . Iako se mehanizmi djelovanja CAP-a na rane još uvijek istražuju, trenutno je prihvaćen koncept plazmom potpomognutog zacjeljivanja kožnih rana u kojem CAP djeluje u dvije nezavisne faze: 1) antiseptičko djelovanje plazme inaktivacijom bakterija bez oštećivanja okolnog zdravog tkiva; i 2) stimulacija regeneracije tkiva i procesa zacjeljivanja 7 .…”

Section: Dermatologijaunclassified

Plasma applications in medicine and dentistry

Mance

2017

Med. flum.

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Sažetak. U fizici, plazma je ioniziran, kvazineutralan plin za koji se često kaže da je četvrto stanje materije. Dugo vremena se plazma u medicini koristila zbog svojih termalnih svojstava, primjerice za sterilizaciju kirurškog i medicinskog pribora te za rezanje i kauterizaciju tkiva. Generiranjem hladne plazme u atmosferskim uvjetima započelo je novo razdoblje u primjeni plazme u biomedicini. Plazma medicina novo je interdisciplinarno znanstveno područje koje kombinira spoznaje fizike plazme s biomedicinskim i kliničkim znanostima. Hladna atmosferska plazma emitira elektromagnetno zračenje te se sastoji od mnoštva nabijenih i nenabijenih čestica, među kojima su najznačajnije reaktivne kisikove i dušikove vrste. Smatra se da su upravo reaktivne kisikove vrste najvažnije za biološke učinke plazme kao što su inaktivacija mikroorganizama i biofilmova. U dermatologiji se pokazalo da hladna atmosferska plazma pozitivno utječe na zacjeljivanje kroničnih rana. Prvi instrumenti za primjenu hladne plazme na ljudima već su i komercijalno dostupni. Primjena hladne plazme ima velik potencijal za primjenu u onkologiji, ali i u stomatologiji. Mnogobrojne studije dale su vrlo obećavajuće rezultate za primjenu hladne plazme u medicini, no treba imati na umu da mehanizmi međudjelovanja plazme s biološkim tkivom još uvijek nisu u potpunosti razjašnjeni, da nema dogovorom prihvaćene definicije doze koju plazma predaje materiji, da još uvijek nema standardiziranih instrumenata za generiranje i karakterizaciju plazme te da za pravu procjenu potencijala primjene atmosferske plazme u medicini i stomatologiji nedostaje više detaljnih in vivo studija.Ključne riječi: dermatologija; onkologija; plazma; stomatologija Abstract. Physical plasma is an ionized, quasi-neutral gas. It is often said to be the fourth state of matter. For a long time, plasma was used in medicine exclusively because of its thermal properties for sterilization purposes of surgical equipment and medical supplies, and also to cut and cauterize tissue. The possibility to generate cold plasma in atmospheric conditions marked the beginning of a completely new era in the application of plasma in biomedicine. Plasma medicine is a new interdisciplinary field of science that combines the knowledge of plasma physics to biomedical and clinical sciences. Cold atmospheric plasma emits electromagnetic radiation and it consists out of charged and uncharged particles, among those reactive oxygen and nitrogen species are the most important ones. It is considered that reactive oxygen species cause the most important biological plasma effects, such as inactivation of microorganisms and biofilms. It has been shown in dermatology that cold atmospheric plasma has a healing effect on chronic wounds. First instruments for the application of cold plasma in humans are already commercially available. Cold plasma has a great potential for application in oncology and in dentistry. Numerous studies have yielded very promising results for the application of cold plasma in medicine, but it should ...

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Treatment of Wound Healing Disorders of Radial Forearm Free Flap Donor Sites Using Cold Atmospheric Plasma: A Proof of Concept

Cited by 49 publications

References 28 publications

Plasma Medicine: A Field of Applied Redox Biology

Plasma Medicine: A Field of Applied Redox Biology

Activation of the Normal Human Skin Cells by a Portable Dielectric Barrier Discharge-Based Reaction-Discharge System of a Defined Gas Temperature

Plasma applications in medicine and dentistry

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