Plasma Medicine: A Field of Applied Redox Biology

T, Von Woedtke; Schmidt, Anke; Bekeschus, Sander; Wende, Kristian; Weltmann, K-D

doi:10.21873/invivo.11570

Cited by 198 publications

(126 citation statements)

References 177 publications

(254 reference statements)

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“…[ 5 ] The current indication of medical plasma therapy is to promote beneficial effects on the healing of chronic wounds and ulcers, apart from other dermatological indications. [ 6 ] Strikingly, antitumor efficacy in head and neck cancer patients suggested plasma treatment to have a role in oncology as well. [ 7 ] The concept linking these seemingly unrelated effects of stimulation of healing in non‐healing wounds on the one hand, and killing of tumor cells on the other hand is termed hormesis.…”

Section: Introductionmentioning

confidence: 99%

Medical Gas Plasma Jet Technology Targets Murine Melanoma in an Immunogenic Fashion

et al. 2020

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Medical technologies from physics are imperative in the diagnosis and therapy of many types of diseases. In 2013, a novel cold physical plasma treatment concept was accredited for clinical therapy. This gas plasma jet technology generates large amounts of different reactive oxygen and nitrogen species (ROS). Using a melanoma model, gas plasma technology is tested as a novel anticancer agent. Plasma technology derived ROS diminish tumor growth in vitro and in vivo. Varying the feed gas mixture modifies the composition of ROS. Conditions rich in atomic oxygen correlate with killing activity and elevate intratumoral immune‐infiltrates of CD8+ cytotoxic T‐cells and dendritic cells. T‐cells from secondary lymphoid organs of these mice stimulated with B16 melanoma cells ex vivo show higher activation levels as well. This correlates with immunogenic cancer cell death and higher calreticulin and heat‐shock protein 90 expressions induced by gas plasma treatment in melanoma cells. To test the immunogenicity of gas plasma treated melanoma cells, 50% of mice vaccinated with these cells are protected from tumor growth compared to 1/6 and 5/6 mice negative control (mitomycin C) and positive control (mitoxantrone), respectively. Gas plasma jet technology is concluded to provide immunoprotection against malignant melanoma both in vitro and in vivo.

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Section: Introductionmentioning

confidence: 99%

Medical Gas Plasma Jet Technology Targets Murine Melanoma in an Immunogenic Fashion

et al. 2020

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“…Cold Atmospheric Plasma (CAP) is an emerging tool of biomedical and clinical importance that controls cellular processes such as wound healing, immunomodulation, cell death, and cancer. CAP is a partially ionized molecular or noble gas or gas mixture that generates a plethora of reactive oxygen and nitrogen species (ROS/RNS), radiation (ultraviolet (UV), visible, and infrared), and small chemical entities such as neutral molecules, ions, electrons, and exited atoms under atmospheric pressure and ambient temperature [1].…”

Section: Introductionmentioning

confidence: 99%

“…Recent reports also indicate that CAP supports wound healing by influencing cell viability [7,8], proliferation [9], migration [10], inflammation [11], dermal regeneration, and reepithelization [12]. Currently, only a small number of studies have examined the cellular and molecular effects of CAP-mediated wound healing [1,13,14] and the mechanisms underlying CAP-mediated wound healing in the context of regenerative pathways require further investigation.…”

Section: Introductionmentioning

confidence: 99%

The HIPPO Transducer YAP and Its Targets CTGF and Cyr61 Drive a Paracrine Signalling in Cold Atmospheric Plasma-Mediated Wound Healing

Shome

Woedtke

Riedel

et al. 2020

Oxidative Medicine and Cellular Longevity

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Reactive species play a pivotal role in orchestrating wound healing responses. They act as secondary messengers and drive redox-signalling pathways that are involved in the homeostatic, inflammatory, proliferative, and remodelling phases of wound healing. The application of Cold Atmospheric Plasma (CAP) to the wound site produces a profusion of short-and long-lived reactive species that have been demonstrated to be effective in promoting wound healing; however, knowledge of the mechanisms underlying CAP-mediated wound healing remains scarce. To address this, an in vitro coculture model was used to study the effects of CAP on wound healing and on paracrine crosstalk between dermal keratinocytes and fibroblasts. Using this coculture model, we observed a stimulatory effect on the migration ability of HaCaT cells that were cocultured with dermal fibroblasts. Additionally, CAP treatment resulted in an upregulation of the HIPPO transcription factor YAP in HaCaTs and fibroblasts. Downstream effectors of the HIPPO signalling pathway (CTGF and Cyr61) were also upregulated in dermal fibroblasts, and the administration of antioxidants could inhibit CAP-mediated wound healing and abrogate the gene expression of the HIPPO downstream effectors. Interestingly, we observed that HaCaT cells exhibited an improved cell migration rate when incubated with CAP-treated fibroblast-conditioned media compared to that observed after incubation with untreated media. An induction of CTGF and Cyr61 secretion was also observed upon CAP treatment in the fibroblast-conditioned media. Finally, exposure to recombinant CTGF and Cyr61 could also significantly improve HaCaT cell migration. In summary, our results validated that CAP activates a regenerative signalling pathway at the onset of wound healing. Additionally, CAP also stimulated a reciprocal communication between dermal fibroblasts and keratinocytes, resulting in improved keratinocyte wound healing in coculture.

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“…One such innovative procedure, which complements the intraoperative treatment of tumor tissue, could be CAP treatment of the resection margins. The antitumor effects of CAP have already been demonstrated in some cancer entities such as melanoma and pancreatic and breast carcinomas [24][25][26]. Many of the cellular effects, such as reactive oxygen species (ROS) and reactive nitrogen species (RNS) response as products of CAP treatment, have been already described in detail [27].…”

Section: Discussionmentioning

confidence: 99%

“…The antitumor effects of CAP have already been demonstrated in some cancer entities such as melanoma and pancreatic and breast carcinomas [23][24][25]. Many of the cellular effects, such as reactive oxygen species (ROS) and reactive nitrogen species (RNS) response as products of CAP treatment, have been already described in detail [26]. Possible events after CAP treatment triggered by intracellular reactive species, including DNA damage [27], lipid peroxidation [28], or mitochondrial dysfunction [29,30], have also been reported.…”

Section: Discussionmentioning

confidence: 99%

Cold Atmospheric Plasma Treatment of Chondrosarcoma Cells Affects Proliferation and Cell Membrane Permeability

Haralambiev

Nitsch

Jacoby

et al. 2020

IJMS

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Chondrosarcoma is the second most common malign bone tumor in adults. Surgical resection of the tumor is recommended because of its resistance to clinical treatment such as chemotherapy and radiation therapy. Thus, the prognosis for patients mainly depends on sufficient surgical resection. Due to this, research on alternative therapies is needed. Cold atmospheric plasma (CAP) is an ionized gas that contains various reactive species. Previous studies have shown an anti-oncogenic potential of CAP on different cancer cell types. The current study examined the effects of treatment with CAP on two chondrosarcoma cell lines (CAL-78, SW1353). Through proliferation assay, the cell growth after CAP-treatment was determined. A strong antiproliferative effect for both cell lines was detected. By fluorescein diacetate (FDA) assay and ATP release assay, alterations in the cell membrane and associated translocation of low molecular weight particles through the cytoplasmic membrane were observed. In supernatant, the non-membrane-permeable FDA and endogenously synthesized ATP detected suggest an increased membrane permeability after CAP treatment. Similar results were shown by the dextran-uptake assay. Furthermore, fluorescence microscopic G-/F-actin assay was performed. G- and F-actin were selectively dyed, and the ratio was measured. The presented results indicate CAP-induced changes in cell membrane function and possible alterations in actin-cytoskeleton, which may contribute to the antiproliferative effects of CAP.

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Plasma Medicine: A Field of Applied Redox Biology

Cited by 198 publications

References 177 publications

Medical Gas Plasma Jet Technology Targets Murine Melanoma in an Immunogenic Fashion

Medical Gas Plasma Jet Technology Targets Murine Melanoma in an Immunogenic Fashion

The HIPPO Transducer YAP and Its Targets CTGF and Cyr61 Drive a Paracrine Signalling in Cold Atmospheric Plasma-Mediated Wound Healing

Cold Atmospheric Plasma Treatment of Chondrosarcoma Cells Affects Proliferation and Cell Membrane Permeability

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