Activation of the Normal Human Skin Cells by a Portable Dielectric Barrier Discharge-Based Reaction-Discharge System of a Defined Gas Temperature

Abstract

Skin injury leading to chronic wounds is of high interest due to the increasing number of patients suffering from this symptom. Proliferation, migration, and angiogenesis are key factors in the wound healing processes. For that reason, controlled promotion of these processes is required. In this work, we present the portable helium-dielectric barrier discharge (He-DBD)-based reaction-discharge system of controlled gas temperature for biological activities. To make this He-DBD-based reaction-discharge system safe for biological purposes, a multivariate optimization of the operating parameters was performed. To evaluate the effect of the He-DBD operating parameters on the rotational gas temperature T rot (OH), a design of experiment followed by a Response Surface Methodology was applied. Based on the suggested statistical model, the optimal operating conditions under which the T rot (OH) is less than 37 °C (310 K) were estimated. Then, the resulted model was validated in order to confirm its accuracy. After estimation the optical operating conditions of He-DBD operation, the spectroscopic characteristic of the He-DBD-based reaction-discharge system in relevance to the several optical temperatures in addition to electron number density has been carried out. Additionally, the qualitative and quantitative analyses of the reactive oxygen species and reactive nitrogen species were performed in order to investigate of reactions and processes running in the He-DBD-gaseous phase and in the He-DBD-treated liquid. Next, the developed portable He-DBD-based reaction-discharge system, working under the optimal operating conditions, was used to stimulate the wound healing process. It was found that a 30 s He-DBD treatment significantly increased the proliferation, migration, and angiogenesis of keratinocytes (HaCaT) and fibroblasts (MSU-1.1) cell lines, as well as human skin microvascular endothelial cells (HSkMEC.2). Hence, the application of the cold atmospheric pressure plasma generated in this He-DBD-based reaction-discharge system might be an alternative therapy for patient suffering from chronic wounds. Keywords Wound healing • Angiogenesis • Migration • Proliferation • Cold atmospheric pressure plasma Abbreviations BBD Box-Behnken design Anna Dzimitrowicz and Aleksandra Bielawska-Pohl have equally contributed to this work.

Combined Toxicity of Gas Plasma Treatment and Nanoparticles Exposure in Melanoma Cells In Vitro

“…Studies using both gas plasma exposure and cerium oxide nanoparticles have not been reported yet. A range of studies are available for silver nanoparticles, demonstrating combined cytotoxicity with gas plasma treatment in Hs 294T human melanoma cells [ 57 ] and even antimicrobial efficacy in bacterial phytopathogens [ 58 ]. Gas plasma exposure in glioblastoma cells also led to enhanced silver nanoparticle uptake rates and enhanced cytotoxicity [ 59 ].…”

The Influence of Cold Atmospheric Pressure Plasma-Treated Media on the Cell Viability, Motility, and Induction of Apoptosis in Human Non-Metastatic (MCF7) and Metastatic (MDA-MB-231) Breast Cancer Cell Lines

“…To obtain the CAPP-activated media, including the DMEM and the Opti-MEM, a CAPP-based reaction-discharge system, previously developed and optimized in our research group, was used [ 33 ]. In this CAPP-based reaction-discharge system, a DBD plasma jet is sustained in He and used as a CAPP source.…”

“…The optimal operating conditions of this reaction-discharge system, leading to the CAPP-activated media production, were as follows: The frequency of the modulation: 2.14 kHz; the duty cycle: 74.29%; and the He flow rate 10.6 L min −1 . Under these optimal operating conditions, the gas temperature (based on the OH (0-0) radical emission spectrum) was about 37 °C (310 K) [ 33 ]. The additional electrical parameters, i.e., the voltage amplitude, the voltage waveform shape, and the general frequency, were assessed using a digital two-channel storage oscilloscope (Tektronix, TBS 1000, Beaverton, OR, USA) and were as follows: (i) the voltage amplitude: 6 kV; (ii) the voltage waveform: square wave; and (iii) the general frequency: 66.45 kHz.…”

“…In this study, we examined the influence of two CAPP-treated media, including DMEM and Opti-MEM, on the biological activity of three cell lines: The highly metastatic human breast cancer MDA-MB-231 cell line, the non-invasive human breast cancer MCF7 cell line, and the non-cancerous human normal MCF10A cell line. To reach this aim, we developed and used a special portable CAPP-based reaction-discharge system, which had already been successfully applied for the activation of normal human skin cell lines [ 33 ]. To the best of our knowledge, this was the first mobile and inexpensive in-operation CAPP-based reaction-discharge system in which a DBD plasma jet was applied for human cell line treatment.…”

Implementation of a Non-Thermal Atmospheric Pressure Plasma for Eradication of Plant Pathogens from a Surface of Economically Important Seeds

“…Therefore, the discharge parameters of the applied NTAPP, such as the amplitude of voltage, the duty cycle, and the flow rate of the discharge gas, should have been fully optimized prior to use. Having this in mind, we focused on the DBD-generating portable device that we previously constructed and studied for medical purposes [29]. As NTAPP remains the source of an electromagnetic field, heat radiation as well as UV photons, we investigated whether the DBD exposure causes a physical damage to the plant material or alters the germination-associated properties of mung bean seeds.…”