Transposition of DNA fragments flanked by two inverted Tn1 sequences: translocation of the plasmid RP4::Tn1 region harboring the Tcr marker

Dobritsa, Anatoly P.; Ivanova, Zinaida A.; Fedoseeva, V. B.

doi:10.1016/0378-1119(83)90108-7

Cited by 4 publications

(2 citation statements)

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“…A potentially significant consequence of a plasmid carrying two copies of T n l is the transposition of the two copies of Tnl together with the flanked DNA (Dobritsa et al, 1983). Plasmid pUB2946 is the consequence of just such a transposition from pUB2660 to R388.…”

Section: Discussionmentioning

confidence: 99%

“…Hence the potential for redistribution of normally non-transposable genes, as incorporated in Tn826, would seem to be considerable. The arrangement of DNA comprising Tn826 is one of two possible transposons incorporating pUB2660 DNA in addition to Tnl (Dobritsa et al, 1983). This transposition of normally nontransposable DNA overcomes the host-range limitation of pUB2660, exemplified by its inability to transfer from P .…”

Section: Discussionmentioning

confidence: 99%

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Evolution of an R Plasmid from a Cryptic Plasmid by Transposition of Two Copies of Tn1 in Providencia stuartii

Hawkey¹,

Bennett²,

Hawkey³

1985

Microbiology

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Examination of a series of isolates of Providencia stuartii collected over an 18 month period from a chronic-care patient at Bristol Royal Infirmary revealed the emergence of resistance to carbenicillin. Resistance was mediated by a 47 kb plasmid which transferred by conjugation to a plasmid-free strain of P. stuartii but not to Escherichia coli. Carbenicillin-sensitive isolates were either plasmid-free or contained a 36 kb cryptic plasmid. Restriction endonuclease mapping of this plasmid showed it to be closely related to 32 kb and 34 kb cryptic plasmids reported previously in P. stuartii from Bristol. Mapping of the R plasmid showed it to be derived from the 34 kb cryptic plasmid by transposition of two copies of Tn1.

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Section: Discussionmentioning

confidence: 99%

Section: Discussionmentioning

confidence: 99%

Evolution of an R Plasmid from a Cryptic Plasmid by Transposition of Two Copies of Tn1 in Providencia stuartii

Hawkey¹,

Bennett²,

Hawkey³

1985

Microbiology

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IS8- and Tn2353-mediated cointegration of the plasmids R15 and RP4:: Tn1

Dobritsa¹

1984

Molec. Gen. Genet.

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The plasmids R15 and RP4::Tn1 form fused structures (85 Md and 92 Md cointegrates). The cointegrates do not resolve practically in recA- Escherichia coli cells and have a mean life-time of more than 50 generations in a recA+ background. The 85 Md cointegrates were generated at a frequency of 4 x 10(-4) per R15 transconjugant during a mating between E. coli [R15; RP4::Tn1] and E. coli [F' ColVBtrp :: Tn1755 ]. These plasmids carry two directly repeated copies of the mobile element IS8 at the junctions between R15 and RP4::Tn1. The transposition of IS8 from RP4::Tn1 to the R15 plasmid and the formation of hybrid molecules promoted by this process appear to be induced by the IS8 element of the Tn1755 structure during or after conjugal transfer of F' ColVBtrp :: Tn1755 into E. coli [R15; RP4::Tn1] cells. The formation of the 92 Md cointegrates occurs at a frequency of 2 x 10(-5). The fused molecules of R15 and RP4::Tn1 carry two direct copies of an 8.65 Md R15 fragment at the junctions between these replicons. The fragment has specific features of a new transposon. This element designated Tn2353 determines resistance to Hg, Sm and Su and contains two sites for each BamHI, Bg/II and Sa/I and three sites for both EcoRI and PstI. The physical map and some other characteristics of Tn2353 are presented.

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