Evolution of an R Plasmid from a Cryptic Plasmid by Transposition of Two Copies of Tn1 in Providencia stuartii

Hawkey, Peter M.; Bennett, Peter M.; Hawkey, Carol A.

doi:10.1099/00221287-131-4-927

Cited by 9 publications

(5 citation statements)

References 19 publications

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“…Nevertheless, the formation of new transposon structures is almost never observed directly in clinical or environmental settings, presumably because of the low frequency with which the events occur. The exceptions to this include the observation by Hawkey et al (19) of the probable path of evolution of an R plasmid from a cryptic plasmid in clinical isolates of Providencia stuartii collected over an 18-month period from a single chronic-care patient in the Bristol Royal Infirmary. van der Ploeg et al (49) observed transposition of the class I insertion sequence IS1247 from an unlinked site on the chromosome of Xanthobacter autotrophicus GJ10 to a chromosomal location upstream of the haloacetate dehalogenase gene dhlB.…”

Section: Fig 3 Junction R Dna Sequences Of Pcpe3 and Tn5271mentioning

confidence: 99%

Structures of Homologous Composite Transposons Carrying cbaABC Genes from Europe and North America

Gioia

Peel

Fava

et al. 1998

Appl Environ Microbiol

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IS1071 is a class II transposable element carrying atnpA gene related to the transposase genes of the Tn3 family. Copies of IS1071 that are conserved with more than 99% nucleotide sequence identity have been found as direct repeats flanking a remarkable variety of catabolic gene sequences worldwide. The sequences of chlorobenzoate catabolic transposons found on pBRC60 (Tn5271) in Niagara Falls, N.Y., and on pCPE3 in Bologna, Italy, show that these transposons were formed from highly homologous IS1071 and cbaABCcomponents (levels of identity, >99.5 and >99.3%, respectively). Nevertheless, the junction sequences between the IS1071Land IS1071R elements and the internal DNA differ by 41 and 927 bp, respectively, suggesting that these transposons were assembled independently on the two plasmids. The formation of the right junction in both transposons truncated an open reading frame for a putative aryl-coenzyme A ligase with sequence similarity to benzoate- andp-hydroxybenzoate-coenzyme A ligases ofRhodopseudomonas palustris.

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Section: Fig 3 Junction R Dna Sequences Of Pcpe3 and Tn5271mentioning

confidence: 99%

Structures of Homologous Composite Transposons Carrying cbaABC Genes from Europe and North America

Gioia

Peel

Fava

et al. 1998

Appl Environ Microbiol

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“…The laboratory numbers used and strain sources are indicated in Table 1. Further details on three (A627/85, A628/ 85, and A629/85) strains, which were received from P. Hawkey, University of Leeds, Leeds, United Kingdom, have been published previously (11,12). The majority of strains (17 of 26) were isolated from urine specimens, and .0 a All strains were urine isolates except for those marked (w), which were wound swab isolates, or (o), which were isolated from other material (blood or unspecified source).…”

Section: Methodsmentioning

confidence: 99%

Detection of genomic variation in Providencia stuartii clinical isolates by analysis of DNA restriction fragment length polymorphisms containing rRNA cistrons

Owen¹,

Beck²,

Dayal³

et al. 1988

J Clin Microbiol

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Chromosomal DNA from 26 strains of Providencia stuartii isolated mainly in hospitals in the United Kingdom and reference strains of P. stuartii, P. rustigianii, and Proteus vulgaris were digested with the restriction endonucleases EcoRI and HindIII. After electrophoresis in agarose gels, the fragments were subjected to Southern blot hybridization analysis with a biotin-labeled cDNA probe transcribed from a mixture of 16S and 23S rRNA from P. stuartii NCTC 11800T. The pattern of bands (the rDNA fingerprint), which depended on restriction fragment length polymorphism containing rRNA genes, was used as a measure of minor genomic variation within and between species. The P. stuartii clinical isolates had similar total digest patterns, but the rDNA fingerprints revealed some heterogeneity between strains, with EcoRI digests providing better strain discrimination than HindIII. Such rDNA fingerprints comprised between five and seven bands with sizes in the range of 5 to 28 kilobases. The 11 different EcoRI patterns were compared by numerical analysis, and several groups or subgroups of strains were identified. Over half (15 of 26) of the urease-negative isolates (subgroups Aa and Ab) had patterns that differed only by the presence or absence of a 25-kilobase band. Urease-negative strains from other clinical material were more heterogeneous in their patterns. No correlation was apparent between strain pattern group and urease production or geographic location of isolate. The P. stuartii rDNA fingerprints were quite distinct from those of allied Providencia and Proteus species and provided a more sensitive measure of minor genomic differences than total DNA digests did.

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“…Bacterialstrains andgrowth conditions. Experiments were done with P. stuartii strains P308 and P306 (Hawkey et al, 1985;Chopra et al, 1987). The latter carries the plasmid pUB2660, which encodes a TEM2-type P-lactamase (Hawkey et al, 1985).…”

Section: Methodsmentioning

confidence: 99%

“…Experiments were done with P. stuartii strains P308 and P306 (Hawkey et al, 1985;Chopra et al, 1987). The latter carries the plasmid pUB2660, which encodes a TEM2-type P-lactamase (Hawkey et al, 1985). Bacteria were grown aerobically at 37 "C either in a nutrient broth (Lab M broth; London Analytical and Bacteriological Media) or on nutrient agar (Lab M agar; London Analytical and Bacteriological Media).…”

Section: Methodsmentioning

confidence: 99%

Fractionation of the Providencia stuartii Cell Envelope

Chopra

Johnson²

1987

Microbiology

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Cell envelopes (i.e. unfractionated inner and outer membranes) were obtained from Providencia stuartii by following procedures previously applied to the isolation of envelopes from Escherichia coli. The P. stuartii envelopes contained known inner membrane enzymes that included a variety of dehydrogenases and ATPase. The catalytic activity of the ATPase depended upon the concentration of magnesium ions, the substrate (ATP) level and the ratio of magnesium ions to ATP. Cell envelopes from P. stuartii were further fractionated to recover inner and outer membrane polypeptides by treatment with the detergent Sarkosyl. Proteins from the periplasmic region were recovered by a simple osmotic shock procedure also previously applied to E. coli. The purity of the various P. stuartii cell envelope fractions was assessed by a combination of techniques that included one- and two-dimensional gel electrophoresis of proteins, enzyme assays and detection of penicillin-binding proteins.

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Evolution of an R Plasmid from a Cryptic Plasmid by Transposition of Two Copies of Tn1 in Providencia stuartii

Cited by 9 publications

References 19 publications

Structures of Homologous Composite Transposons Carrying cbaABC Genes from Europe and North America

Structures of Homologous Composite Transposons Carrying cbaABC Genes from Europe and North America

Detection of genomic variation in Providencia stuartii clinical isolates by analysis of DNA restriction fragment length polymorphisms containing rRNA cistrons

Fractionation of the Providencia stuartii Cell Envelope

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