Poly(A)-binding protein (PABP) is a major component of the messenger RNA–protein complex. PABP is able to bind the poly(A) tail of mRNA, as well as translation initiation factor 4G and eukaryotic release factor 3a (eRF3a). PABP has been found to stimulate translation initiation and to inhibit nonsense-mediated mRNA decay. Using a reconstituted mammalian in vitro translation system, we show that PABP directly stimulates translation termination. PABP increases the efficiency of translation termination by recruitment of eRF3a and eRF1 to the ribosome. PABP's function in translation termination depends on its C-terminal domain and its interaction with the N-terminus of eRF3a. Interestingly, we discover that full-length eRF3a exerts a different mode of function compared to its truncated form eRF3c, which lacks the N-terminal domain. Pre-association of eRF3a, but not of eRF3c, with pre-termination complexes (preTCs) significantly increases the efficiency of peptidyl–tRNA hydrolysis by eRF1. This implicates new, additional interactions of full-length eRF3a with the ribosomal preTC. Based on our findings, we suggest that PABP enhances the productive binding of the eRF1–eRF3 complex to the ribosome, via interactions with the N-terminal domain of eRF3a which itself has an active role in translation termination.
Past seismic catastrophes were unknown in Sakhalin Island before 1995 except those suggested from findings of paleoseismodislocations. The first time that dwellers have experienced such a catastrophe in the Sakhalin Island history was on 27 May 1995. The devastating Neftegorsk earthquake occurred in Northern Sakhalin (cp = 52.8" north; h = 143.2' east; H = 18 km; M, = 7.2), killed almost 2000 people in the small city of Neftegorsk, caused damage and destruction of buildings, bridges, railways and roads, breakage of oil and gas pipelines, electric and communication lines, and was accompanied by large-scale surface phenomena within a source area. It was felt all over the Sakhalin Island, as well as over the closest part of the Eurasian continent. Surface fracturing was the most impressive effect of the Neftegorsk earthquake. The 37-km long, right-lateral strike-slip fault, with a strike of north 15" east and a horizontal displacement up to a maximum of 8m, has been observed from Taxon Mountain at the south to the junction of the Cadylanye and Keniga Rivers at the north. According to the results of a detailed geological survey and study of the aftershocks, the total extent of the source area was -80 km. Various secondary phenomena have been observed at the Earth's surface, such as landslides, falls, soil liquefaction, mud volcanoes etc. The earthquake was followed by hundreds of aftershocks within the following 1-2 months. Spatially, the earthquake fault coincides with the pre-existing Upper Piltun fault, known earlier from geological studies. Recent high activity of the latter fault has been recognized only after the Neftegorsk event because of findings of traces of significant past dislocations within the fault zone. From a tectonic viewpoint it can be suggested that the Upper Piltun fault is a Riedel-type shear fracture located between two main regional faults: the Gyrgylanye-Dagy fault at the west and the Piltun-Ekhaby fault at the east. Therefore, its present activity, expressed by the destructive Neftegorsk earthquake, seems to be explained by a long strain accumulation within a broad zone of regional right-lateral shear faulting.
scite is a Brooklyn-based organization that helps researchers better discover and understand research articles through Smart Citations–citations that display the context of the citation and describe whether the article provides supporting or contrasting evidence. scite is used by students and researchers from around the world and is funded in part by the National Science Foundation and the National Institute on Drug Abuse of the National Institutes of Health.